The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats

İsmetcan İleri; İnci Turan

Article Details

The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats

Authors:

İsmetcan İleri | İnci Turan

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Türkiye Diyabet ve Obezite Dergisi Vol.7 Issue 2, pp.93-101

Document Type:

Article

Publication Date:

8/2023

Document Language:

Turkish

Document ID (Org No.):

2001297825

pISSN:

2587-0335

Keywords:

Abstract:

 Aim: Sleep is one of the crucial factors for muscle metabolism. It has been shown that sleep deprivation SD impairs muscle regeneration. Myostatin is expressed by skeletal muscle cells and limits muscle growth. The purpose of our study was to evaluate the effects of acute sleep deprivation on skeletal muscle myostatin levels in rats. Material and Methods: Twenty one male Wistar albino rats 200-250g were randomly allocated in to three groups n7: Control group, Wide platform WP group and SD group. REM SD was induced by using the modified multiple platform method for 72 hours. The locomotor activity were evaluated among groups using open field test OFT. Gastrocnemius and soleus muscle tissue were harvested and the levels of myostatin, malondialdehyde MDA, glutathione GSH and glycogen was measured in the muscle tissues. Results: This study showed that acute SD caused an increase in myostatin 116139.55 and MDA levels 115.378.47 in the soleus muscle compared to the control group respectively p0.036 ve p0.01. Compared with the other groups, the number of crossing square was increased in OFT 56.3322.02 p0.001 ve p0.044. GSH 4.860.26 and glycogen levels 5.130.21 in gastrocnemius muscle was decresead in SD group respectively p0.007 ve p0.028. Conclusion: These data may represent that REM sleep deprivation affects muscle metabolism by changing myostatin levels and oxidative stress parameters in skeletal muscle to different extents in different muscle types.

 Aim: Sleep is one of the crucial factors for muscle metabolism. It has been shown that sleep deprivation SD impairs muscle regeneration. Myostatin is expressed by skeletal muscle cells and limits muscle growth. The purpose of our study was to evaluate the effects of acute sleep deprivation on skeletal muscle myostatin levels in rats. Material and Methods: Twenty one male Wistar albino rats 200-250g were randomly allocated in to three groups n7: Control group, Wide platform WP group and SD group. REM SD was induced by using the modified multiple platform method for 72 hours. The locomotor activity were evaluated among groups using open field test OFT. Gastrocnemius and soleus muscle tissue were harvested and the levels of myostatin, malondialdehyde MDA, glutathione GSH and glycogen was measured in the muscle tissues. Results: This study showed that acute SD caused an increase in myostatin 116139.55 and MDA levels 115.378.47 in the soleus muscle compared to the control group respectively p0.036 ve p0.01. Compared with the other groups, the number of crossing square was increased in OFT 56.3322.02 p0.001 ve p0.044. GSH 4.860.26 and glycogen levels 5.130.21 in gastrocnemius muscle was decresead in SD group respectively p0.007 ve p0.028. Conclusion: These data may represent that REM sleep deprivation affects muscle metabolism by changing myostatin levels and oxidative stress parameters in skeletal muscle to different extents in different muscle types.

 Aim: Sleep is one of the crucial factors for muscle metabolism. It has been shown that sleep deprivation SD impairs muscle regeneration. Myostatin is expressed by skeletal muscle cells and limits muscle growth. The purpose of our study was to evaluate the effects of acute sleep deprivation on skeletal muscle myostatin levels in rats. Material and Methods: Twenty one male Wistar albino rats 200-250g were randomly allocated in to three groups n7: Control group, Wide platform WP group and SD group. REM SD was induced by using the modified multiple platform method for 72 hours. The locomotor activity were evaluated among groups using open field test OFT. Gastrocnemius and soleus muscle tissue were harvested and the levels of myostatin, malondialdehyde MDA, glutathione GSH and glycogen was measured in the muscle tissues. Results: This study showed that acute SD caused an increase in myostatin 116139.55 and MDA levels 115.378.47 in the soleus muscle compared to the control group respectively p0.036 ve p0.01. Compared with the other groups, the number of crossing square was increased in OFT 56.3322.02 p0.001 ve p0.044. GSH 4.860.26 and glycogen levels 5.130.21 in gastrocnemius muscle was decresead in SD group respectively p0.007 ve p0.028. Conclusion: These data may represent that REM sleep deprivation affects muscle metabolism by changing myostatin levels and oxidative stress parameters in skeletal muscle to different extents in different muscle types.

