Effect of Chlorpyrifos and Neem Seed Extract ( Azadirechtein ) on Hepatopancreatic Cellular Structures of the Freshwater Crayfish Procambarus Clarkii

Abdel Menam K.; Mahmoud  A. S.; Zeinab Z. K.; Radwa M. S.

doi:10.12816/0016974

Article Details

Effect of Chlorpyrifos and Neem Seed Extract ( Azadirechtein ) on Hepatopancreatic Cellular Structures of the Freshwater Crayfish Procambarus Clarkii

Authors:

Abdel Menam K. | Mahmoud A. S. | Zeinab Z. K. | Radwa M. S.

Publisher:

Egyptian Society for the Development of Fisheries and Human Health

Main Topics:

Zoological sciences/Animals

Journal:

Egyptian Journal of Aquatic Biology and Fisheries 2015 Vol.19 Issue 2, pp.125-136

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

73276

Frequency:

Quarterly

pISSN:

11106131

DOI:

10.12816/0016974

Descriptors:

Keywords:

Abstract:

 Histological and ultrastructural studies on the normal structures of hepatopancreas of P. clarkii showed three main cell types, secretory, absorptive and fibrillar cells. The ultrastructural alterations in the hepatopancreas of the red swamp crayfish P. clarkii exposed to sublethal concentrations of Chlorprifos and neem seed extract (Azadirechtein) pesticides were studied. LC25of Chlorpyrifos (0.0075 ppm) produced deformation of the apical surface of absorptive cell, dispersedmicrovilli, mitochondrial swelling with ballooned cristae, deformed cisternae of RER, lytic and vacuolated cytoplasm and pyknosis of nuclei. On the other hand, LC25of Azadirechtein (4.99 ppm) caused deformation of mitochondria with the sparce of their cisternae, fragmentation of microvilli, ruptured RER beside the presence of autophagic vacuole containing dark granules and finally bizzar nuclei having segregated nucleoli and irregular clumps of their chromatin. Chlorpyrifos was found to be more toxic than Azadirechtein on P. clarkii.

Article Details

Effect of Chlorpyrifos and Neem Seed Extract ( Azadirechtein ) on Hepatopancreatic Cellular Structures of the Freshwater Crayfish Procambarus Clarkii

Authors:

Abdel Menam K. | Mahmoud A. S. | Zeinab Z. K. | Radwa M. S.

Publisher:

Egyptian Society for the Development of Fisheries and Human Health

Main Topics:

Zoological sciences/Animals

Journal:

Egyptian Journal of Aquatic Biology and Fisheries 2015 Vol.19 Issue 2, pp.125-136

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

73276

Frequency:

Quarterly

pISSN:

11106131

DOI:

10.12816/0016974

Descriptors:

CRUSTACEAN CULTURE

HEPATOCYSTIS

CHLORPYRIFOS

SEED EXTRACTION

PANCREAS

CRAYFISH

EGYPT

Procambarus Clarkii

Chlorpyrifos

Azadirechtein

Hepatopancreas

Ultrastructure

Issues

Article Details

Authors: Abdel Menam K. | Mahmoud A. S. | Zeinab Z. K. | Radwa M. S.

Publisher: Egyptian Society for the Development of Fisheries and Human Health

Main Topics: Zoological sciences/Animals

Journal: Egyptian Journal of Aquatic Biology and Fisheries 2015 Vol.19 Issue 2, pp.125-136

Document Type: Article

Publication Date: 4/2015

Document Language: English

Document ID (Org No.): 73276

Frequency: Quarterly

pISSN: 11106131

DOI: 10.12816/0016974

Descriptors:

Issues

Authors:

Abdel Menam K. | Mahmoud A. S. | Zeinab Z. K. | Radwa M. S.

Publisher:

Egyptian Society for the Development of Fisheries and Human Health

Main Topics:

Zoological sciences/Animals

Journal:

Egyptian Journal of Aquatic Biology and Fisheries 2015 Vol.19 Issue 2, pp.125-136

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

73276

Frequency:

Quarterly

pISSN:

11106131

DOI:

10.12816/0016974