Purification and Characterization of α-Carbonic Anhydrase II from Sheep Liver and Examining the Inhibition Effect of Kanamycin on Enzyme Activity

Veysel ÇOMAKLI; Emrah YERLİKAYA; Ramazan DEMİRDAĞ; Ömer İrfan KÜFREVİOĞLU

Article Details

Purification and Characterization of α-Carbonic Anhydrase II from Sheep Liver and Examining the Inhibition Effect of Kanamycin on Enzyme Activity

Authors:

Veysel ÇOMAKLI | Emrah YERLİKAYA | Ramazan DEMİRDAĞ | Ömer İrfan KÜFREVİOĞLU

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2012 Vol.40 Issue 2, pp.133-138

Document Type:

Article

Publication Date:

3/2012

Document Language:

English

Document ID (Org No.):

2000449480

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X

Abstract:

 

Sheep carbonic anhydrase - II  SCA-II   E.C: 4.2.1.1  was purified from sheep liver and some characteristic properties were investigated. The enzyme was purified approximate 43.1-fold with a yield of 38.6%, and a specific activity of 4000 EU/mg proteins. For the enzyme, optimum pH, optimum temperature, optimum ionic strength and stable pH were determined to be 7.5, 40ºC, 10 mM and 8.5, respectively. The molecular weight was found 29 kDa by sodium dodecyl sulfate polyacrylamide gel electrophoresis  SDS-PAGE . Kanamycin ex- hibited in vitro inhibitory effect on the enzyme activity.

 

K oyun karbonik anhidraz-II  E.C: 4.2.1.1  enzimi koyun karaciğerinden saflaştırıldı ve bazı karakteristik özellikleri araştırıldı. Enzim 4000 EU/mg protein spesifik aktivitesi ve % 38.6 verim ile yaklaşık olarak 43,1 kat saflaştırıldı. Enzim için optimum pH, optimum sıcaklık, optimum iyonik şiddet ve stabil pH sırasıyla 7.5, 40ºC, 10 mM ve 8.5 olarak belirlendi. Moleküler ağırlığı sodyum dodesil sülfat poliakrilamid jel elektroforezi ile 29 kDa olarak bulundu. Kanamisinin enzim aktivitesi üzerine in vitro inhibitör etkisi incelendi.

Article Details

Purification and Characterization of α-Carbonic Anhydrase II from Sheep Liver and Examining the Inhibition Effect of Kanamycin on Enzyme Activity

Authors: Veysel ÇOMAKLI | Emrah YERLİKAYA | Ramazan DEMİRDAĞ | Ömer İrfan KÜFREVİOĞLU

Publisher: Hacettepe University

Journal: Hacettepe Journal of Biology and Chemistry 2012 Vol.40 Issue 2, pp.133-138

Document Type: Article

Publication Date: 3/2012

Document Language: English

Document ID (Org No.): 2000449480

Frequency: Quarterly

eISSN: 2687-475X

pISSN: 2687-475X

Issues

Authors:

Veysel ÇOMAKLI | Emrah YERLİKAYA | Ramazan DEMİRDAĞ | Ömer İrfan KÜFREVİOĞLU

Publisher:

Hacettepe University

Journal:

Hacettepe Journal of Biology and Chemistry 2012 Vol.40 Issue 2, pp.133-138

Document Type:

Article

Publication Date:

3/2012

Document Language:

English

Document ID (Org No.):

2000449480

Frequency:

Quarterly

eISSN:

2687-475X

pISSN:

2687-475X