Determination of the DNA and Amino Acid Sequences of the Lactate Dehydrogenase Gene from Plasmodium falciparum Strains K1 and PF FCBR: A Route to the Design of  New Antimalarials

Dilek TURGUT BALIK

Article Details

Determination of the DNA and Amino Acid Sequences of the Lactate Dehydrogenase Gene from Plasmodium falciparum Strains K1 and PF FCBR: A Route to the Design of New Antimalarials

Authors:

Dilek TURGUT BALIK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2001 Vol.25 Issue 3, pp.241-250

Document Type:

Article

Publication Date:

2/2001

Document Language:

Turkish

Document ID (Org No.):

2000813809

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

This paper describes cloning of the gene coding for the lactate dehydrogenase (LDH) gene from the two different strains of Plasmodium falciparum, K1 and PF FCBR. The DNA sequences of LDH genes of these two strains were found to be identical. Amino acid sequence alignment of LDH from P. falciparum strains K1 and PF FCBR (PfLDH) to some other known LDH sequences showed that PfLDH has 29% residue identities with Bacillus stearothermophilus (BsLDH) and 29%, 31%, 33% with dogfish, man-M4, and pig-M4 LDHs, respectively. It was also shown that PfLDH has insertions and single amino acid deletions. Two deletions are glutamate-48 and glycine-217. It has a single amino acid insertion, a tyrosine, between residues 73 and 74, and most remarkably a five residue insertion in the catalytic loop compared to other LDH sequences. This five residue insertion could be exploited in drug design.

 

This paper describes cloning of the gene coding for the lactate dehydrogenase (LDH) gene from the two different strains of Plasmodium falciparum, K1 and PF FCBR. The DNA sequences of LDH genes of these two strains were found to be identical. Amino acid sequence alignment of LDH from P. falciparum strains K1 and PF FCBR (PfLDH) to some other known LDH sequences showed that PfLDH has 29% residue identities with Bacillus stearothermophilus (BsLDH) and 29%, 31%, 33% with dogfish, man-M4, and pig-M4 LDHs, respectively. It was also shown that PfLDH has insertions and single amino acid deletions. Two deletions are glutamate-48 and glycine-217. It has a single amino acid insertion, a tyrosine, between residues 73 and 74, and most remarkably a five residue insertion in the catalytic loop compared to other LDH sequences. This five residue insertion could be exploited in drug design.

Article Details

Determination of the DNA and Amino Acid Sequences of the Lactate Dehydrogenase Gene from Plasmodium falciparum Strains K1 and PF FCBR: A Route to the Design of New Antimalarials

Authors:

Dilek TURGUT BALIK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2001 Vol.25 Issue 3, pp.241-250

Document Type:

Article

Publication Date:

2/2001

Document Language:

Turkish

Document ID (Org No.):

2000813809

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

lactate dehydrogenase

antimalarials

DNA sequencing

gene cloning

catalytic loop

Issues

Article Details

Determination of the DNA and Amino Acid Sequences of the Lactate Dehydrogenase Gene from Plasmodium falciparum Strains K1 and PF FCBR: A Route to the Design of New Antimalarials

Authors: Dilek TURGUT BALIK

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2001 Vol.25 Issue 3, pp.241-250

Document Type: Article

Publication Date: 2/2001

Document Language: Turkish

Document ID (Org No.): 2000813809

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

lactate dehydrogenase

antimalarials

DNA sequencing

gene cloning

catalytic loop

Issues

Authors:

Dilek TURGUT BALIK

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2001 Vol.25 Issue 3, pp.241-250

Document Type:

Article

Publication Date:

2/2001

Document Language:

Turkish

Document ID (Org No.):

2000813809

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152