 Amaç: Uyku, kas metabolizması için önemli faktörlerden biridir. Uyku yoksunluğunun UY kas rejenerasyonunu bozduğu gösterilmiştir. Myostatin iskelet kası hücreleri tarafından eksprese edilir ve kas büyümesini sınırlandırır. Çalışmamızın amacı, sıçanlarda akut uyku yoksunluğunun iskelet kası myostatin düzeyleri üzerindeki etkilerini değerlendirmektir. Gereç ve Yöntemler: Yirmi bir erkek Wistar albino sıçan 200-250g rastgele üç gruba n7 ayrılmıştır: Kontrol grubu, Geniş platform GP grubu ve UY grubu. REM UY, 72 saat boyunca modifiye çoklu platform yöntemi kullanılarak indüklenmiştir. Lökomotor aktivite gruplar arasında açık alan testi OFT kullanılarak değerlendirilmiştir. Gastroknemius ve soleus kas dokuları alınmış ve kas dokularında myostatin, malondialdehit MDA, glutatyon GSH ve glikojen seviyeleri ölçülmüştür. Bulgular: Bu çalışma, akut UYnin kontrol grubuna kıyasla soleus kasında myostatin 116139,55 ve MDA 115,378,47 seviyelerinde artışa neden olduğunu göstermiştir sırasıyla p0,036 ve p0,01. OFTde geçilen kare sayısı diğer gruplarla karşılaştırıldığında artmıştır 56,3322,02 p0,001 ve p0,044. Gastroknemius kasında GSH 4,860,26 ve glikojen seviyeleri 5,130,21 UY grubunda azalmıştır sırasıyla p0,007 ve p0,028. Sonuç: Bu veriler, REM uyku yoksunluğunun iskelet kasındaki myostatin seviyelerini ve oksidatif stres parametrelerini farklı kas tiplerinde farklı miktarlarda değiştirerek kas metabolizmasını etkilediğini gösterebilir.

Article Details

The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats

Authors:

İsmetcan İleri | İnci Turan

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Türkiye Diyabet ve Obezite Dergisi Vol.7 Issue 2, pp.93-101

Document Type:

Article

Publication Date:

8/2023

Document Language:

Turkish

Document ID (Org No.):

2001297825

pISSN:

2587-0335

acute REM sleep deprivation

myostatin

skeletal muscleAkut REM uyku yoksunluğu

miyostatin

iskelet kası

Issues

Article Details

The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats

Authors: İsmetcan İleri | İnci Turan

Publisher: Zonguldak Bulent Ecevit University

Main Topics: Tıbbi Fizyoloji (Diğer)

Journal: Türkiye Diyabet ve Obezite Dergisi Vol.7 Issue 2, pp.93-101

Document Type: Article

Publication Date: 8/2023

Document Language: Turkish

Document ID (Org No.): 2001297825

pISSN: 2587-0335

acute REM sleep deprivation

myostatin

skeletal muscleAkut REM uyku yoksunluğu

miyostatin

iskelet kası

Issues

Authors:

İsmetcan İleri | İnci Turan

Publisher:

Zonguldak Bulent Ecevit University

Main Topics:

Tıbbi Fizyoloji (Diğer)

Journal:

Türkiye Diyabet ve Obezite Dergisi Vol.7 Issue 2, pp.93-101

Document Type:

Article

Publication Date:

8/2023

Document Language:

Turkish

Document ID (Org No.):

2001297825

pISSN:

2587-0335