Determination of polymyxin B, minocycline, colistin and phosphomycin susceptibilities in Acinetobacter baumannii strains showing carbapenem resistant multidrug resistance phenotype

Salih CESUR; Sami KINIKLI; Salih CESUR; Mihriban YÜCEL; Çiğdem ATAMAN HATİPOĞLU; Bedia DİNÇ

doi:https://doi.org/10.32322/jhsm.456990

Article Details

Determination of polymyxin B, minocycline, colistin and phosphomycin susceptibilities in Acinetobacter baumannii strains showing carbapenem resistant multidrug resistance phenotype

Authors:

Salih CESUR | Sami KINIKLI | Salih CESUR | Mihriban YÜCEL | Çiğdem ATAMAN HATİPOĞLU | Bedia DİNÇ

Publisher:

MediHealth Academy Yayıncılık

Main Topics:

Promotion of health

Journal:

Journal of Health Sciences and Medicine 2019 Vol.2 Issue 2, pp.49-53

Document Type:

Article

Publication Date:

3/2019

Document Language:

English

Document ID (Org No.):

2000482485

Frequency:

Bimonthly

eISSN:

2636-8579

DOI:

https://doi.org/10.32322/jhsm.456990

Abstract:

 


Objective: In this study, the sensitivity of minocycline, polymyxin B, cholestin and phosphomycin to carbapenem resistant multiresistant Acinetobacter baumannii (A. baumannii) strains (resistant to three or more antibiotic groups) isolated from patients in Ankara Training and Research Hospital It was aimed.


Material and Method: Eighty nosocomial A. baumanii strains with a carbapenem resistant multidrug resistance phenotype were included in the study. Sensitivities of minocycline, polymyxin B and phosphomycin were determined by disc diffusion test and colistin susceptibility test by disk diffusion and E-test methods.


Results: Eighty (100%) polymyxin B, 75 (93,75%) minocycline sensitive and 4 (5%) medium susceptible to 80 carbapenem resistant A.baumannii strains were evaluated by disc diffusion method. 75 (93.5%) were sensitive to colistin, 5 (6.25%) were moderately sensitive to colistin and 2 (2.5%) were sensitive to phosphomycin. By e-test, all 80 strains (100%) were detected as susceptible to colistin. The MIC range for colistin was 0.125-1.5 μg / ml, the MIC 50 value was 025 μg / ml (the MIC range was found to be 0.5 μg / ml).









Conclusion: Polymyxin B and minocycline could be used in therapy because phosphomycin B and susceptibility rates were high in empirical treatment of carbamazepine resistant multispecific A.baumannii strains in our hospital. Phosphomycin could not be used in treatment because of high resistance rate.



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Amaç: Bu çalışmada, Ankara Eğitim ve
Araştırma Hastanesi’nde yatan hastalardan izole edilen, karbapeneme dirençli,
çoklu ilaca dirençli (üç ve daha fazla antibiyotik grubuna dirençli) Acinetobacter baumannii (A. baumannii) suşlarının minosiklin,
polimiksin B, kolistin ve fosfomisin duyarlılıklarının belirlenmesi amaçlandı.


Gereç ve Yöntem: Çalışmaya karbapeneme dirençli,
çoklu ilaç direnci fenotipi gösteren 80 nozokomiyal kökenli A. baumanii
suşu dahil edildi. Suşların minosiklin, polimiksin B ve fosfomisin duyarlılıkları
disk difüzyon testiyle, kolistin duyarlılıkları ise  disk difüzyon ve E-test yöntemleriyle
belirlendi.


Bulgular:
Çalışmaya alınan toplam 80 karbapeneme dirençli A.baumannii suşunun disk difüzyon yöntemi ile  80 (%100)’nin polimiksin B’ye, 75 (%93,75)’inin
minosikline duyarlı, 4 (%5)’inin orta duyarlı, 75 (%93.5)’inin  kolistine duyarlı, 5 (%6,25)’sının  kolistine orta duyarlı, 2 (%2,5)’sinin ise   fosfomisine duyarlı olduğu belirlendi.    E-test yöntemiyle 80 suşun tamamı (%100)
kolistine duyarlı olarak saptandı. Kolistin için MİK aralığı 0,125-1.5 μg/ml,
MİK 50 değeri 025 μg/ml (MİK aralığı iken, MİK90 değeri 0,5 μg/ml olarak
belirlendi. 









Sonuç: Hastanemizde karbapeneme
direçli, çoklu ilaca dirençli A.baumannii
suşlarının ampirik tedavisinde duyarlılık oranlarının yüksek olması nedeniyle
polimiksin B ve minosiklinin tedavide kullanılabileği, fosfomisinin ise direnç
oranının yüksek olması nedeniyle tedavide kullanılamayacağı belirlendi.



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Article Details

Determination of polymyxin B, minocycline, colistin and phosphomycin susceptibilities in Acinetobacter baumannii strains showing carbapenem resistant multidrug resistance phenotype

Authors: Salih CESUR | Sami KINIKLI | Salih CESUR | Mihriban YÜCEL | Çiğdem ATAMAN HATİPOĞLU | Bedia DİNÇ

Publisher: MediHealth Academy Yayıncılık

Main Topics: Promotion of health

Journal: Journal of Health Sciences and Medicine 2019 Vol.2 Issue 2, pp.49-53

Document Type: Article

Publication Date: 3/2019

Document Language: English

Document ID (Org No.): 2000482485

Frequency: Bimonthly

eISSN: 2636-8579

DOI: https://doi.org/10.32322/jhsm.456990

Issues

Authors:

Salih CESUR | Sami KINIKLI | Salih CESUR | Mihriban YÜCEL | Çiğdem ATAMAN HATİPOĞLU | Bedia DİNÇ

Publisher:

MediHealth Academy Yayıncılık

Main Topics:

Promotion of health

Journal:

Journal of Health Sciences and Medicine 2019 Vol.2 Issue 2, pp.49-53

Document Type:

Article

Publication Date:

3/2019

Document Language:

English

Document ID (Org No.):

2000482485

Frequency:

Bimonthly

eISSN:

2636-8579

DOI:

https://doi.org/10.32322/jhsm.456990