The Effect of Intracerebroventricular Nesfatin-1 Injection on Serum Ghrelin, Leptin, Orexin A, and Asprosin Levels in Male Rats

Fatmanur Selim; Ömer Faruk Kalkan; Ali Yavuz Uzun; Ahmet Alver; Zafer Şahin

Article Details

The Effect of Intracerebroventricular Nesfatin-1 Injection on Serum Ghrelin, Leptin, Orexin A, and Asprosin Levels in Male Rats

Authors:

Fatmanur Selim | Ömer Faruk Kalkan | Ali Yavuz Uzun | Ahmet Alver | Zafer Şahin

Publisher:

Gumushane University

Main Topics:

Endokrinoloji

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.878-885

Document Type:

Article

Publication Date:

9/2025

Document Language:

Turkish

Document ID (Org No.):

2001358870

pISSN:

2146-9954

Keywords:

Abstract:

 Nesfatin-1 is an anorexigenic peptide produced in hypothalamic regions. The anorexigenic effect of nesfatin-1 involves not only the activation of the hypothalamic-pituitary-adrenal HPA axis but also the stress response. Although nesfatin-1 is known to influence appetite-regulating neuropeptides, its specific effects on leptin, ghrelin, orexin-A, and asprosin have yet to be fully characterized. This study was conducted to explore how nesfatin-1 interacts with CRH1, CRH2, and oxytocin receptor antagonists to affect the serum concentrations of these neuropeptides.In this study, a total of 35 animals were used and randomly divided into 5 groups n7 per group: sham control, nesfatin-1, nesfatin-1 25 pmol  P234 1 nmol, nesfatin-1  antalarmin 0.1 μg, nesfatin-1  astressin 2B 1 μg, and nesfatin-1  atosiban 300 ng. All antagonists were administered via the intracerebroventricular route. The animals were sacrificed by decapitation 30 minutes after anesthesia 1.25 gkg, intraperitoneal. Serum samples were collected, and levels of leptin, ghrelin, orexin A, and asprosin were measured using commercial ELISA kits. Administration of nesfatin-1 alone did not significantly alter serum leptin, ghrelin, orexin-A, or asprosin levels p0.05. Co-administration of nesfatin-1 with P234 reduced orexin-A and ghrelin levels while increasing leptin levels p0.05. Co-administration of nesfatin-1 with antalarmin, astressin 2B, or atosiban resulted in a significant decrease in serum orexin-A levels p0.05. These findings suggest that the effects of nesfatin-1 on neuropeptides regulating hunger and satiety are modulated through receptor-level interactions. In particular, the observed decrease in orexin-A levels following antagonism of CRH1, CRH2, and oxytocin receptors indicates that nesfatin-1 may exert indirect inhibitory effects on orexigenic systems.

 Nesfatin-1 is an anorexigenic peptide produced in hypothalamic regions. The anorexigenic effect of nesfatin-1 involves not only the activation of the hypothalamic-pituitary-adrenal HPA axis but also the stress response. Although nesfatin-1 is known to influence appetite-regulating neuropeptides, its specific effects on leptin, ghrelin, orexin-A, and asprosin have yet to be fully characterized. This study was conducted to explore how nesfatin-1 interacts with CRH1, CRH2, and oxytocin receptor antagonists to affect the serum concentrations of these neuropeptides.In this study, a total of 35 animals were used and randomly divided into 5 groups n7 per group: sham control, nesfatin-1, nesfatin-1 25 pmol  P234 1 nmol, nesfatin-1  antalarmin 0.1 μg, nesfatin-1  astressin 2B 1 μg, and nesfatin-1  atosiban 300 ng. All antagonists were administered via the intracerebroventricular route. The animals were sacrificed by decapitation 30 minutes after anesthesia 1.25 gkg, intraperitoneal. Serum samples were collected, and levels of leptin, ghrelin, orexin A, and asprosin were measured using commercial ELISA kits. Administration of nesfatin-1 alone did not significantly alter serum leptin, ghrelin, orexin-A, or asprosin levels p0.05. Co-administration of nesfatin-1 with P234 reduced orexin-A and ghrelin levels while increasing leptin levels p0.05. Co-administration of nesfatin-1 with antalarmin, astressin 2B, or atosiban resulted in a significant decrease in serum orexin-A levels p0.05. These findings suggest that the effects of nesfatin-1 on neuropeptides regulating hunger and satiety are modulated through receptor-level interactions. In particular, the observed decrease in orexin-A levels following antagonism of CRH1, CRH2, and oxytocin receptors indicates that nesfatin-1 may exert indirect inhibitory effects on orexigenic systems.

