TOTAL ANTIOXIDANT STATUS, ANTIMICROBIAL AND ANTIPROLIFERATIVE POTENTIALS OF VIOLA ODORATA (FRAGRANT VIOLET)

Muhittin Doğan; Falah Saleh Mohammed; İmran Uysal; Kamil Mencik; Eylem Kına; Mustafa Pehlivan; Mustafa Sevindik

Article Details

TOTAL ANTIOXIDANT STATUS, ANTIMICROBIAL AND ANTIPROLIFERATIVE POTENTIALS OF VIOLA ODORATA (FRAGRANT VIOLET)

Authors:

Muhittin Doğan | Falah Saleh Mohammed | İmran Uysal | Kamil Mencik | Eylem Kına | Mustafa Pehlivan | Mustafa Sevindik

Publisher:

Ankara University

Main Topics:

Eczacılık ve İlaç Bilimleri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.784-791

Document Type:

Article

Publication Date:

9/2023

Document Language:

English

Document ID (Org No.):

2001291223

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status TOS and oxidative stress index OSI and total antioxidant status TAS of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected. Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line A549 was used to find out the antiproliferative effect by MTT assay. Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.7520.139, the TOS capacitiy as 7.8860.224 and the OSI capacitiy as 0.1170.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μgml intensiy and against fungi at 100-200 μgml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.

 Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status TOS and oxidative stress index OSI and total antioxidant status TAS of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected. Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line A549 was used to find out the antiproliferative effect by MTT assay. Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.7520.139, the TOS capacitiy as 7.8860.224 and the OSI capacitiy as 0.1170.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μgml intensiy and against fungi at 100-200 μgml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.

 Objective: Plants are preferred for biological effect. It is a natural resource used in the field of alternative medicine due to its biological effect. In our study, the total oxidant status TOS and oxidative stress index OSI and total antioxidant status TAS of Viola odorata L. species were detected. In addition, antimicrobial and antiproliferative effect of species was detected. Material and Method: The some parts of the species were used with the help of a soxhlet equipment, and ethanol was preferred as a solvent. TOS, OSI and TAS capacitiy were detected using Rel Assay kits. Agar dilution method was preferred to determine antimicrobial effect against bacteria and fungi. Lung cancer cell line A549 was used to find out the antiproliferative effect by MTT assay. Result and Discussion: Consequently, the studies, the TAS capacitiy of V. odorata extract was detected as 6.7520.139, the TOS capacitiy as 7.8860.224 and the OSI capacitiy as 0.1170.001. V. odorata extracts were determined to be influential against standard bacteria at 25-100 μgml intensiy and against fungi at 100-200 μgml intensiy. It was detected that the antiproliferative effect of V. odorata extract increased depending on the extract intensiy and showed strong effects. Consequently, it has been detected that V. odorata has important biological effects and in the pharmaceutical industry, it can be preferred after certain stages.

 Amaç: Bitkiler birçok biyolojik aktiviteden sorumludur. Bu kapsamda tamamlayıcı tıpta önemli doğal materyallerdir. Bu çalışmada Viola odorata L. bitkisinin toplam antioksidan durumu TAS ve toplam oksidan durumu TOS ve oksidatif stress indeksi OSI belirlenmiştir. Ayrıca bitkinin antimikrobiyal ve antiproliferatif aktivitesi tespit edilmiştir. Gereç ve Yöntem: Bitkinin toprak üstü kısımlarının etanol ile soxhlet cihazından ekstraksiyon işlemi yapılmıştır. TAS, TOS ve OSI değerleri Rel Assay kitleri kullanılarak belirlendi. Antimikrobiyal aktivite agar dilisyon metodu ile bakteri ve fungus suşlarına karşı test edilmiştir. Antiproliferatif aktivite A549 akciger kanser hücre hattına karşı MTT testi ile test edilmiştir. Sonuç ve Tartışma: Yapılan çalışmalar sonucunda bitki ekstraktının TAS değeri 6.7520.139, TOS değeri 7.8860.224 ve OSI değeri 0.1170.001 olarak belirlenmiştir. Bitki özütleri standart bakterilere karşı 25-100 μgml, funguslara karşı 100-200 μgml konsantrasyonlarda etkili olduğu görülmüştür. Bitki özütünün antiproliferative aktivitesi özüt konsantrasyonuna bağlı olarak arttığı ve güçlü etkiler gösterdiği belirlenmiştir. Sonuç olarak V. odoratanın önemli biyolojik aktivitelere sahip olduğu bu kapsamda farmakolojik ilaç dizaynlarında doğal kaynak olarak kullanılabileceği belirlenmiştir.

Article Details

TOTAL ANTIOXIDANT STATUS, ANTIMICROBIAL AND ANTIPROLIFERATIVE POTENTIALS OF VIOLA ODORATA (FRAGRANT VIOLET)

Authors:

Muhittin Doğan | Falah Saleh Mohammed | İmran Uysal | Kamil Mencik | Eylem Kına | Mustafa Pehlivan | Mustafa Sevindik

Publisher:

Ankara University

Main Topics:

Eczacılık ve İlaç Bilimleri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.784-791

Document Type:

Article

Publication Date:

9/2023

Document Language:

English

Document ID (Org No.):

2001291223

pISSN:

1015-3918

Antioxidant

fragrant violet

medicinal plants

oxidant

Viola odorataAntioksidan

kokulu menekşe

oksidan

şifalı bitkiler

Viola odorata

Issues

Article Details

Authors: Muhittin Doğan | Falah Saleh Mohammed | İmran Uysal | Kamil Mencik | Eylem Kına | Mustafa Pehlivan | Mustafa Sevindik

Publisher: Ankara University

Main Topics: Eczacılık ve İlaç Bilimleri

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.784-791

Document Type: Article

Publication Date: 9/2023

Document Language: English

Document ID (Org No.): 2001291223

pISSN: 1015-3918

Issues

Authors:

Muhittin Doğan | Falah Saleh Mohammed | İmran Uysal | Kamil Mencik | Eylem Kına | Mustafa Pehlivan | Mustafa Sevindik

Publisher:

Ankara University

Main Topics:

Eczacılık ve İlaç Bilimleri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 3, pp.784-791

Document Type:

Article

Publication Date:

9/2023

Document Language:

English

Document ID (Org No.):

2001291223

pISSN:

1015-3918