Genospecies and virulence factors of Aeromonas species in different sources in a North African country

KS Ghenghesh; SF Ahmed; P Cappuccinelli; JD Klena

doi:10.3402/ljm.v9.25497

Article Details

Genospecies and virulence factors of Aeromonas species in different sources in a North African country

Authors:

KS Ghenghesh | SF Ahmed | P Cappuccinelli | JD Klena

Publisher:

Libyan Journal of Medicine

Journal:

Libyan Journal of Medicine Vol.9 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

7/2014

Document Language:

English

Document ID (Org No.):

2001392668

pISSN:

1819-6357

DOI:

10.3402/ljm.v9.25497

Keywords:

Abstract:

 Introduction: Aeromonads of medical importance have been reported from numerous clinical, food, and water sources, but identification of genospecies and virulence factors of Aeromonas species from countries in North Africa and the Middle East are few.Methods: In total 99 Aeromonas species isolates from different sources (diarrheal children [n=23], nondiarrheal children [n=16], untreated drinking water from wells [n=32], and chicken carcasses [n=28]) in Tripoli, Libya, were included in the present investigation. Genus identification was confirmed by biochemical analysis, and genospecies were determined using a combination of 16S rDNA variable region and gyrB sequence analysis. Polymerase chain reaction (PCR) was used to detect genes encoding toxins from 52 of the isolates.Results: We identified 44 isolates (44%) as A. hydrophila (3 [3.0%] subspecies anaerogenes, 23 [23%] subspecies dhakensis, and 18 [18%] subspecies ranae); 27 isolates (27%) as A. veronii; 23 isolates (23%) as A. caviae; and 5 isolates (5.0%) as other genospecies. The genes encoding aerolysin (aer), cytolytic enterotoxin (act), and A. hydrophila isolate SSU enterotoxin (ast) were detected in 45 (87%), 4 (7.7%), and 9 (17%) of the 52 isolates tested, respectively. The gene encoding an extracellular lipase (alt) was not detected.Conclusion: The majority of aeromonads from Libya fall within three genospecies (i.e. A. hydrophila, A. veronii, and A. caviae), and genes coding for toxin production are common among them.Keywords: Aeromonas; genospecies; virulence factors; Libya.

Article Details

Genospecies and virulence factors of Aeromonas species in different sources in a North African country

Authors: KS Ghenghesh | SF Ahmed | P Cappuccinelli | JD Klena

Publisher: Libyan Journal of Medicine

Journal: Libyan Journal of Medicine Vol.9 Issue 1, pp.0-0

Document Type: Article

Publication Date: 7/2014

Document Language: English

Document ID (Org No.): 2001392668

pISSN: 1819-6357

DOI: 10.3402/ljm.v9.25497

Aeromonas

genospecies

virulence factors

Libya

Issues

Authors:

KS Ghenghesh | SF Ahmed | P Cappuccinelli | JD Klena

Publisher:

Libyan Journal of Medicine

Journal:

Libyan Journal of Medicine Vol.9 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

7/2014

Document Language:

English

Document ID (Org No.):

2001392668

pISSN:

1819-6357

DOI:

10.3402/ljm.v9.25497