 Nesfatin-1 is an anorexigenic peptide produced in hypothalamic regions. The anorexigenic effect of nesfatin-1 involves not only the activation of the hypothalamic-pituitary-adrenal HPA axis but also the stress response. Although nesfatin-1 is known to influence appetite-regulating neuropeptides, its specific effects on leptin, ghrelin, orexin-A, and asprosin have yet to be fully characterized. This study was conducted to explore how nesfatin-1 interacts with CRH1, CRH2, and oxytocin receptor antagonists to affect the serum concentrations of these neuropeptides.In this study, a total of 35 animals were used and randomly divided into 5 groups n7 per group: sham control, nesfatin-1, nesfatin-1 25 pmol  P234 1 nmol, nesfatin-1  antalarmin 0.1 μg, nesfatin-1  astressin 2B 1 μg, and nesfatin-1  atosiban 300 ng. All antagonists were administered via the intracerebroventricular route. The animals were sacrificed by decapitation 30 minutes after anesthesia 1.25 gkg, intraperitoneal. Serum samples were collected, and levels of leptin, ghrelin, orexin A, and asprosin were measured using commercial ELISA kits. Administration of nesfatin-1 alone did not significantly alter serum leptin, ghrelin, orexin-A, or asprosin levels p0.05. Co-administration of nesfatin-1 with P234 reduced orexin-A and ghrelin levels while increasing leptin levels p0.05. Co-administration of nesfatin-1 with antalarmin, astressin 2B, or atosiban resulted in a significant decrease in serum orexin-A levels p0.05. These findings suggest that the effects of nesfatin-1 on neuropeptides regulating hunger and satiety are modulated through receptor-level interactions. In particular, the observed decrease in orexin-A levels following antagonism of CRH1, CRH2, and oxytocin receptors indicates that nesfatin-1 may exert indirect inhibitory effects on orexigenic systems.

 Hipotalamik alanlarda üretilen nesfatin-1 anoreksijenik bir peptittir. Nesfatin-1in bu anoreksijenik etkisinde hipotalamo-hipofizer-adrenal aks aktivasyonu yanı sıra stres yanıtı da rol oynar. Nesfatin-1in leptin, ghrelin, orexin-A ve asprosin gibi açlık-tokluk üzerinde önemli rol oyanayan nöropeptitler üzerinde nasıl bir etkiye sahip olduğu tam olarak aydınlatılmamıştır. Bu çalışmada, nesfatin-1in CRH1, CRH2 ve oksitosin reseptör antagonistleriyle etkileşiminin, serum ghrelin, leptin, asprosin ve oreksin A düzeyleri üzerindeki etkilerini araştırmak hedeflenmiştir. Çalışmada toplam 42 adet erkek sıçan kullanılmış ve rastgele 6 gruba ayrılmıştır; her grup için n7: sham kontrol, nesfatin-1, nesfatin-1 25pmol P234 1 nmol, nesfatin-1antalarmin 0,1 μg, nesfatin-1 astressin 1 μg 2B, nesfatin-1atosiban 300 ng. Tüm antagonistler introserebroventriküler yolla enjekte edilmiştir. Hayvanlar anestezi 1.25 gkg, periton içi altına alındıktan 30 dakika sonra dekapitasyonla sakrifiye edilmiştir. Serum örnekleri toplanmış ve leptin, ghrelin, orexin A ve asprosin seviyeleri ELISA ticari kitleri ile ölçülmüştür. Nesfatin-1 tek başına uygulaması serum leptin, ghrelin, orexin-A ve asprosin seviyelerini değiştirmemiştir p0.05. Nesfatin-1in P234 ile uygulaması orexin-A ve ghrelin seviyelerini azaltırken leptin seviyesini artırmıştır p0.05. Nesfatin-1in antalarmin, astressin 2B ve atosiban ile uygulanması serum orexin-a seviyesini azaltmıştır p0.05. Elde edilen bulgular, nesfatin-1in açlık-tokluk düzenleyici nöropeptidler üzerindeki etkilerinin, reseptör düzeyindeki etkileşimler aracılığıyla modüle edildiğini göstermektedir. Özellikle CRH1, CRH2 ve oksitosin reseptörlerinin antagonizmi sonrasında gözlenen orexin-A düzeylerindeki düşüş, nesfatin-1in oreksijenik sistemler üzerinde dolaylı inhibitör etkiler gösterebileceğini göstermektedir.

Article Details

The Effect of Intracerebroventricular Nesfatin-1 Injection on Serum Ghrelin, Leptin, Orexin A, and Asprosin Levels in Male Rats

Authors:

Fatmanur Selim | Ömer Faruk Kalkan | Ali Yavuz Uzun | Ahmet Alver | Zafer Şahin

Publisher:

Gumushane University

Main Topics:

Endokrinoloji

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.878-885

Document Type:

Article

Publication Date:

9/2025

Document Language:

Turkish

Document ID (Org No.):

2001358870

pISSN:

2146-9954

Nesfatin-1

Ghrelin

Leptin

Orexin A

AsprosinNesfatin-1

Ghrelin

Leptin

Oreksin A

Asprosin

Issues

Article Details

Authors: Fatmanur Selim | Ömer Faruk Kalkan | Ali Yavuz Uzun | Ahmet Alver | Zafer Şahin

Publisher: Gumushane University

Main Topics: Endokrinoloji

Journal: Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.878-885

Document Type: Article

Publication Date: 9/2025

Document Language: Turkish

Document ID (Org No.): 2001358870

pISSN: 2146-9954

Issues

Authors:

Fatmanur Selim | Ömer Faruk Kalkan | Ali Yavuz Uzun | Ahmet Alver | Zafer Şahin

Publisher:

Gumushane University

Main Topics:

Endokrinoloji

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.878-885

Document Type:

Article

Publication Date:

9/2025

Document Language:

Turkish

Document ID (Org No.):

2001358870

pISSN:

2146-9954