Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa

Nidhal N. Hussain; Ghaith M. Fadhil

Article Details

Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa

Authors:

Nidhal N. Hussain | Ghaith M. Fadhil

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.29 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

1/2016

Document Language:

English

Document ID (Org No.):

2001377403

pISSN:

2521-3407

Keywords:

Abstract:

 The cellular and molecular toxicity effect  of Aloe Vera crude extract on meristem cells of onion  (Allium cepa)  roots were studied  . Different concentrations of this crude extract   20% ,10% ,5%   ,2% ( and 40%) were used at different periods ; 24 ,48 and 72 hour . Number of  chromosomal aberrations and the mitotic index was calculated . In addition, the genetic effects were observed by  using randomly amplified polymorphic DNA ( RAPD ) technique . The results  showed that the gel crud extract of Aloe vera  had inhibition effects on the growth of the onion roots with 10% EC50  value. Significant effects of this gel crud extract were also observed on the chromosomal aberration and mitotic index by decreasing of divided cell rates. The toxic effect of 20% concentration of gel crud extract was decreased the mitotic index under 50% , in the comparison with control sample . Chromosomal aberration were also observed in divided cells of onion roots, which exposed to different concentrations at different periods . The higher frequent type of this chromosomal aberrations , which observed  are stickiness , disturbed , bridge , c-mitosis , vagrant and star telophase . The result of RAPD technique  reveled differences in number of DNA bands and their molecular weights of the treated samples , which exposed to high concentration of crud extract in comparison with the control sample . The result of the genetic stability ratio (% GTS), also showed that Aloe vera crud extract have genetic toxicity at 40% concentration.

Article Details

Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa

Authors:

Nidhal N. Hussain | Ghaith M. Fadhil

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.29 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

1/2016

Document Language:

English

Document ID (Org No.):

2001377403

pISSN:

2521-3407

genotoxicity

Allium cepa

RAPD

Aloe vera

Issues

Article Details

Detection of Cellular and Molecular Toxicity of Aloe vera Gel Crude Extract on the Roots of the Allium cepa

Authors: Nidhal N. Hussain | Ghaith M. Fadhil

Publisher: University of Baghdad

Journal: Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.29 Issue 1, pp.0-0

Document Type: Article

Publication Date: 1/2016

Document Language: English

Document ID (Org No.): 2001377403

pISSN: 2521-3407

genotoxicity

Allium cepa

RAPD

Aloe vera

Issues

Authors:

Nidhal N. Hussain | Ghaith M. Fadhil

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.29 Issue 1, pp.0-0

Document Type:

Article

Publication Date:

1/2016

Document Language:

English

Document ID (Org No.):

2001377403

pISSN:

2521-3407