Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Sandeep Kumar VERMA; Bahtiyar Buhara YÜCESAN; Günce ŞAHİN

Article Details

Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Authors:

Sandeep Kumar VERMA | Bahtiyar Buhara YÜCESAN | Günce ŞAHİN

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2011 Vol.35 Issue 6, pp.689-695

Document Type:

Article

Publication Date:

5/2011

Document Language:

Turkish

Document ID (Org No.):

2000723098

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X

Abstract:

 

This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. (dwarf foxglove) via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA) with BAP, kinetin, TDZ, and zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the first set of experiments. For shoot regeneration (the first set of experiments), TDZ was the most effective at 1.0 mg L-1 concentration, producing a mean of 10.3 shoots per explant and was significantly more effective than BAP. For shoot multiplication (the second set of experiments), a combination of 0.2 mg L-1 IBA with 0.2 mg L-1 TDZ produced significantly more shoots per explant (16.5 shoots) than with BAP (11.0 shoots), zeatin (5.5 shoots), or kinetin (4.0 shoots). The regenerated shoots were readily rooted on medium containing 0.5 mg L-1 indole-3-acetic acid (IAA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. The described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of cardenolides, germplasm conservation, and genetic transformation studies in this medicinally important endemic species.

 

This study reports, for the first time, an efficient in vitro plant regeneration protocol for Digitalis lamarckii Ivan. (dwarf foxglove) via direct shoot organogenesis. Two sets of experiments were carried out; the first compared different concentrations of 6-benzylaminopurine (BAP), kinetin, thidiazuron (TDZ), and zeatin alone using leaf explants excised from in vitro germinated seedlings, while the second set tested the combinations of indole-3-butyric acid (IBA) with BAP, kinetin, TDZ, and zeatin for shoot multiplication from the leaf explants, which were already cultured and developed numerous shoots during the first set of experiments. For shoot regeneration (the first set of experiments), TDZ was the most effective at 1.0 mg L-1 concentration, producing a mean of 10.3 shoots per explant and was significantly more effective than BAP. For shoot multiplication (the second set of experiments), a combination of 0.2 mg L-1 IBA with 0.2 mg L-1 TDZ produced significantly more shoots per explant (16.5 shoots) than with BAP (11.0 shoots), zeatin (5.5 shoots), or kinetin (4.0 shoots). The regenerated shoots were readily rooted on medium containing 0.5 mg L-1 indole-3-acetic acid (IAA). Rooted regenerants were then transferred to the pots, where they grew well and attained maturity. The described protocol provides a simple way to regenerate plants through direct shoot organogenesis, which would be useful for a large-scale production of cardenolides, germplasm conservation, and genetic transformation studies in this medicinally important endemic species.

Article Details

Direct shoot regeneration from leaf explants of Digitalis lamarckii, an endemic medicinal species

Authors: Sandeep Kumar VERMA | Bahtiyar Buhara YÜCESAN | Günce ŞAHİN

Publisher: TUBITAK

Journal: Turkish Journal of Botany 2011 Vol.35 Issue 6, pp.689-695

Document Type: Article

Publication Date: 5/2011

Document Language: Turkish

Document ID (Org No.): 2000723098

Frequency: Bimonthly

eISSN: 1303-6106

pISSN: 1300-008X

Issues

Authors:

Sandeep Kumar VERMA | Bahtiyar Buhara YÜCESAN | Günce ŞAHİN

Publisher:

TUBITAK

Journal:

Turkish Journal of Botany 2011 Vol.35 Issue 6, pp.689-695

Document Type:

Article

Publication Date:

5/2011

Document Language:

Turkish

Document ID (Org No.):

2000723098

Frequency:

Bimonthly

eISSN:

1303-6106

pISSN:

1300-008X