Electrochemical Comparison of the Interaction of 5-Nitrouracil with Single- or Double-Stranded DNA at mercury and glassy carbon electrodes

Mohamed Sayed IBRAHİM; Hossieny Sameh Mohamed IBRAHİM; Moustafa Mohamed KAMAL; Yassin Mohamed TEMERK

doi:https://doi.org/10.5505/apjes.2014.46855

Article Details

Electrochemical Comparison of the Interaction of 5-Nitrouracil with Single- or Double-Stranded DNA at mercury and glassy carbon electrodes

Authors:

Mohamed Sayed IBRAHİM | Hossieny Sameh Mohamed IBRAHİM | Moustafa Mohamed KAMAL | Yassin Mohamed TEMERK

Publisher:

Akademik Perspektif Derneği

Main Topics:

Applied physics

Journal:

Academic Platform - Journal of Engineering and Science 2014 Vol.2 Issue 1, pp.1-8

Document Type:

Article

Publication Date:

3/2014

Document Language:

English

Document ID (Org No.):

2000875247

Frequency:

Monthly

eISSN:

2822-2385

DOI:

https://doi.org/10.5505/apjes.2014.46855

Keywords:

Abstract:

 

The interaction of the 5-Nitrouracil (5NU), with ss-, and ds-DNA was investigated electrochemically in absence and presence of copper ions by using cyclic voltammetry (CV) and differential pulse stripping voltammetry (DPSV) at hanging mercury drop electrode (HMDE) and glassy carbon electrode (GCE) surfaces. It was found that, in absence of copper ions, the addition of ss- or ds-DNA to a buffered solution of 5NU results in a decrease on the 5NU redox peak current with a remarkable change in the peak potential (ca. 150 mV vs. Ag/AgCl) at both electrodes. This means that, an interaction of 5NU molecules with both ss- and ds-DNA was observed. The results also demonstrate that a distinguish between ss-, and ds-DNA can be achieved in presence of copper ion through their interaction with 5NU. The binding constants of 5NU with ds-DNA at HMDE and GCE were determined through the changes on the 5NU redox peak currents (at HMDE, 1.45x10^5 and at GCE, 2.65x10^5). The calibration plot for the DNA determination was obtained through the corresponding decreases on the DPSV peak current of 5NU to different additions of DNA (ss- or ds-DNA) concentration levels at the optimum conditions.

 


The interaction of the 5-Nitrouracil (5NU), with ss-, and ds-DNA was investigated electrochemically in
absence and presence of copper ions by using cyclic voltammetry (CV) and differential pulse stripping
voltammetry (DPSV) at hanging mercury drop electrode (HMDE) and glassy carbon electrode (GCE)
surfaces. It was found that, in absence of copper ions, the addition of ss- or ds-DNA to a buffered solution of
5NU results in a decrease on the 5NU redox peak current with a remarkable change in the peak potential (ca.
150 mV vs. Ag/AgCl) at both electrodes. This means that, an interaction of 5NU molecules with both ss- and
ds-DNA was observed. The results also demonstrate that a distinguish between ss-, and ds-DNA can be
achieved in presence of copper ion through their interaction with 5NU. The binding constants of 5NU with
ds-DNA at HMDE and GCE were determined through the changes on the 5NU redox peak currents (at
HMDE, 1.45x105 and at GCE, 2.65x105). The calibration plot for the DNA determination was obtained
through the corresponding decreases on the DPSV peak current of 5NU to different additions of DNA (ss- or
ds-DNA) concentration levels at the optimum conditions.

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Article Details

Electrochemical Comparison of the Interaction of 5-Nitrouracil with Single- or Double-Stranded DNA at mercury and glassy carbon electrodes

Authors:

Mohamed Sayed IBRAHİM | Hossieny Sameh Mohamed IBRAHİM | Moustafa Mohamed KAMAL | Yassin Mohamed TEMERK

Publisher:

Akademik Perspektif Derneği

Main Topics:

Applied physics

Journal:

Academic Platform - Journal of Engineering and Science 2014 Vol.2 Issue 1, pp.1-8

Document Type:

Article

Publication Date:

3/2014

Document Language:

English

Document ID (Org No.):

2000875247

Frequency:

Monthly

eISSN:

2822-2385

DOI:

https://doi.org/10.5505/apjes.2014.46855

Voltammetry

5-nitrouracil

interactions

DNA

Issues

Article Details

Electrochemical Comparison of the Interaction of 5-Nitrouracil with Single- or Double-Stranded DNA at mercury and glassy carbon electrodes

Authors: Mohamed Sayed IBRAHİM | Hossieny Sameh Mohamed IBRAHİM | Moustafa Mohamed KAMAL | Yassin Mohamed TEMERK

Publisher: Akademik Perspektif Derneği

Main Topics: Applied physics

Journal: Academic Platform - Journal of Engineering and Science 2014 Vol.2 Issue 1, pp.1-8

Document Type: Article

Publication Date: 3/2014

Document Language: English

Document ID (Org No.): 2000875247

Frequency: Monthly

eISSN: 2822-2385

DOI: https://doi.org/10.5505/apjes.2014.46855

Voltammetry

5-nitrouracil

interactions

DNA

Issues

Authors:

Mohamed Sayed IBRAHİM | Hossieny Sameh Mohamed IBRAHİM | Moustafa Mohamed KAMAL | Yassin Mohamed TEMERK

Publisher:

Akademik Perspektif Derneği

Main Topics:

Applied physics

Journal:

Academic Platform - Journal of Engineering and Science 2014 Vol.2 Issue 1, pp.1-8

Document Type:

Article

Publication Date:

3/2014

Document Language:

English

Document ID (Org No.):

2000875247

Frequency:

Monthly

eISSN:

2822-2385

DOI:

https://doi.org/10.5505/apjes.2014.46855