Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus  for improved serotype detection

Waqas ALI; Mudasser HABIB; Muhammad SALAH UD DIN SHAH

Article Details

Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection

Authors:

Waqas ALI | Mudasser HABIB | Muhammad SALAH UD DIN SHAH

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.335-345

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000764615

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128

Abstract:

 

 
                         Frequently, a sequence from a single strain of a virus is used to design primers for PCR-based virus detection. However, high 
mutation rates in RNA viruses lead to failure of microorganism detection in clinical samples. Therefore, it is essential to find conserved 
regions within sequences to design primers for the detection of pathogen genomes in suspected clinical samples. The aim of the study 
was to find conserved regions of the genome for improved serotype detection. In the present study, primers were designed for serotype 
detection based on the VP2 coding region of foot-and-mouth disease virus (FMDV) after alignment of full genome (n = 375) sequences. 
These primer pairs and previously reported primer pairs were then compared using positive samples (n = 91) collected during 2010?
2016 from Faisalabad District, Pakistan. The detection rate of newly designed primer pairs O-Wqs-F/Rev-Wqs (96.5%), As-Wqs-F/Rev-
Wqs (90.4%), and A-Wqs-F/Rev-Wqs (100%) was better compared with previously reported primer pairs P38/P33 (81%), P74?77/P33 
(47.6%), and P87?92/P33 (41.6%) in detecting FMDV serotypes O, Asia1, and A, respectively. The higher detection rates of the newly 
designed primer pairs appeared to be due to the selection of highly conserved sequences within the serotypes for designing primers. 
To the best of the authors? knowledge, this is the first study that describes serotype diagnosis of FMDV based on primers targeting the 
VP2 region. In conclusion, this new method offers an improved approach for the serotyping of FMDV compared to previous methods.

Article Details

Evaluation of PCR primers targeting the VP2 region of the foot-and-mouth disease virus for improved serotype detection

Authors: Waqas ALI | Mudasser HABIB | Muhammad SALAH UD DIN SHAH

Publisher: TUBITAK

Journal: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.335-345

Document Type: Article

Publication Date: 8/2018

Document Language: English

Document ID (Org No.): 2000764615

Frequency: Bimonthly

eISSN: 1303-6181

pISSN: 1300-0128

Issues

Authors:

Waqas ALI | Mudasser HABIB | Muhammad SALAH UD DIN SHAH

Publisher:

TUBITAK

Journal:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.335-345

Document Type:

Article

Publication Date:

8/2018

Document Language:

English

Document ID (Org No.):

2000764615

Frequency:

Bimonthly

eISSN:

1303-6181

pISSN:

1300-0128