Isolation and Partial Purifiction of Arginase from Sera of Women with Uterine Fibroids

Taghreed U. Mohammd; Eiman A. Abass; Manar A. Salman

Article Details

Isolation and Partial Purifiction of Arginase from Sera of Women with Uterine Fibroids

Authors:

Taghreed U. Mohammd | Eiman A. Abass | Manar A. Salman

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.28 Issue 2, pp.116-125

Document Type:

Article

Publication Date:

1/2015

Document Language:

English

Document ID (Org No.):

2001377325

pISSN:

2521-3407

Keywords:

Abstract:

 The first aim of the present study was performed to assay the activity of arginase in sera of women with uterine fibroid..   This study consisted of(50) women with uterine fibroid as patient's group  and (30) healthy women as control group. The age ranged between (30-55) years for the two groups. The results showed that highly significant increas (P< 0.0001) in the arginase activity in sera of women with uterine fibroid (7.99Â± 0.23) I.U/L is found when compared with healthy group (0.52Â±0.02) I.U/L. The second aim was performed to isolate arginase from sera of women with uterine fibroids. The purification is done by addition of ammonium sulfate, dialysis, gel filtration chromatography by using sephadex G-50 and ion exchange chromatography by using DEAE cellulose A-50. The results showed a single band for isoenzymes following steps by using sodium dodecyl sulfate- polyacryl amide gel electrophoresis (SDS-PAGE) .

Article Details

Isolation and Partial Purifiction of Arginase from Sera of Women with Uterine Fibroids

Authors:

Taghreed U. Mohammd | Eiman A. Abass | Manar A. Salman

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.28 Issue 2, pp.116-125

Document Type:

Article

Publication Date:

1/2015

Document Language:

English

Document ID (Org No.):

2001377325

pISSN:

2521-3407

Arginase

Uterine fibroid

Issues

Article Details

Isolation and Partial Purifiction of Arginase from Sera of Women with Uterine Fibroids

Authors: Taghreed U. Mohammd | Eiman A. Abass | Manar A. Salman

Publisher: University of Baghdad

Journal: Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.28 Issue 2, pp.116-125

Document Type: Article

Publication Date: 1/2015

Document Language: English

Document ID (Org No.): 2001377325

pISSN: 2521-3407

Arginase

Uterine fibroid

Issues

Authors:

Taghreed U. Mohammd | Eiman A. Abass | Manar A. Salman

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.28 Issue 2, pp.116-125

Document Type:

Article

Publication Date:

1/2015

Document Language:

English

Document ID (Org No.):

2001377325

pISSN:

2521-3407