Antioxidant Activities of Propolis from Aragón (Spain)

M. Teresa SANCHO; Sandra M. OSÉS; Andrea RODRÍGUEZ-FERNÁNDEZ; Miguel A. FERNÁNDEZ-MUIÑO; Consuelo PÉREZ-ARQUILLUÉ; Regina LÁZARO; Susana BAYARRI

Article Details

Antioxidant Activities of Propolis from Aragón (Spain)

Authors:

M. Teresa SANCHO | Sandra M. OSÉS | Andrea RODRÍGUEZ-FERNÁNDEZ | Miguel A. FERNÁNDEZ-MUIÑO | Consuelo PÉREZ-ARQUILLUÉ | Regina LÁZARO | Susana BAYARRI

Publisher:

Oktay YILDIZ

Journal:

Journal of Apitherapy and Nature 2018 Vol.1 Issue 3, pp.67-67

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000492253

Frequency:

Bi-Annual

eISSN:

2667-4734

Abstract:

 


Propolis
has demonstrated a strong antioxidant activity1. This property,
together with other compositional parameters2 could contribute to
the difficult standardization of propolis. Antioxidant activity should be
determined by methods based on different mechanisms, and against several free
radicals3. The purpose of this research was to help characterize
propolis from Aragón (Spain) by determining the oxygen radical absorbance
capacity (ORAC)4, trolox equivalent antioxidant capacity (TEAC)5,
2,2-diphenyl-1-picrylhydrazyl (DPPH) assay6, as well as the
radical-scavenging effect on hydroxyl radicals (AOA)5 on
hydroalcoholic extracts of eight Aragonese propolis samples. Propolis extracts
were obtained with 70% (V/V) ethanol. All extracts showed high antioxidant
activities. The following averages were obtained: For ORAC 11070 µmol trolox/g,
for TEAC 1040.7 µmol trolox/g, for IC50 DPPH 0.0698 mg/ml and for
AOA 0.8783 mmol uric acid/g. At the 95.0% confidence level, significant linear
correlations were found between ORAC and TEAC (r= 0.9436), ORAC and AOA (r=
0.9142) and TEAC and AOA (r= 0.9919) and a double reciprocal correlation between
TEAC and DPPH (r= 0.7403). The lack of correlation between ORAC and AOA with
DPPH results is likely due to the fact that the antioxidant compounds of
Aragonese propolis could react very fast with both peroxyl (ORAC assay) and
hydroxyl (AOA assay) radicals, and at a slow pace to DPPH due to steric
inaccessibility3. Nevertheless, further studies are necessary to
corroborate this conclusion.

 Acknowledgements: To all the beekeepers who have provided with a free
sample of propolis for this study.

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Article Details

Antioxidant Activities of Propolis from Aragón (Spain)

Authors: M. Teresa SANCHO | Sandra M. OSÉS | Andrea RODRÍGUEZ-FERNÁNDEZ | Miguel A. FERNÁNDEZ-MUIÑO | Consuelo PÉREZ-ARQUILLUÉ | Regina LÁZARO | Susana BAYARRI

Publisher: Oktay YILDIZ

Journal: Journal of Apitherapy and Nature 2018 Vol.1 Issue 3, pp.67-67

Document Type: Article

Publication Date: 12/2018

Document Language: English

Document ID (Org No.): 2000492253

Frequency: Bi-Annual

eISSN: 2667-4734

Issues

Authors:

M. Teresa SANCHO | Sandra M. OSÉS | Andrea RODRÍGUEZ-FERNÁNDEZ | Miguel A. FERNÁNDEZ-MUIÑO | Consuelo PÉREZ-ARQUILLUÉ | Regina LÁZARO | Susana BAYARRI

Publisher:

Oktay YILDIZ

Journal:

Journal of Apitherapy and Nature 2018 Vol.1 Issue 3, pp.67-67

Document Type:

Article

Publication Date:

12/2018

Document Language:

English

Document ID (Org No.):

2000492253

Frequency:

Bi-Annual

eISSN:

2667-4734