Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Aykut ÖZKUL

Article Details

Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Authors:

Aykut ÖZKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1201-1203

Document Type:

Article

Publication Date:

4/2002

Document Language:

Turkish

Document ID (Org No.):

2000770568

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Keywords:

Abstract:

 

In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.

 

In this study, the detection of canine parvovirus (CPV) in a fecal sample from a dog with enteritis was performed for the first time using the polymerase chain reaction (PCR) in Turkey. The final PCR product was analyzed using the restriction fragment length polymorphysm (RFLP) technique. RFLP analysis using Apa LI and Eco RV restriction endonucleases revealed homology in the nucleotide sequence in at least the VP2 coding region of the virus DNAs detected in the fecal specimen and prepared from attenuated vaccine virus as a positive control.

Article Details

Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Authors:

Aykut ÖZKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1201-1203

Document Type:

Article

Publication Date:

4/2002

Document Language:

Turkish

Document ID (Org No.):

2000770568

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Canine parvovirus

diagnosis

PCR

RFLP

Issues

Article Details

Detection and RFLP Analysis of Canine Parvovirus (CPV) DNA by Polymerase Chain Reaction (PCR) in a Dog

Authors: Aykut ÖZKUL

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1201-1203

Document Type: Article

Publication Date: 4/2002

Document Language: Turkish

Document ID (Org No.): 2000770568

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Canine parvovirus

diagnosis

PCR

RFLP

Issues

Authors:

Aykut ÖZKUL

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2002 Vol.26 Issue 5, pp.1201-1203

Document Type:

Article

Publication Date:

4/2002

Document Language:

Turkish

Document ID (Org No.):

2000770568

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128