Micropropagation of Erodium sibthorpianum subsp. sibthorpianum, an endemic threatened species of Uludağ Mountain (Bursa-Turkey)

Betül AKIN; İsmail KOCAÇALIŞKAN; Gürcan GÜLERYÜZ

Article Details

Micropropagation of Erodium sibthorpianum subsp. sibthorpianum, an endemic threatened species of Uludağ Mountain (Bursa-Turkey)

Authors:

Betül AKIN | İsmail KOCAÇALIŞKAN | Gürcan GÜLERYÜZ

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2014 Vol.38 Issue 1, pp.148-155

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000722509

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X

Abstract:

 

Uludağ Mountain is one of the Important Plant Areas of Turkey. Its native plant species are threatened by various human and environmental factors. In this study, 2 different micropropagation techniques, with Murashige Skoog (MS) and modified MS (ModMS) media, were used for Erodium sibthorpianum Boiss. subsp. sibthorpianum. Mineral composition was developed according to the plant requirements. Shoot tips were cultured on these media. Seedlings, obtained from in vitro germinated seeds, were used as explant sources in micropropagation studies. The highest percentage of shoot induction (11.33 shoots/explant) was determined in 1 ppm 6-benzyl amino purine and plant growth regulator-free MS medium (35.97 mm shoot length). Elongated shoots were successfully rooted in the ModMS medium with different naphthalene acetic acid concentrations. Shoots with regenerated roots were transferred to the greenhouse. This protocol could be used for conservation studies of endemic and endangered plant species.

 

Uludağ Mountain is one of the Important Plant Areas of Turkey. Its native plant species are threatened by various human and environmental factors. In this study, 2 different micropropagation techniques, with Murashige Skoog (MS) and modified MS (ModMS) media, were used for Erodium sibthorpianum Boiss. subsp. sibthorpianum. Mineral composition was developed according to the plant requirements. Shoot tips were cultured on these media. Seedlings, obtained from in vitro germinated seeds, were used as explant sources in micropropagation studies. The highest percentage of shoot induction (11.33 shoots/explant) was determined in 1 ppm 6-benzyl amino purine and plant growth regulator-free MS medium (35.97 mm shoot length). Elongated shoots were successfully rooted in the ModMS medium with different naphthalene acetic acid concentrations. Shoots with regenerated roots were transferred to the greenhouse. This protocol could be used for conservation studies of endemic and endangered plant species.

Article Details

Micropropagation of Erodium sibthorpianum subsp. sibthorpianum, an endemic threatened species of Uludağ Mountain (Bursa-Turkey)

Authors: Betül AKIN | İsmail KOCAÇALIŞKAN | Gürcan GÜLERYÜZ

Publisher: TUBITAK

Journal: Turkish Journal of Botany 2014 Vol.38 Issue 1, pp.148-155

Document Type: Article

Publication Date: 5/2014

Document Language: Turkish

Document ID (Org No.): 2000722509

Frequency: Bimonthly

eISSN: 1303-6106

pISSN: 1300-008X

Issues

Authors:

Betül AKIN | İsmail KOCAÇALIŞKAN | Gürcan GÜLERYÜZ

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2014 Vol.38 Issue 1, pp.148-155

Document Type:

Article

Publication Date:

5/2014

Document Language:

Turkish

Document ID (Org No.):

2000722509

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X