Preliminary Studies on Gynostemma pentaphyllum (Thunb.) Makino toward Dietary Supplement Development: Antidiabetic Activity and Phytochemical Analysis

Methiye Mancak; Fatma Zeynep Uzuner; Ufuk Koca

Article Details

Preliminary Studies on Gynostemma pentaphyllum (Thunb.) Makino toward Dietary Supplement Development: Antidiabetic Activity and Phytochemical Analysis

Authors:

Methiye Mancak | Fatma Zeynep Uzuner | Ufuk Koca

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Journal:

Karaelmas Fen ve Mühendislik Dergisi Vol.15 Issue 2, pp.15-21

Document Type:

Article

Publication Date:

7/2025

Document Language:

Turkish

Document ID (Org No.):

2001355051

pISSN:

2146-7277

Keywords:

Abstract:

 Gynostemma pentaphyllum Thunb. Makino is a plant belonging to the Cucurbitaceae family called jiaogulan. The herb of the plant is used for the treatment of diabetes for many years. In the literature, the antidiabetic effect of G. pentaphyllum was determined by in vivo and clinical studies. On the other hand, it was determined that in vitro activity studies aimed at elucidating the mechanism of action of the plant are limited. Although there are commercial preparations containing the plant in America, Europe, China and Asian countries, it was determined that there is no product in Turkiye. Based on these deficiencies, the aim of this study was to determine the antidiabetic activity of G. pentaphyllum, to elucidate its mechanism of action and to perform preliminary phytochemical analyses to prepare standardized extracts in the future. As a result of the studies, it was determined that both methanol and aqueous extracts of the plant showed antidiabetic activity by inhibiting α-amylase and α-glucosidase enzymes under in vitro conditions. The methanol extract was much richer than the aqueous extract in terms of phenolic and flavonoid content. Our research provides preliminary data for the preparation of standardized extracts and food supplements from G. pentaphyllum and is a pioneering work for further studies.

 Gynostemma pentaphyllum Thunb. Makino is a plant belonging to the Cucurbitaceae family called jiaogulan. The herb of the plant is used for the treatment of diabetes for many years. In the literature, the antidiabetic effect of G. pentaphyllum was determined by in vivo and clinical studies. On the other hand, it was determined that in vitro activity studies aimed at elucidating the mechanism of action of the plant are limited. Although there are commercial preparations containing the plant in America, Europe, China and Asian countries, it was determined that there is no product in Turkiye. Based on these deficiencies, the aim of this study was to determine the antidiabetic activity of G. pentaphyllum, to elucidate its mechanism of action and to perform preliminary phytochemical analyses to prepare standardized extracts in the future. As a result of the studies, it was determined that both methanol and aqueous extracts of the plant showed antidiabetic activity by inhibiting α-amylase and α-glucosidase enzymes under in vitro conditions. The methanol extract was much richer than the aqueous extract in terms of phenolic and flavonoid content. Our research provides preliminary data for the preparation of standardized extracts and food supplements from G. pentaphyllum and is a pioneering work for further studies.

 Gynostemma pentaphyllum Thunb. Makino is a plant belonging to the Cucurbitaceae family called jiaogulan. The herb of the plant is used for the treatment of diabetes for many years. In the literature, the antidiabetic effect of G. pentaphyllum was determined by in vivo and clinical studies. On the other hand, it was determined that in vitro activity studies aimed at elucidating the mechanism of action of the plant are limited. Although there are commercial preparations containing the plant in America, Europe, China and Asian countries, it was determined that there is no product in Turkiye. Based on these deficiencies, the aim of this study was to determine the antidiabetic activity of G. pentaphyllum, to elucidate its mechanism of action and to perform preliminary phytochemical analyses to prepare standardized extracts in the future. As a result of the studies, it was determined that both methanol and aqueous extracts of the plant showed antidiabetic activity by inhibiting α-amylase and α-glucosidase enzymes under in vitro conditions. The methanol extract was much richer than the aqueous extract in terms of phenolic and flavonoid content. Our research provides preliminary data for the preparation of standardized extracts and food supplements from G. pentaphyllum and is a pioneering work for further studies.

 Gynostemma pentaphyllum Thunb. Makino, jiaogulan olarak adlandırılan Cucurbitaceae familyasına ait bir bitkidir. Bitkinin toprak üstü kısımları uzun yıllardır diyabet tedavisi için kullanılmaktadır. Literatürde, G. pentaphyllumun antidiyabetik etkisi in vivo ve klinik çalışmalar ile belirlenmiştir. Diğer taraftan bitkinin etki mekanizmasını aydınlatmaya yönelik in vitro aktivite çalışmalarının kısıtlı olduğu tespit edilmiştir. Amerika, Avrupa, Çin ve Asya ülkelerinde bitkiyi içeren ticari preparatları bulunsa da Türkiyede herhangi bir ürünü bulunmadığı belirlenmiştir. Bu eksiklerden yola çıkarak bu çalışmada G. pentaphyllumun antidiyabetik aktivitesinin belirlenmesi, etki mekanizmasının aydınlatılması ve ileride standardize ekstrelerinin hazırlanabilmesi için ön fitokimyasal analizlerinin yapılması amaçlanmıştır. Araştırmalar sonucunda bitkinin hem metanollü hem de sulu ekstresinin in vitro koşullarda α-amilaz ve α-glukozidaz enzimlerini inhibe ederek antidiyabetik aktivite gösterdiği tespit edilmiştir. Fenolik madde ve flavonoit içeriği bakımından metanollü ekstrenin sulu ekstreden çok daha zengin olduğu belirlenmiştir. Araştırmalarımız G. pentaphyllumdan standart ekstre ve gıda takviyesi hazırlanması için ön verileri sağlamakta olup ileri çalışmalar için öncü bir çalışmadır.

Article Details

Preliminary Studies on Gynostemma pentaphyllum (Thunb.) Makino toward Dietary Supplement Development: Antidiabetic Activity and Phytochemical Analysis

Authors:

Methiye Mancak | Fatma Zeynep Uzuner | Ufuk Koca

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Journal:

Karaelmas Fen ve Mühendislik Dergisi Vol.15 Issue 2, pp.15-21

Document Type:

Article

Publication Date:

7/2025

Document Language:

Turkish

Document ID (Org No.):

2001355051

pISSN:

2146-7277

Diabetes

Gynostemma pentaphyllum

HPLC

phytochemical analysis

saponinDiyabet

fitokimyasal analiz

Gynostemma pentaphyllum

saponin

YPSK

Issues

Article Details

Authors: Methiye Mancak | Fatma Zeynep Uzuner | Ufuk Koca

Publisher: Zonguldak Bulent Ecevit University

Main Topics: Sağlık Hizmetleri ve Sistemleri (Diğer)

Journal: Karaelmas Fen ve Mühendislik Dergisi Vol.15 Issue 2, pp.15-21

Document Type: Article

Publication Date: 7/2025

Document Language: Turkish

Document ID (Org No.): 2001355051

pISSN: 2146-7277

Issues

Authors:

Methiye Mancak | Fatma Zeynep Uzuner | Ufuk Koca

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Sağlık Hizmetleri ve Sistemleri (Diğer)

Journal:

Karaelmas Fen ve Mühendislik Dergisi Vol.15 Issue 2, pp.15-21

Document Type:

Article

Publication Date:

7/2025

Document Language:

Turkish

Document ID (Org No.):

2001355051

pISSN:

2146-7277