Effect of L-ascorbate/Cu(II) on growth of Bacillus subtilis and Escherichia coli

S. TAN

doi:https://doi.org/10.1501/Commuc_0000000091

Article Details

Effect of L-ascorbate/Cu(II) on growth of Bacillus subtilis and Escherichia coli

Authors:

S. TAN

Publisher:

Ankara University

Main Topics:

Life sciences

Journal:

Communications Faculty of Sciences University of Ankara Series C Biology 1999 Vol.17 (1999), pp.17-0

Document Type:

Article

Publication Date:

12/1998

Document Language:

English

Document ID (Org No.):

2000911370

Frequency:

Monthly

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/Commuc_0000000091

Abstract:

 

Antiviral and antitumoral effect of L-ascorbate (L-AsA) / Cu (II) have been established. We studied the antibacterial effect of L-ascorbate / Cu (II) and its action on the bacterial celi membrane.
Oxidation products of ascorbate (AsA) produced in the presence of Cu (II). It has been known that these oxidation products cause breaks in protein and nucleic acids. They also lead lipid peroxidation. Effects of different concentrations of AsA + CuCl2 on grovvth of Escherichia cali and Bacillus subtilis were investigated. Also subtoxic concentration of AsA / Cu (II) that affect bacterial growth were established.
It was found that the inhibitory concentration for B. subtilis was 4 mM AsA +100 |Ll M CuCl2 and E. coli was 8mM AsA+lOOJlM CuClD. The growth of bacterial cells were follovved spectrophotometrically.
Also viable celi counts were made.
Bacterial membranes isolated by Lysozyme-EDTA method to show changes caused in membranes.
Also membranes isolated from control cells and bacteria grown in AsA+CuCf medium were applied on SDS-PAGE to show the changes occured in the membranes.

Article Details

Effect of L-ascorbate/Cu(II) on growth of Bacillus subtilis and Escherichia coli

Authors: S. TAN

Publisher: Ankara University

Main Topics: Life sciences

Journal: Communications Faculty of Sciences University of Ankara Series C Biology 1999 Vol.17 (1999), pp.17-0

Document Type: Article

Publication Date: 12/1998

Document Language: English

Document ID (Org No.): 2000911370

Frequency: Monthly

eISSN: 2651-3749

pISSN: 1303-6025

DOI: https://doi.org/10.1501/Commuc_0000000091

Issues

Authors:

S. TAN

Publisher:

Ankara University

Main Topics:

Life sciences

Journal:

Communications Faculty of Sciences University of Ankara Series C Biology 1999 Vol.17 (1999), pp.17-0

Document Type:

Article

Publication Date:

12/1998

Document Language:

English

Document ID (Org No.):

2000911370

Frequency:

Monthly

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/Commuc_0000000091