Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Demet Çelebi; Özgür Çelebi; Sümeyye Başer; Ali Taghizadehghalehjoughi

Article Details

Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Authors:

Demet Çelebi | Özgür Çelebi | Sümeyye Başer | Ali Taghizadehghalehjoughi

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2277-2284

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346295

pISSN:

2630-6344

Keywords:

Abstract:

 Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration MIC values were determined on Streptococcus mitis at doses of 31.25 µgml of Sodium Perborate Metahydrate SPM, Zinc Borate ZB 62.5 µgml and Etidote 125 µgml. EtidoteSPM, EtidoteZB, and ZBSPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µgml32 µgml in all combinations, while it showed an effect on biofilm at doses of 1028 µgml 1028 µgml HepG2 Cell culture.

 Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration MIC values were determined on Streptococcus mitis at doses of 31.25 µgml of Sodium Perborate Metahydrate SPM, Zinc Borate ZB 62.5 µgml and Etidote 125 µgml. EtidoteSPM, EtidoteZB, and ZBSPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µgml32 µgml in all combinations, while it showed an effect on biofilm at doses of 1028 µgml 1028 µgml HepG2 Cell culture.

 Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration MIC values were determined on Streptococcus mitis at doses of 31.25 µgml of Sodium Perborate Metahydrate SPM, Zinc Borate ZB 62.5 µgml and Etidote 125 µgml. EtidoteSPM, EtidoteZB, and ZBSPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µgml32 µgml in all combinations, while it showed an effect on biofilm at doses of 1028 µgml 1028 µgml HepG2 Cell culture.

 Liver abscesses are most commonly attributed to Escherichia coli and Klebsiella pneumoniae, but because Streptococcus mitis is a commensal bacterium with low virulence activity, it is overlooked by its antimicrobial resistance and virulence factors. In this study, we tried to evaluate the synergistic effect of boron compounds and the effect on biofilm in the infection model we created with Streptococcus mitis NCTC 12261 on the HepG2 liver cell line. Minimal inhibitory concentration MIC values were determined on Streptococcus mitis at doses of 31.25 µgml of Sodium Perborate Metahydrate SPM, Zinc Borate ZB 62.5 µgml and Etidote 125 µgml. EtidoteSPM, EtidoteZB, and ZBSPM combinations showed the most effective fractional inhibitor concentration value synergistic effect at minimum doses of 32 µgml32 µgml in all combinations, while it showed an effect on biofilm at doses of 1028 µgml 1028 µgml HepG2 Cell culture.

Article Details

Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Authors:

Demet Çelebi | Özgür Çelebi | Sümeyye Başer | Ali Taghizadehghalehjoughi

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2277-2284

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346295

pISSN:

2630-6344

Biofilm

Boron Compounds

Liver Cell

Synergistic Effect

Cytotoxic effect

Issues

Article Details

Investigation of the antibacterial, antibiofilm and cytotoxic effects of boron compounds in a Streptococcus mitis infection model on HepG2 liver cell

Authors: Demet Çelebi | Özgür Çelebi | Sümeyye Başer | Ali Taghizadehghalehjoughi

Publisher: Marmara University

Main Topics: Pharmacology and Pharmaceutical Sciences (Other)

Journal: Journal of Research in Pharmacy Vol.27 Issue 6, pp.2277-2284

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001346295

pISSN: 2630-6344

Biofilm

Boron Compounds

Liver Cell

Synergistic Effect

Cytotoxic effect

Issues

Authors:

Demet Çelebi | Özgür Çelebi | Sümeyye Başer | Ali Taghizadehghalehjoughi

Publisher:

Marmara University

Main Topics:

Pharmacology and Pharmaceutical Sciences (Other)

Journal:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2277-2284

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001346295

pISSN:

2630-6344