Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye

N.A.

Article Details

Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye

Authors:

N.A.

Publisher:

Kastamonu University

Journal:

Kastamonu Üniversitesi Orman Fakültesi Dergisi Vol.23 Issue 1, pp.47-51

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285764

pISSN:

1303-2399

Keywords:

Abstract:

 Aim of study: In this study, the effects of plant growth regulators PGRs on shoot multiplication of Crepis bithynica Boiss. Asteraceae, an endangered rare species in Türkiye were investigated. Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park. Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog MS basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures. Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively. Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.

 Aim of study: In this study, the effects of plant growth regulators PGRs on shoot multiplication of Crepis bithynica Boiss. Asteraceae, an endangered rare species in Türkiye were investigated. Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park. Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog MS basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures. Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively. Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.

 Aim of study: In this study, the effects of plant growth regulators PGRs on shoot multiplication of Crepis bithynica Boiss. Asteraceae, an endangered rare species in Türkiye were investigated. Area of study: The plant samples were collected from Kastamonu Ilgaz Mountain National Park. Material and method: The nodal segments were used as explant and they were cultured on Murashige and Skoog MS basal media supplemented with different plant growth regulators. Morphological changes of propagated plants were monitored during 21 days in vitro cultures. Main results: All plant growth regulators induced shoot growth and development without callus formation. Additionally, the highest shoot number and length of shoot per explant were achieved in MS media supplemented with 4.4 µM 6-BA plus 0.5 µM NAA, and 4.9 µM 2iP plus 0.5 µM IBA, respectively. Highlights: This study is the first study on the effects of PGRs in shoot multiplication of C. bithynica, and the results obtained can be used micropropagation for the species.

 Çalışmanın amacı: Bu çalışmada, bitki büyüme düzenleyicilerinin PGRs Türkiyede tehlike altında nadir bir tür olan Crepis bithynica Boiss. Asteraceaenın sürgün çoğaltımı üzerine etkileri araştırılmıştır. Çalışma alanı: Bitki örnekleri, Kastamonu Ilgaz Dağı Milli Parkından toplanmıştır. Materyal ve yöntem: Nodal segmentler eksplant olarak kullanıldı ve bunlar farklı bitki büyüme düzenleyicileri ile desteklenmiş Murashige and Skoog MS temel besiyerlerinde kültüre edildi. Çoğaltılan bitkilerdeki morfolojik değişmeler 21 gün boyunca in vitro kültürlerde izlendi. Temel sonuçlar: Tüm bitki büyüme düzenleyicileri kallus oluşturmaksızın sürgün büyüme ve gelişmesini teşvik etmişlerdir. Bununla birlikte, eksplant başına en yüksek sürgün sayısı ve sürgün uzunluğu, sırasıyla 4.4 µM 6-BA ile 0.5 µM NAA ve 4.9 µM 2iP ile 0.5 µM IBA içeren MS besiyerlerinde elde edilmiştir. Araştırma vurguları: Bu çalışma, C. bithynicanın sürgün çoğaltımında PGRlerin etkileri üzerine ilk çalışmadır ve elde edilen sonuçlar türün mikroçoğaltımı için kullanılabilir.

Article Details

Effects of Plant Growth Regulators on Shoot Multiplication of Crepis bithynica (Asteraceae): An Endangered Rare Species in Türkiye

Authors:

N.A.

Publisher:

Kastamonu University

Journal:

Kastamonu Üniversitesi Orman Fakültesi Dergisi Vol.23 Issue 1, pp.47-51

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285764

pISSN:

1303-2399

Crepis bithynica

Plant Growth Regulators

Shoot Multiplication

Ex Situ Conservation

Crepis bithynica

Bitki Büyüme Düzenleyicileri

Sürgün Çoğaltımı

Ex Situ Koruma

Issues

Article Details

Authors: N.A.

Publisher: Kastamonu University

Journal: Kastamonu Üniversitesi Orman Fakültesi Dergisi Vol.23 Issue 1, pp.47-51

Document Type: Article

Publication Date: 3/2023

Document Language: English

Document ID (Org No.): 2001285764

pISSN: 1303-2399

Issues

Authors:

N.A.

Publisher:

Kastamonu University

Journal:

Kastamonu Üniversitesi Orman Fakültesi Dergisi Vol.23 Issue 1, pp.47-51

Document Type:

Article

Publication Date:

3/2023

Document Language:

English

Document ID (Org No.):

2001285764

pISSN:

1303-2399