Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Ayesha Ahmed; Dalal M. Al-Tamimi

doi:10.1080/19932820.2018.1466573

Article Details

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Authors:

Ayesha Ahmed | Dalal M. Al-Tamimi

Publisher:

Libyan Journal of Medicine

Journal:

Libyan Journal of Medicine Vol.13 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

5/2018

Document Language:

English

Document ID (Org No.):

2001392736

pISSN:

1819-6357

DOI:

10.1080/19932820.2018.1466573

Keywords:

Abstract:

 Her2-neu overexpression has a pathogenetic, therapeutic and a controversial prognostic role in gastric cancer. p-53 mutation status and Ki-67 proliferation index are established prognostic markers in many tumors. In this study we evaluated p-53 and Ki-67 in relation to Her2-neu positive and negative gastric adenocarcinoma (GA). This cross-sectional study was carried out at King Fahd Hospital of Imam Abdulrahman bin Faisal University. Fifty cases ofGAwere retrieved from pathology archives. Clinico-pathological parameters were evaluated. Immunohistochemical protein analysis for Her2-neu, Ki-67 and p-53 was carried out. Fluorescent in situ hybridization (FISH) analysis was done for Her2-neu positive cases showing 2+ immunoexpression. Frequency of Ki-67 and p-53 positivity in Her2-neu positive cases was calculated and compared with those in Her2-neu negative cases. Correlation of clinicopatological parameters with Her2 positive and negative cases, p-53 mutation status and Ki-67 proliferation index was carried out. Her2-neu overexpression was present in 12% (n = 6) cases. A high Ki-67 was seen predominantly in Her2-neu positive cases (83%, n = 5). Her2-neu negative cases (n = 44) showed moderate (31.88%, n = 14) to low (34%, n = 15) Ki-67. Diffuse p-53 positivity was seen predominantly in Her2-neu positive cases (33.33%, n=2). Focalp-53 was seen mainly in Her2-neu negative cases 56.8% (n = 25). Negative p-53 was seen to be independent of Her2-neu status. Her2-neu positivity is strongly associated with diffuse p-53 mutation status and high Ki-67 proliferation. Her 2-neu negative status is associated with focal p-53 positivity and low to moderate Ki-67 proliferation index. Such stratifications in prognostic markers could not only be predictive in patient’s prognostics but could also form a basis of molecular classification of gastric cancer.Keywords: p-53; Ki-67; Her2-neu; gastric adenocarcinoma.

Article Details

Incorporation of p-53 mutation status and Ki-67 proliferating index in classifying Her2-neu positive gastric adenocarcinoma

Authors: Ayesha Ahmed | Dalal M. Al-Tamimi

Publisher: Libyan Journal of Medicine

Journal: Libyan Journal of Medicine Vol.13 Issue 1, pp.0-0

Document Type: Article

Publication Date: 5/2018

Document Language: English

Document ID (Org No.): 2001392736

pISSN: 1819-6357

DOI: 10.1080/19932820.2018.1466573

p-53

Ki-67

Her2-neu

gastric adenocarcinoma

Issues

Authors:

Ayesha Ahmed | Dalal M. Al-Tamimi

Publisher:

Libyan Journal of Medicine

Journal:

Libyan Journal of Medicine Vol.13 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

5/2018

Document Language:

English

Document ID (Org No.):

2001392736

pISSN:

1819-6357

DOI:

10.1080/19932820.2018.1466573