Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Muaz BELVİRANLI; Hakki GÖKBEL; Nilsel OKUDAN; Sadik BÜYÜKBAŞ

Article Details

Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Authors:

Muaz BELVİRANLI | Hakki GÖKBEL | Nilsel OKUDAN | Sadik BÜYÜKBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.489-495

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752641

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

Background/aim: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats.   Materials and methods: Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg-1 day-1) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed.  Results: Both XO and ADA activities increased and NO levels decreased in diabetic rats (P < 0.05). GSE supplementation normalized all of these changes. Antioxidant enzyme activities decreased in diabetic rats compared to the controls (P < 0.05). GSE supplementation increased antioxidant enzyme activities in both diabetic and healthy rats (P < 0.05).   Conclusion: These findings suggest that 6 weeks of oral GSE supplementation may prevent oxidative stress and improve antioxidant status in diabetic rats.

 

Background/aim: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats.   Materials and methods: Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg-1 day-1) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed.  Results: Both XO and ADA activities increased and NO levels decreased in diabetic rats (P < 0.05). GSE supplementation normalized all of these changes. Antioxidant enzyme activities decreased in diabetic rats compared to the controls (P < 0.05). GSE supplementation increased antioxidant enzyme activities in both diabetic and healthy rats (P < 0.05).   Conclusion: These findings suggest that 6 weeks of oral GSE supplementation may prevent oxidative stress and improve antioxidant status in diabetic rats.

Article Details

Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Authors:

Muaz BELVİRANLI | Hakki GÖKBEL | Nilsel OKUDAN | Sadik BÜYÜKBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.489-495

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752641

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Experimental diabetes

grape seed extract

oxidative stress

antioxidant enzyme activity

adenosine deaminase

nitric oxide

Issues

Article Details

Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Authors: Muaz BELVİRANLI | Hakki GÖKBEL | Nilsel OKUDAN | Sadik BÜYÜKBAŞ

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.489-495

Document Type: Article

Publication Date: 8/2015

Document Language: Turkish

Document ID (Org No.): 2000752641

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Experimental diabetes

grape seed extract

oxidative stress

antioxidant enzyme activity

adenosine deaminase

nitric oxide

Issues

Authors:

Muaz BELVİRANLI | Hakki GÖKBEL | Nilsel OKUDAN | Sadik BÜYÜKBAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2015 Vol.45 Issue 3, pp.489-495

Document Type:

Article

Publication Date:

8/2015

Document Language:

Turkish

Document ID (Org No.):

2000752641

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144