TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc.

Zeynep DANIŞMAN; Şule İNAN; Esma ÖZSOY; Emre YÖRÜK; Tapani YLİ-MATTİLA

doi:https://doi.org/10.23902/trkjnat.1000196

Article Details

TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc.

Authors:

Zeynep DANIŞMAN | Şule İNAN | Esma ÖZSOY | Emre YÖRÜK | Tapani YLİ-MATTİLA

Publisher:

Trakya University

Main Topics:

Animal husbandry | Life sciences | Genetics and evolution

Journal:

Trakya University Journal of Natural Sciences 2022 Vol.23 Issue 1, pp.71-79

Document Type:

Article

Publication Date:

4/2022

Document Language:

English

Document ID (Org No.):

2000678986

Frequency:

Bi-Annual

eISSN:

2528-9691

DOI:

https://doi.org/10.23902/trkjnat.1000196

Abstract:

 

Fusarium avenaceum (Fr.) Sacc. is an important phytopathogen. Fight against F. avenaceum includes primarily fungicide usage. However, novel strategies are needed in a struggle with F. avenaceum. In this study, terpinolene was used against F. avenaceum as an antimicrobial agent, and the harmlessness of terpinolene was tested on two contrast barley genotypes, Hordeum vulgare L. cv. Cervoise and H. vulgare L. cv. Premium. Firstly, minimum inhibitory concentration (MIC) and half inhibitory concentration (IC50) were detected as 6 and 3 µg µL-1. Quantitative real-time polymerase chain reaction (qRT-PCR) assay was informative about the effects of terpinolene on enniatin and zearalenone biosynthesis in F. avenaceum. Terpinolene leads to the potential decreased enniatin and zearalenone biosynthesis in F. avenaceum. However, no significant differences were recorded for gene expression of aurofusariun biosynthesis. In barley, electroconductivity (EC), catalase (CAT) activity, coupled restriction enzyme digestion-random amplification (CRED-RA), and qRT-PCR assays were tested. No significant ion leakage differences (p>0.05) were detected. Similarly, CAT activity and water loss rate (WLR) values in barley sets were not affected (p>0.05) by terpinolene treatment in majority of experiment sets. Relatively low levels of genomic template instability (75-85%) and epigenetics changes (11-20.6%) were recorded in barley due to terpinolene treatment. WRKY6, WRKY24, and WRKY41 expressions were not significantly changed. The findings showed that terpinolene could be accepted as a potential plant protective agent against phytopathogenic fungi. .

 


Fusarium avenaceum (Fr.) Sacc. önemli bir bitki patojenidir. Fusarium avenaceum ile mücadele temelde fungisit kullanımını içerir. Ancak yeni stratejilere ihtiyaç duyulmaktadır. Mevcut çalışmada iki zıt arpa genotipi olan Hordeum vulgare L. cv. Cervoise and H. vulgare L. cv. Premium genotiplerinde terpinolenin zararsız etkileri ve F. avenaceum’a karşı antimikrobiyal etkileri araştırıldı. İlk olarak minimum inhibisyon konsantrasyonları (MİK) ve üremeyi baskılayan yarı konsantrasyon (IC50) değerleri 6 and 3 µg µL-1 olarak belirlendi. Gerçek zamanlı polimeraz zincir reaksiyonu (kPZR) F. avenaceum’da zearalenone ve enniatin üretimi üzerinde terpinolenin etkisinin belirlenmesinde bilgi verici oldu. Terpinolene F. avenaceum’da enniatin ve zearalenone üretiminde potansiyel baskılamaya sebep oldu. Buna karşın aurofusariun biyosentez gen analtımında anlamlı farklılık görülmemiştir. Arpada elektrokonduktivite (EK), katalaz (KAT) aktivitesi, CRED-RA analizi ve kPZR analizleri test edildi. EK değerlerinde bilimsel olarak anlamlı (p>0,05) farklılık gözlemlenmedi. Benzer şekilde deney setlerinin çoğunda katalaz aktivitesi ve su kaybetme oranları (SKO) terpinolene uygulamasından etkilenmedi (p>0,05). Arpada terpinolene uygulamasına bağlı olarak göreceli olarak düşük genomik instabilite (%75-85) ve epigenetik değişimler (%11-20,6) kaydedildi. WRKY6, WRKY24 ve WRKY41 gen anlatımları anlamlı farklılık göstermedi. Bulgular terpinolenin fitopatojenik mantarlara karşı potansiyel bir bitki koruma ajanı olarak kabul edilebileceğini göstermektedir. 

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Article Details

TERPINOLENE IS AN EFFECTIVE ESSENTIAL OIL COMPOUND TO PROTECT Hordeum vulgare L. FROM Fusarium avenaceum (Fr.) Sacc.

Authors: Zeynep DANIŞMAN | Şule İNAN | Esma ÖZSOY | Emre YÖRÜK | Tapani YLİ-MATTİLA

Publisher: Trakya University

Main Topics: Animal husbandry | Life sciences | Genetics and evolution

Journal: Trakya University Journal of Natural Sciences 2022 Vol.23 Issue 1, pp.71-79

Document Type: Article

Publication Date: 4/2022

Document Language: English

Document ID (Org No.): 2000678986

Frequency: Bi-Annual

eISSN: 2528-9691

DOI: https://doi.org/10.23902/trkjnat.1000196

Issues

Authors:

Zeynep DANIŞMAN | Şule İNAN | Esma ÖZSOY | Emre YÖRÜK | Tapani YLİ-MATTİLA

Publisher:

Trakya University

Main Topics:

Animal husbandry | Life sciences | Genetics and evolution

Journal:

Trakya University Journal of Natural Sciences 2022 Vol.23 Issue 1, pp.71-79

Document Type:

Article

Publication Date:

4/2022

Document Language:

English

Document ID (Org No.):

2000678986

Frequency:

Bi-Annual

eISSN:

2528-9691

DOI:

https://doi.org/10.23902/trkjnat.1000196