The Effect of Tarragon Extract Obtained by Different Extraction Methods on In Vitro Gas and Methane Production and Estimated Parameters in Dairy Cattle TMRs

Hasan Özsaygın; Adem Kaya

Article Details

The Effect of Tarragon Extract Obtained by Different Extraction Methods on In Vitro Gas and Methane Production and Estimated Parameters in Dairy Cattle TMRs

Authors:

Hasan Özsaygın | Adem Kaya

Publisher:

Erciyes University

Main Topics:

Hayvansal Üretim (Diğer)

Journal:

Erciyes Tarım ve Hayvan Bilimleri Dergisi Vol.8 Issue 1, pp.61-67

Document Type:

Article

Publication Date:

6/2025

Document Language:

Turkish

Document ID (Org No.):

2001338844

pISSN:

2651-5334

Keywords:

Abstract:

 This study was conducted to reveal the antimethanogenic effects of tarragon Artemisia dracunculus extracts obtained by different extraction methods using the in vitro gas production technique. Extracts obtained from tarragon using four different extraction methods water, acetone, ethanol, and methanol were added to the total mixed ration TMR of dairy cattle at a 1 level to form experimental groups. The rumen fluid used in the in vitro gas production technique was obtained postslaughter from a special slaughterhouse, sourced from Simmental cattle aged between 4-6 years and weighing approximately 500 kg. Compared to the control group, the groups supplemented with acetone, ethanol, and methanol extracts exhibited higher in vitro gas production values, as well as higher estimated parameters such as ME, NEL, and OMS, while no significant effect on methane production was detected P0.05. The NDF digestibility value determined using the Daisy incubator was highest in the ethanol group 36.01 and lowest in the acetone group 18.33 P0.05. Among the digestibility parameters, the partitioning factor PF and microbial protein synthesis efficiency MPSE values were found to be the lowest in the acetone group, at 2.43 and 9.33, respectively P0.05. In conclusion, it was determined that tarragon extracts affect rumen fermentation and energy efficiency, do not have a significant effect on methane production, but have the potential to improve feed value. It was concluded that higher doses should be tested to reveal the antimethanogenic effect of these extracts.

 This study was conducted to reveal the antimethanogenic effects of tarragon Artemisia dracunculus extracts obtained by different extraction methods using the in vitro gas production technique. Extracts obtained from tarragon using four different extraction methods water, acetone, ethanol, and methanol were added to the total mixed ration TMR of dairy cattle at a 1 level to form experimental groups. The rumen fluid used in the in vitro gas production technique was obtained postslaughter from a special slaughterhouse, sourced from Simmental cattle aged between 4-6 years and weighing approximately 500 kg. Compared to the control group, the groups supplemented with acetone, ethanol, and methanol extracts exhibited higher in vitro gas production values, as well as higher estimated parameters such as ME, NEL, and OMS, while no significant effect on methane production was detected P0.05. The NDF digestibility value determined using the Daisy incubator was highest in the ethanol group 36.01 and lowest in the acetone group 18.33 P0.05. Among the digestibility parameters, the partitioning factor PF and microbial protein synthesis efficiency MPSE values were found to be the lowest in the acetone group, at 2.43 and 9.33, respectively P0.05. In conclusion, it was determined that tarragon extracts affect rumen fermentation and energy efficiency, do not have a significant effect on methane production, but have the potential to improve feed value. It was concluded that higher doses should be tested to reveal the antimethanogenic effect of these extracts.

 This study was conducted to reveal the antimethanogenic effects of tarragon Artemisia dracunculus extracts obtained by different extraction methods using the in vitro gas production technique. Extracts obtained from tarragon using four different extraction methods water, acetone, ethanol, and methanol were added to the total mixed ration TMR of dairy cattle at a 1 level to form experimental groups. The rumen fluid used in the in vitro gas production technique was obtained postslaughter from a special slaughterhouse, sourced from Simmental cattle aged between 4-6 years and weighing approximately 500 kg. Compared to the control group, the groups supplemented with acetone, ethanol, and methanol extracts exhibited higher in vitro gas production values, as well as higher estimated parameters such as ME, NEL, and OMS, while no significant effect on methane production was detected P0.05. The NDF digestibility value determined using the Daisy incubator was highest in the ethanol group 36.01 and lowest in the acetone group 18.33 P0.05. Among the digestibility parameters, the partitioning factor PF and microbial protein synthesis efficiency MPSE values were found to be the lowest in the acetone group, at 2.43 and 9.33, respectively P0.05. In conclusion, it was determined that tarragon extracts affect rumen fermentation and energy efficiency, do not have a significant effect on methane production, but have the potential to improve feed value. It was concluded that higher doses should be tested to reveal the antimethanogenic effect of these extracts.

