Evaluation of isolated and combined effects of riluzole and sodium valproate in genetic absence epilepsy rats

Tuğba Karamahmutoğlu; Eren Şahin; Bahattin Gürbüz; Ayşe Nazlı Güneş; Ege Fakı; Rezzan Gülhan

Article Details

Evaluation of isolated and combined effects of riluzole and sodium valproate in genetic absence epilepsy rats

Authors:

Tuğba Karamahmutoğlu | Eren Şahin | Bahattin Gürbüz | Ayşe Nazlı Güneş | Ege Fakı | Rezzan Gülhan

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.38 Issue 1, pp.68-75

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001339945

pISSN:

1019-1941

Keywords:

Abstract:

 Objective: In epilepsy treatment, drugs that increase GABAergic and decrease glutamatergic activity, like sodium valproate, are used. Riluzole is a neuroprotective drug that blocks glutamatergic neurotransmission and is a potential drug with its anti-seizure effects. This study evaluates the anti-seizure effects of sodium valproate and riluzole on the genetic absence epilepsy rat from Strasbourg GAERS model individually and combined. Materials and Methods: Adult male GAERS rats n  22 were used. Rats were administered 510 mgkg riluzole, 150300 mgkg sodium valproate, 95 ethanol, and 5 mgkg riluzole combinations with 150-300 mgkg sodium valproate intraperitoneally. EEG recordings and locomotor activity tests were conducted. Statistical analysis was performed using GraphPad Prism. Results: Post-injection of 10 mgkg riluzole significantly decreased the number of spike-wave-discharges SWDs p  0.04 compared to the control group. A synergistic effect was observed with 5 mgkg riluzole and 150 mgkg sodium valproate, reducing total seizure time p  0.03 and SWDs p  0.03. Conclusion: The study demonstrated the anti-seizure effects of 150300 mgkg sodium valproate, 10 mgkg riluzole, and ethanol. A synergistic effect of 5 mgkg riluzole with 150 mgkg sodium valproate was noted. As an isolated or combined solution, riluzole shows potential, especially in resistant epilepsy treatment.

 Objective: In epilepsy treatment, drugs that increase GABAergic and decrease glutamatergic activity, like sodium valproate, are used. Riluzole is a neuroprotective drug that blocks glutamatergic neurotransmission and is a potential drug with its anti-seizure effects. This study evaluates the anti-seizure effects of sodium valproate and riluzole on the genetic absence epilepsy rat from Strasbourg GAERS model individually and combined. Materials and Methods: Adult male GAERS rats n  22 were used. Rats were administered 510 mgkg riluzole, 150300 mgkg sodium valproate, 95 ethanol, and 5 mgkg riluzole combinations with 150-300 mgkg sodium valproate intraperitoneally. EEG recordings and locomotor activity tests were conducted. Statistical analysis was performed using GraphPad Prism. Results: Post-injection of 10 mgkg riluzole significantly decreased the number of spike-wave-discharges SWDs p  0.04 compared to the control group. A synergistic effect was observed with 5 mgkg riluzole and 150 mgkg sodium valproate, reducing total seizure time p  0.03 and SWDs p  0.03. Conclusion: The study demonstrated the anti-seizure effects of 150300 mgkg sodium valproate, 10 mgkg riluzole, and ethanol. A synergistic effect of 5 mgkg riluzole with 150 mgkg sodium valproate was noted. As an isolated or combined solution, riluzole shows potential, especially in resistant epilepsy treatment.

