In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds

Aris TERCAN; Özlem SAÇAN

Article Details

In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds

Authors:

Aris TERCAN | Özlem SAÇAN

Publisher:

Istanbul University

Main Topics:

Pharmacology and therapeutics | Promotion of health

Journal:

İstanbul Journal of Pharmacy 2020 Vol.50 Issue 3, pp.283-288

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2000512941

Frequency:

Monthly

eISSN:

2587-2087

Abstract:

 

Background and Aims: Adenosine deaminase (EC 3.5.4.4, ADA) is distributed in all human tissues. It catalyses the deamination of adenosine (deoxyadenosine) to inosine (deoxyinosine) via removal of an amino group. The determination of ADAinhibition is of extreme significance in the field of fundamental research and clinical diagnosis. In our study, the effects of various plant extracts and chemicals on ADA activity were investigated. Methods: Adenosine deaminase activity was determined spectrophotometrically at 625 nm. Results: The inhibitory activities of the extracts and chemical compounds increased in a dose-dependent manner. Among the plant extracts used, lemon extract was observed to exhibit the highest ADA inhibition activity with an IC50 value of 0.05 ± 0.001 mg/mL, while quercetin had the highest ADA inhibition (IC50 value of 0.004±0.0005 mg/mL) among the chemical compounds. All plant extracts and chemical compounds showed ADA inhibition activities. Conclusion: The obtained results indicate that plant extracts and some chemical compounds examined in this study can be a potential source of novel ADA-inhibitors for therapeutics.

Article Details

In vitro adenosine deaminase inhibitory activity of some selected plant extracts and chemical compounds

Authors: Aris TERCAN | Özlem SAÇAN

Publisher: Istanbul University

Main Topics: Pharmacology and therapeutics | Promotion of health

Journal: İstanbul Journal of Pharmacy 2020 Vol.50 Issue 3, pp.283-288

Document Type: Article

Publication Date: 12/2020

Document Language: English

Document ID (Org No.): 2000512941

Frequency: Monthly

eISSN: 2587-2087

Issues

Authors:

Aris TERCAN | Özlem SAÇAN

Publisher:

Istanbul University

Main Topics:

Pharmacology and therapeutics | Promotion of health

Journal:

İstanbul Journal of Pharmacy 2020 Vol.50 Issue 3, pp.283-288

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2000512941

Frequency:

Monthly

eISSN:

2587-2087