Evaluation of Free Radical and Antioxidant Enzyme Levels in Patients with COVID-19 Possible/Definitive Diagnosis

Fatih Davran; Ceyhan Hacıoğlu; Merve Alpay; Pınar Yıldız Gülhan; Cihadiye Elif Öztürk; Nevin Ince; Elif Nisa Unlu; Ali Annakkaya

Article Details

Evaluation of Free Radical and Antioxidant Enzyme Levels in Patients with COVID-19 Possible/Definitive Diagnosis

Authors:

Fatih Davran | Ceyhan Hacıoğlu | Merve Alpay | Pınar Yıldız Gülhan | Cihadiye Elif Öztürk | Nevin Ince | Elif Nisa Unlu | Ali Annakkaya

Publisher:

Duzce University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Sağlık Bilimlerinde Değer Vol.13 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281925

pISSN:

2792-0542

Keywords:

Abstract:

 Aim: Although coronavirus disease 2019 COVID-19 is a complex disease threatening global public health and leading to a pandemic, the contributions of the pro-oxidantoxidant imbalance involved in the pathogenesis of the disease have not been adequately studied. Therefore, the aim of this study is to evaluate the effects of smoking on oxidative stress and antioxidant defense system biomarkers in COVID-19 patients. Material and Methods: Participants were divided into 4 groups in the study: Control group 45 healthy individuals without a diagnosis of COVID-19 and not smoking, Group 2 45 individuals without a diagnosis of COVID-19 and smoking, Group 3 45 patients with a diagnosis of COVID-19 and not smoking, Group 4 45 patients with a diagnosis of COVID-19 and smoking 45 patients. D-dimer, nitric oxide NO, glutathione reductase GR, superoxide dismutase SOD, catalase CAT, malondialdehyde MDA, total antioxidant levels TAS and total oxidant levels TOS in the serum of the participants were analyzed using commercial kits according to the ELISA method. Results: There is a significant increase in D-dimer levels in people diagnosed with COVID-19 p0.001. TAS, CAT, SOD and GR levels in Group II, Group III and Group IV showed a statistical decrease when compared to the control group. Compared to the control group, there was a significant increase in TOS and MDA levels, especially in Group IV p0.001. In addition, NO levels in Group II, Group III and Group IV were significantly increased compared to the control group p0.001. Conclusion: Our data show that smoking increases oxidative and nitrosative damage in COVID-19.

 Aim: Although coronavirus disease 2019 COVID-19 is a complex disease threatening global public health and leading to a pandemic, the contributions of the pro-oxidantoxidant imbalance involved in the pathogenesis of the disease have not been adequately studied. Therefore, the aim of this study is to evaluate the effects of smoking on oxidative stress and antioxidant defense system biomarkers in COVID-19 patients. Material and Methods: Participants were divided into 4 groups in the study: Control group 45 healthy individuals without a diagnosis of COVID-19 and not smoking, Group 2 45 individuals without a diagnosis of COVID-19 and smoking, Group 3 45 patients with a diagnosis of COVID-19 and not smoking, Group 4 45 patients with a diagnosis of COVID-19 and smoking 45 patients. D-dimer, nitric oxide NO, glutathione reductase GR, superoxide dismutase SOD, catalase CAT, malondialdehyde MDA, total antioxidant levels TAS and total oxidant levels TOS in the serum of the participants were analyzed using commercial kits according to the ELISA method. Results: There is a significant increase in D-dimer levels in people diagnosed with COVID-19 p0.001. TAS, CAT, SOD and GR levels in Group II, Group III and Group IV showed a statistical decrease when compared to the control group. Compared to the control group, there was a significant increase in TOS and MDA levels, especially in Group IV p0.001. In addition, NO levels in Group II, Group III and Group IV were significantly increased compared to the control group p0.001. Conclusion: Our data show that smoking increases oxidative and nitrosative damage in COVID-19.

 Aim: Although coronavirus disease 2019 COVID-19 is a complex disease threatening global public health and leading to a pandemic, the contributions of the pro-oxidantoxidant imbalance involved in the pathogenesis of the disease have not been adequately studied. Therefore, the aim of this study is to evaluate the effects of smoking on oxidative stress and antioxidant defense system biomarkers in COVID-19 patients. Material and Methods: Participants were divided into 4 groups in the study: Control group 45 healthy individuals without a diagnosis of COVID-19 and not smoking, Group 2 45 individuals without a diagnosis of COVID-19 and smoking, Group 3 45 patients with a diagnosis of COVID-19 and not smoking, Group 4 45 patients with a diagnosis of COVID-19 and smoking 45 patients. D-dimer, nitric oxide NO, glutathione reductase GR, superoxide dismutase SOD, catalase CAT, malondialdehyde MDA, total antioxidant levels TAS and total oxidant levels TOS in the serum of the participants were analyzed using commercial kits according to the ELISA method. Results: There is a significant increase in D-dimer levels in people diagnosed with COVID-19 p0.001. TAS, CAT, SOD and GR levels in Group II, Group III and Group IV showed a statistical decrease when compared to the control group. Compared to the control group, there was a significant increase in TOS and MDA levels, especially in Group IV p0.001. In addition, NO levels in Group II, Group III and Group IV were significantly increased compared to the control group p0.001. Conclusion: Our data show that smoking increases oxidative and nitrosative damage in COVID-19.