 Bu çalışma, farklı ekstraksiyon yöntemleriyle elde edilen tarhun Artemisia dracunculus ekstraktlarının in vitro gaz üretim tekniğiyle antimetanojenik etkilerini ortaya koymak amacıyla yapılmıştır. Tarhun bitkisinden su, aseton, etanol ve metanol olmak üzere dört farklı ekstraksiyon yöntemiyle elde edilen özütler süt sığırı toplam rasyon karşımı TMRna 1 seviyesinde olacak şekilde ilave edilerek deneme grupları oluşturulmuş, in vitro gaz üretim tekniğinde kullanılan rumen sıvısı ise özel bir kesimhaneden, 4-6 yaş aralığında ve yaklaşık 500 kg ağırlığındaki Simental ırkı sığırdan kesim sonrası temin edilmiştir. Kontrol grubuna kıyasla, aseton, etanol ve metanol ekstraktı eklenen gruplarda in vitro gaz üretim değerleri ile ME, NEL ve OMS gibi tahminlenen parametreler daha yüksek saptanırken, metan üretimi üzerine anlamlı bir etkisinin olmadığı tespit edilmiştir P0.05. Daisy inkübatörle belirlenen NDF sindirilebilirlik değeri en yüksek etanol grubunda 36,01 en düşük ise aseton grubunda 18,33 belirlenmiştir P0.05. Sindirilebilirlik parametrelerinden taksimat faktörü PF ve mikrobiyal protein sentezlenme etkinlik MPSE değerleri 2,43 ve 9,33 ile en düşük aseton grubunda saptanmıştır P0.05. Sonuç olarak, tarhun ekstraktlarının rumen fermantasyonu ve enerji verimliliği üzerinde etkili olduğu, metan üretimi üzerine anlamlı bir etksinin olmadığı ancak yem değerini iyileştirme potansiyeline sahip olduğu belirlenmiştir. Söz konusu ekstraktların antimetanojenik etkisini ortaya koyabilme adına daha yüksek dozların denenmesi gerektiği ve kanaatine varılmıştır.

Article Details

The Effect of Tarragon Extract Obtained by Different Extraction Methods on In Vitro Gas and Methane Production and Estimated Parameters in Dairy Cattle TMRs

Authors:

Hasan Özsaygın | Adem Kaya

Publisher:

Erciyes University

Main Topics:

Hayvansal Üretim (Diğer)

Journal:

Erciyes Tarım ve Hayvan Bilimleri Dergisi Vol.8 Issue 1, pp.61-67

Document Type:

Article

Publication Date:

6/2025

Document Language:

Turkish

Document ID (Org No.):

2001338844

pISSN:

2651-5334

Ekstraction method

ekstract

in vitro gas

methane

tarragonEkstraksiyon yöntemi

ekstrakt

in vitro gaz

metan

tarhun

Issues

Article Details

Authors: Hasan Özsaygın | Adem Kaya

Publisher: Erciyes University

Main Topics: Hayvansal Üretim (Diğer)

Journal: Erciyes Tarım ve Hayvan Bilimleri Dergisi Vol.8 Issue 1, pp.61-67

Document Type: Article

Publication Date: 6/2025

Document Language: Turkish

Document ID (Org No.): 2001338844

pISSN: 2651-5334

Issues

Authors:

Hasan Özsaygın | Adem Kaya

Publisher:

Erciyes University

Main Topics:

Hayvansal Üretim (Diğer)

Journal:

Erciyes Tarım ve Hayvan Bilimleri Dergisi Vol.8 Issue 1, pp.61-67

Document Type:

Article

Publication Date:

6/2025

Document Language:

Turkish

Document ID (Org No.):

2001338844

pISSN:

2651-5334