 Objective: In epilepsy treatment, drugs that increase GABAergic and decrease glutamatergic activity, like sodium valproate, are used. Riluzole is a neuroprotective drug that blocks glutamatergic neurotransmission and is a potential drug with its anti-seizure effects. This study evaluates the anti-seizure effects of sodium valproate and riluzole on the genetic absence epilepsy rat from Strasbourg GAERS model individually and combined. Materials and Methods: Adult male GAERS rats n  22 were used. Rats were administered 510 mgkg riluzole, 150300 mgkg sodium valproate, 95 ethanol, and 5 mgkg riluzole combinations with 150-300 mgkg sodium valproate intraperitoneally. EEG recordings and locomotor activity tests were conducted. Statistical analysis was performed using GraphPad Prism. Results: Post-injection of 10 mgkg riluzole significantly decreased the number of spike-wave-discharges SWDs p  0.04 compared to the control group. A synergistic effect was observed with 5 mgkg riluzole and 150 mgkg sodium valproate, reducing total seizure time p  0.03 and SWDs p  0.03. Conclusion: The study demonstrated the anti-seizure effects of 150300 mgkg sodium valproate, 10 mgkg riluzole, and ethanol. A synergistic effect of 5 mgkg riluzole with 150 mgkg sodium valproate was noted. As an isolated or combined solution, riluzole shows potential, especially in resistant epilepsy treatment.

 Objective: In epilepsy treatment, drugs that increase GABAergic and decrease glutamatergic activity, like sodium valproate, are used. Riluzole is a neuroprotective drug that blocks glutamatergic neurotransmission and is a potential drug with its anti-seizure effects. This study evaluates the anti-seizure effects of sodium valproate and riluzole on the genetic absence epilepsy rat from Strasbourg GAERS model individually and combined. Materials and Methods: Adult male GAERS rats n  22 were used. Rats were administered 510 mgkg riluzole, 150300 mgkg sodium valproate, 95 ethanol, and 5 mgkg riluzole combinations with 150-300 mgkg sodium valproate intraperitoneally. EEG recordings and locomotor activity tests were conducted. Statistical analysis was performed using GraphPad Prism. Results: Post-injection of 10 mgkg riluzole significantly decreased the number of spike-wave-discharges SWDs p  0.04 compared to the control group. A synergistic effect was observed with 5 mgkg riluzole and 150 mgkg sodium valproate, reducing total seizure time p  0.03 and SWDs p  0.03. Conclusion: The study demonstrated the anti-seizure effects of 150300 mgkg sodium valproate, 10 mgkg riluzole, and ethanol. A synergistic effect of 5 mgkg riluzole with 150 mgkg sodium valproate was noted. As an isolated or combined solution, riluzole shows potential, especially in resistant epilepsy treatment.

Article Details

Evaluation of isolated and combined effects of riluzole and sodium valproate in genetic absence epilepsy rats

Authors:

Tuğba Karamahmutoğlu | Eren Şahin | Bahattin Gürbüz | Ayşe Nazlı Güneş | Ege Fakı | Rezzan Gülhan

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.38 Issue 1, pp.68-75

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001339945

pISSN:

1019-1941

GAERS

Riluzole

Sodium valproate

Absence epilepsy

Anti-seizure

Ethanol

Issues

Article Details

Evaluation of isolated and combined effects of riluzole and sodium valproate in genetic absence epilepsy rats

Authors: Tuğba Karamahmutoğlu | Eren Şahin | Bahattin Gürbüz | Ayşe Nazlı Güneş | Ege Fakı | Rezzan Gülhan

Publisher: Marmara University

Main Topics: Surgery (Other)

Journal: Marmara Medical Journal Vol.38 Issue 1, pp.68-75

Document Type: Article

Publication Date: 1/2025

Document Language: English

Document ID (Org No.): 2001339945

pISSN: 1019-1941

GAERS

Riluzole

Sodium valproate

Absence epilepsy

Anti-seizure

Ethanol

Issues

Authors:

Tuğba Karamahmutoğlu | Eren Şahin | Bahattin Gürbüz | Ayşe Nazlı Güneş | Ege Fakı | Rezzan Gülhan

Publisher:

Marmara University

Main Topics:

Surgery (Other)

Journal:

Marmara Medical Journal Vol.38 Issue 1, pp.68-75

Document Type:

Article

Publication Date:

1/2025

Document Language:

English

Document ID (Org No.):

2001339945

pISSN:

1019-1941