IN VITRO EVALUATION OF THE ANTIBACTERIAL EFFECT OF CHITOSAN COATED TEICOPLANIN-LOADED LIPID NANOPARTICLES AGAINST STAPHYLOCOCCUS AUREUS

Berrin Küçüktürkmen; Merve Eylül Kıymacı

Article Details

IN VITRO EVALUATION OF THE ANTIBACTERIAL EFFECT OF CHITOSAN COATED TEICOPLANIN-LOADED LIPID NANOPARTICLES AGAINST STAPHYLOCOCCUS AUREUS

Authors:

Berrin Küçüktürkmen | Merve Eylül Kıymacı

Publisher:

Ankara University

Main Topics:

İlaç Dağıtım Teknolojileri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1050-1057

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001319087

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: The aim of this research is to formulate teicoplanin-loaded solid lipid nanoparticles SLN coated with chitosan to sustain teicoplanin release for effective antibacterial therapy. Material and Method: Double emulsion-solvent evaporation technique was used for the production of SLNs. The nanoparticles were characterised in terms of morphology, size, encapsulation efficacy, in-vitro drug release and antibacterial activity studies after optimization of process and formulation parameters. Result and Discussion: Transmission electron microscopy images confirmed the formation of spherical SLNs. With chitosan coating, the size increased from 80 nm to 106 nm and the negative value of zeta potential - 11.29 changed to positive 34.41. The in-vitro release data showed prolonged release of teicoplanin from optimized SLN and chitosan-coated SLN c-SLN formulations over 1 week. The antibacterial activity study S. aureus and Methicillin-resistant S. aureus showed the activity of the teicoplanin loaded SLN formulations. In conclusion, this study demonstrated the potential of c-SLN for effective delivery of teicoplanin.

 Objective: The aim of this research is to formulate teicoplanin-loaded solid lipid nanoparticles SLN coated with chitosan to sustain teicoplanin release for effective antibacterial therapy. Material and Method: Double emulsion-solvent evaporation technique was used for the production of SLNs. The nanoparticles were characterised in terms of morphology, size, encapsulation efficacy, in-vitro drug release and antibacterial activity studies after optimization of process and formulation parameters. Result and Discussion: Transmission electron microscopy images confirmed the formation of spherical SLNs. With chitosan coating, the size increased from 80 nm to 106 nm and the negative value of zeta potential - 11.29 changed to positive 34.41. The in-vitro release data showed prolonged release of teicoplanin from optimized SLN and chitosan-coated SLN c-SLN formulations over 1 week. The antibacterial activity study S. aureus and Methicillin-resistant S. aureus showed the activity of the teicoplanin loaded SLN formulations. In conclusion, this study demonstrated the potential of c-SLN for effective delivery of teicoplanin.

 Objective: The aim of this research is to formulate teicoplanin-loaded solid lipid nanoparticles SLN coated with chitosan to sustain teicoplanin release for effective antibacterial therapy. Material and Method: Double emulsion-solvent evaporation technique was used for the production of SLNs. The nanoparticles were characterised in terms of morphology, size, encapsulation efficacy, in-vitro drug release and antibacterial activity studies after optimization of process and formulation parameters. Result and Discussion: Transmission electron microscopy images confirmed the formation of spherical SLNs. With chitosan coating, the size increased from 80 nm to 106 nm and the negative value of zeta potential - 11.29 changed to positive 34.41. The in-vitro release data showed prolonged release of teicoplanin from optimized SLN and chitosan-coated SLN c-SLN formulations over 1 week. The antibacterial activity study S. aureus and Methicillin-resistant S. aureus showed the activity of the teicoplanin loaded SLN formulations. In conclusion, this study demonstrated the potential of c-SLN for effective delivery of teicoplanin.

 Amaç: Bu araştırmanın amacı, etkili antibakteriyel tedavi için teikoplanin salımını uzatmak üzere kitosanla kaplanmış teikoplanin yüklü katı lipit nanopartiküllerinin SLN formüle edilmesidir. Gereç ve Yöntem: SLNler çift emülsifikasyon-solvent buharlaştırma tekniği ile hazırlanmıştır. Proses ve formülasyon parametrelerinin optimizasyonu sonrasında nanopartiküller morfoloji, boyut, enkapsülasyon etkinliği, in vitro etken madde salımı ve antibakteriyel aktivite çalışmaları açısından karakterize edilmiştir. Sonuç ve Tartışma: Geçirimli elektron mikroskobu görüntüleri küresel SLNlerin oluşumunu doğrulamıştır. Kitosan kaplama ile boyut artmış 80 nmden 106 nmye ve zeta potansiyelinin negatif değeri -11.29 pozitife 34.41 dönüşmüştür. İn vitro salım verileri, optimize edilmiş SLN ve kitosan kaplı SLN c-SLN formülasyonlarından teikoplaninin 1 hafta boyunca uzun süreli salınımını göstermiştir. Antibakteriyel aktivite çalışması S. aureus ve Metisiline dirençli S. aureus, teikoplanin yüklü SLN formülasyonlarının aktivitesini göstermiştir. Sonuç olarak, bu çalışma c-SLNnin teikoplaninin etkin verilişi için potansiyelini ortaya koymuştur.

Article Details

IN VITRO EVALUATION OF THE ANTIBACTERIAL EFFECT OF CHITOSAN COATED TEICOPLANIN-LOADED LIPID NANOPARTICLES AGAINST STAPHYLOCOCCUS AUREUS

Authors:

Berrin Küçüktürkmen | Merve Eylül Kıymacı

Publisher:

Ankara University

Main Topics:

İlaç Dağıtım Teknolojileri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1050-1057

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001319087

pISSN:

1015-3918

Chitosan

coating

lipid nanoparticle

precirol ATO 5

teicoplaninKaplama

kitosan

lipit nanopartikül

precirol ATO 5

teikoplanin

Issues

Article Details

Authors: Berrin Küçüktürkmen | Merve Eylül Kıymacı

Publisher: Ankara University

Main Topics: İlaç Dağıtım Teknolojileri

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1050-1057

Document Type: Article

Publication Date: 10/2024

Document Language: English

Document ID (Org No.): 2001319087

pISSN: 1015-3918

Issues

Authors:

Berrin Küçüktürkmen | Merve Eylül Kıymacı

Publisher:

Ankara University

Main Topics:

İlaç Dağıtım Teknolojileri

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 3, pp.1050-1057

Document Type:

Article

Publication Date:

10/2024

Document Language:

English

Document ID (Org No.):

2001319087

pISSN:

1015-3918