 Amaç: Koronavirüs hastalığı 2019 COVID-19, küresel halk sağlığını tehdit eden ve bir pandemiye yol açan karmaşık bir hastalık olmasına rağmen, hastalığın patogenezinde yer alan pro-oksidanoksidan dengesizliğinin katkıları yeterince araştırılmamıştır. Bu nedenle, bu çalışmanın amacı, COVID-19 hastalarında sigara kullanımının oksidatif stres ve antioksidan savunma sistemi biyobelirteçleri üzerindeki etkilerini değerlendirmektir. Gereç ve Yöntemler: Çalışmaya katılan kişiler 4 gruba ayrıldı: Kontrol grubu COVID-19 tanısı olmayan ve sigara kullanmayan 45 sağlıklı birey, Grup 2 COVID-19 tanısı olmayan ve sigara kullanan 45 birey, Grup 3 COVID-19 tanılı ve sigara kullanmayan 45 hasta, Grup 4 COVID-19 tanılı ve sigara kullanan 45 hasta. Katılımcıların serumlarındaki D-dimer, nitrik oksit NO, glutatyon redüktaz GR, süperoksit dismutaz SOD, katalaz CAT, malondialdehid MDA, total antioksidan seviyeleri TAS ve total oksidan seviyeleri TOS seviyelerini ticari kitler kullanarak ELİSA yöntemine göre analiz edildi. Bulgular: COVID-19 tanısı alan kişilerdeki D-dimer seviyelerinde anlamlı bir artış söz konusudur p0,001. Grup II, Grup III ve Grup IVdeki TAS, CAT, SOD ve GR seviyeleri kontrol grubu ile karşılaştırıldığında istatiksel olarak bir düşüş göstermiştir. Kontrol grubu ile karşılaştırıldığında özellikle de Grup IVdeki TOS ve MDA seviyelerinde ciddi artış olmuştur p0,001. Ek olarak, Grup II, Grup III ve Grup IVdeki NO seviyeleri kontrol grubuna göre anlamlı bir şekilde artmıştır p0,001. Sonuç: Verilerimiz, sigara kullanımının COVID-19daki oksidatif ve nitrozatif hasarı arttırdığı göstermektedir.

Article Details

Evaluation of Free Radical and Antioxidant Enzyme Levels in Patients with COVID-19 Possible/Definitive Diagnosis

Authors:

Fatih Davran | Ceyhan Hacıoğlu | Merve Alpay | Pınar Yıldız Gülhan | Cihadiye Elif Öztürk | Nevin Ince | Elif Nisa Unlu | Ali Annakkaya

Publisher:

Duzce University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Sağlık Bilimlerinde Değer Vol.13 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281925

pISSN:

2792-0542

COVID-19

smoking

oxidative stress

nitrosative stress

COVID-19

sigara kullanımı

oksidatif stres

nitrozatif stres

Issues

Article Details

Authors: Fatih Davran | Ceyhan Hacıoğlu | Merve Alpay | Pınar Yıldız Gülhan | Cihadiye Elif Öztürk | Nevin Ince | Elif Nisa Unlu | Ali Annakkaya

Publisher: Duzce University

Main Topics: Klinik Tıp Bilimleri

Journal: Sağlık Bilimlerinde Değer Vol.13 Issue 1, pp.93-98

Document Type: Article

Publication Date: 1/2023

Document Language: Turkish

Document ID (Org No.): 2001281925

pISSN: 2792-0542

Issues

Authors:

Fatih Davran | Ceyhan Hacıoğlu | Merve Alpay | Pınar Yıldız Gülhan | Cihadiye Elif Öztürk | Nevin Ince | Elif Nisa Unlu | Ali Annakkaya

Publisher:

Duzce University

Main Topics:

Klinik Tıp Bilimleri

Journal:

Sağlık Bilimlerinde Değer Vol.13 Issue 1, pp.93-98

Document Type:

Article

Publication Date:

1/2023

Document Language:

Turkish

Document ID (Org No.):

2001281925

pISSN:

2792-0542