Effects of Quercetin on Cypermethrin-Induced Stomach Injury: The Role of Oxidative Stress, Inflammation, and Apoptosis.

Nurhan Akaras; Cihan Gür; Hasan Şimşek; Sibel Çiğdem Tuncer

Article Details

Effects of Quercetin on Cypermethrin-Induced Stomach Injury: The Role of Oxidative Stress, Inflammation, and Apoptosis.

Authors:

Nurhan Akaras | Cihan Gür | Hasan Şimşek | Sibel Çiğdem Tuncer

Publisher:

Gumushane University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 2, pp.556-566

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001291796

pISSN:

2146-9954

Keywords:

Abstract:

 This study was conducted to investigate the effects of quercetin QUE on cypermethrin CYP induced gastrotoxicity in rats. 35 Sprague-Dawley rats were randomly divided into five groups, 7 in each group. In the study, 25 and 50 mgkg QUE were administered orally 30 min after 25 mgkg cypermethrin was administered to rats for 28 days. Oxidative stress, inflammation, ER stress, apoptosis and autophagy markers were biochemically analyzed in gastric tissues. Additionally, histological analysis was performed for microscopic evaluation of gastric tissue. The results revealed that QUE prevented tissue damage by reducing CYP-induced lipid peroxidation MDA and increasing GSH, SOD, CAT and GPx activities. It also showed anti-inflammatory effect by suppressing inflammatory markers such as NF-B, IL-1β, TNF-α, iNOS and COX-2. QUE administration down-regulated CYP-induced increased PERK, ATF6, Caspase-3 and Beclin-1 markers. In addition, administration of QUE ameliorated the pathological tissue damage in gastric tissue due to CYP. The data of this study show that Que suppresses CYP-induced gastric toxicity by reducing oxidative stress, inflammation, ER stress, apoptosis a autophagy.

 This study was conducted to investigate the effects of quercetin QUE on cypermethrin CYP induced gastrotoxicity in rats. 35 Sprague-Dawley rats were randomly divided into five groups, 7 in each group. In the study, 25 and 50 mgkg QUE were administered orally 30 min after 25 mgkg cypermethrin was administered to rats for 28 days. Oxidative stress, inflammation, ER stress, apoptosis and autophagy markers were biochemically analyzed in gastric tissues. Additionally, histological analysis was performed for microscopic evaluation of gastric tissue. The results revealed that QUE prevented tissue damage by reducing CYP-induced lipid peroxidation MDA and increasing GSH, SOD, CAT and GPx activities. It also showed anti-inflammatory effect by suppressing inflammatory markers such as NF-B, IL-1β, TNF-α, iNOS and COX-2. QUE administration down-regulated CYP-induced increased PERK, ATF6, Caspase-3 and Beclin-1 markers. In addition, administration of QUE ameliorated the pathological tissue damage in gastric tissue due to CYP. The data of this study show that Que suppresses CYP-induced gastric toxicity by reducing oxidative stress, inflammation, ER stress, apoptosis a autophagy.

 This study was conducted to investigate the effects of quercetin QUE on cypermethrin CYP induced gastrotoxicity in rats. 35 Sprague-Dawley rats were randomly divided into five groups, 7 in each group. In the study, 25 and 50 mgkg QUE were administered orally 30 min after 25 mgkg cypermethrin was administered to rats for 28 days. Oxidative stress, inflammation, ER stress, apoptosis and autophagy markers were biochemically analyzed in gastric tissues. Additionally, histological analysis was performed for microscopic evaluation of gastric tissue. The results revealed that QUE prevented tissue damage by reducing CYP-induced lipid peroxidation MDA and increasing GSH, SOD, CAT and GPx activities. It also showed anti-inflammatory effect by suppressing inflammatory markers such as NF-B, IL-1β, TNF-α, iNOS and COX-2. QUE administration down-regulated CYP-induced increased PERK, ATF6, Caspase-3 and Beclin-1 markers. In addition, administration of QUE ameliorated the pathological tissue damage in gastric tissue due to CYP. The data of this study show that Que suppresses CYP-induced gastric toxicity by reducing oxidative stress, inflammation, ER stress, apoptosis a autophagy.

 Bu çalışma, ratlarda sipermetrin CYP kaynaklı gastrotoksisite üzerine kuersetinin QUE etkilerini araştırmak için yapıldı. 35 adet Sprague Dawley ratı, her grupta 7 tane olacak şekilde rastgele beş gruba ayrıldı. Çalışmada, ratlara 28 gün boyunca 25 mgkg sipermetrin uygulandıktan 30 dakika sonra oral olarak 25 ve 50 mgkg QUE verildi. Mide dokularında oksidatif stres, inflamasyon, ER stres, apoptoz ve otofaji belirteçleri biyokimyasal olarak analiz edildi. Ayrıca mide dokusu mikroskobik değerlendirme için histolojik analiz yapıldı. Sonuçlar, QUEnin CYP kaynaklı lipit peroksidasyonunu MDA düşürerek ve GSH, SOD, KAT ve GPx aktivitelerini artırarak doku hasarını önlediğini ortaya koydu. Ayrıca NF-B, IL-1β, TNF-α, iNOS ve COX-2 gibi inflamatuar belirteçleri baskılayarak anti-inflamatuar etki gösterdi. CYP kaynaklı artan PERK, ATF6, Kaspaz-3 ve Beklin-1 belirteçlerini QUE uygulanması aşağı regüle etti. Ek olarak, CYPye bağlı olarak oluşan mide dokusundaki patolojik doku hasarını QUE verilmesi iyileştirdi. Bu çalışmanın verileri Que nin oksidatif stresi, enflamasyonu, ER stresi, apoptozu ve otofajiyi iyileştirerek CYP kaynaklı mide toksisitesini baskıladığını göstermektedir.

Article Details

Effects of Quercetin on Cypermethrin-Induced Stomach Injury: The Role of Oxidative Stress, Inflammation, and Apoptosis.

Authors:

Nurhan Akaras | Cihan Gür | Hasan Şimşek | Sibel Çiğdem Tuncer

Publisher:

Gumushane University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 2, pp.556-566

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001291796

pISSN:

2146-9954

Cypermethrin

Gastrotoxicity

Oxidative Stress

Quercetin

RatGastrotoksisite

Kuersetin

Oksidatif Stres

Rat

Sipermetrin

Issues

Article Details

Authors: Nurhan Akaras | Cihan Gür | Hasan Şimşek | Sibel Çiğdem Tuncer

Publisher: Gumushane University

Main Topics: Sağlık Kurumları Yönetimi

Journal: Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 2, pp.556-566

Document Type: Article

Publication Date: 6/2023

Document Language: English

Document ID (Org No.): 2001291796

pISSN: 2146-9954

Issues

Authors:

Nurhan Akaras | Cihan Gür | Hasan Şimşek | Sibel Çiğdem Tuncer

Publisher:

Gumushane University

Main Topics:

Sağlık Kurumları Yönetimi

Journal:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.12 Issue 2, pp.556-566

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001291796

pISSN:

2146-9954