DNA Barcoding of Liriomyza Sativae Blanchard Agromyzidae, 1938 (Diptera: Agromyzidae

Cem Dayan; Eyüp Mennan Yıldırım

Article Details

DNA Barcoding of Liriomyza Sativae Blanchard Agromyzidae, 1938 (Diptera: Agromyzidae

Authors:

Cem Dayan | Eyüp Mennan Yıldırım

Publisher:

Aydin Adnan Menderes University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.20 Issue 1, pp.167-172

Document Type:

Article

Publication Date:

6/2023

Document Language:

Turkish

Document ID (Org No.):

2001295690

pISSN:

1304-7787

Keywords:

Abstract:

 In this study, DNA barcoding of Liriomyza sativae collected from vegetable areas in Incirliova and Koçarlı districts Aydın was performed between 2020-2022. After the morphological diagnosis of L. sativae, HCOLCO universal primers were used to reproduce the COI gene region of the samples and 612 bp COI regions were obtained. The sequence analysis of the samples was performed by Triogen Biotechnology Istanbul, Türkiye. When the samples were checked through the BLAST located at NCBI, it was found that the species were L. sativae 99-100 and L. trifolii 97-99. The samples were registered to Genbank. As a result of the study, genetic diversity was found to be low.

 In this study, DNA barcoding of Liriomyza sativae collected from vegetable areas in Incirliova and Koçarlı districts Aydın was performed between 2020-2022. After the morphological diagnosis of L. sativae, HCOLCO universal primers were used to reproduce the COI gene region of the samples and 612 bp COI regions were obtained. The sequence analysis of the samples was performed by Triogen Biotechnology Istanbul, Türkiye. When the samples were checked through the BLAST located at NCBI, it was found that the species were L. sativae 99-100 and L. trifolii 97-99. The samples were registered to Genbank. As a result of the study, genetic diversity was found to be low.

 In this study, DNA barcoding of Liriomyza sativae collected from vegetable areas in Incirliova and Koçarlı districts Aydın was performed between 2020-2022. After the morphological diagnosis of L. sativae, HCOLCO universal primers were used to reproduce the COI gene region of the samples and 612 bp COI regions were obtained. The sequence analysis of the samples was performed by Triogen Biotechnology Istanbul, Türkiye. When the samples were checked through the BLAST located at NCBI, it was found that the species were L. sativae 99-100 and L. trifolii 97-99. The samples were registered to Genbank. As a result of the study, genetic diversity was found to be low.

 Bu çalışmada İncirliova ve Koçarlı ilçelerinde Aydın sebze alanlarından toplanmış Liriomyza sativae nin DNA barkodlaması 2020-2022 yılları arasında yapılmıştır. Morfolojik olarak tür teşhisi yapıldıktan sonra; örneklerin COI gen bölgesinin çoğaltılması için HCO LCO evrensel primerler kullanılmış ve 612 bç COI bölgesi elde edilmiştir. Örneklerin sekans analizi Triogen Biyoteknoloji İstanbul-Türkiye tarafından yapılmıştır. Örnekler NCBIda bulunan BLAST aracılığıyla kontrol edildiğinde türlerin L. sativae  99-100 ve L. trifolii 97-99 olduğu görülmüştür. Örnekler Genbanka kaydedilmiştir. Çalışma sonucunda genetik çeşitlilik düşük bulunmuştur.

Article Details

DNA Barcoding of Liriomyza Sativae Blanchard Agromyzidae, 1938 (Diptera: Agromyzidae

Authors:

Cem Dayan | Eyüp Mennan Yıldırım

Publisher:

Aydin Adnan Menderes University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.20 Issue 1, pp.167-172

Document Type:

Article

Publication Date:

6/2023

Document Language:

Turkish

Document ID (Org No.):

2001295690

pISSN:

1304-7787

COI

DNA barcoding

Liriomyza. sativae

identificationCOI

DNA barkodlama

Liriomyza sativae

Tür tanımlama

Issues

Article Details

Authors: Cem Dayan | Eyüp Mennan Yıldırım

Publisher: Aydin Adnan Menderes University

Main Topics: Ziraat Mühendisliği (Diğer)

Journal: Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.20 Issue 1, pp.167-172

Document Type: Article

Publication Date: 6/2023

Document Language: Turkish

Document ID (Org No.): 2001295690

pISSN: 1304-7787

Issues

Authors:

Cem Dayan | Eyüp Mennan Yıldırım

Publisher:

Aydin Adnan Menderes University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Adnan Menderes Üniversitesi Ziraat Fakültesi Dergisi Vol.20 Issue 1, pp.167-172

Document Type:

Article

Publication Date:

6/2023

Document Language:

Turkish

Document ID (Org No.):

2001295690

pISSN:

1304-7787