CLONING AND NUCLEOTİDE SEQUENCE ANALYSIS OF A B. subtilis DNA FRAGMENT CARRYING CAR GENE CODING FOR CARBAMOYL PHOSPHATE SYNTHETASE ENZYME

Ahmet KADIKIRAN

doi:https://doi.org/10.1501/Commuc_0000000136

Article Details

CLONING AND NUCLEOTİDE SEQUENCE ANALYSIS OF A B. subtilis DNA FRAGMENT CARRYING CAR GENE CODING FOR CARBAMOYL PHOSPHATE SYNTHETASE ENZYME

Authors:

Ahmet KADIKIRAN

Publisher:

Ankara University

Main Topics:

Life sciences

Journal:

Communications Faculty of Sciences University of Ankara Series C Biology 1988 Vol.06 (1988)

Document Type:

Article

Publication Date:

12/1987

Document Language:

English

Document ID (Org No.):

2000911421

Frequency:

Monthly

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/Commuc_0000000136

Abstract:

 

A B. subtvis DNA fragment (1229 bp) known to carry ar gene coding for carbamoyl phosphate synthetase enzyme was cloned in pBB322 and the restriction analysis of this fragment was carried ou? (Kadıkıran, 1988a).
The 1229 bp fragment was then cloned in M13mp8 and Mİ 3 mp9 seguencing veeters.
Seguencing was carried out by the dideoyy chain termination method. Subcloning was emp- loyed to insure the fidelity of seguencing.
Of the 1229 bp, 796 bp have been documented while 433 bp streteh of DNA corresponding tc the ieft oi centre on the fragment has not been inciuded due to the further clarifications nee- ded.
Restriction site positions on vhe fragment for the restriction enzymes used for restriction analy sis have been refined further.
Probable premoter consensuı sequences for -35 promoter up-stream, -10 Pribnow-box, -t-1 start signal sequences have been proposed.

Article Details

CLONING AND NUCLEOTİDE SEQUENCE ANALYSIS OF A B. subtilis DNA FRAGMENT CARRYING CAR GENE CODING FOR CARBAMOYL PHOSPHATE SYNTHETASE ENZYME

Authors: Ahmet KADIKIRAN

Publisher: Ankara University

Main Topics: Life sciences

Journal: Communications Faculty of Sciences University of Ankara Series C Biology 1988 Vol.06 (1988)

Document Type: Article

Publication Date: 12/1987

Document Language: English

Document ID (Org No.): 2000911421

Frequency: Monthly

eISSN: 2651-3749

pISSN: 1303-6025

DOI: https://doi.org/10.1501/Commuc_0000000136

Issues

Authors:

Ahmet KADIKIRAN

Publisher:

Ankara University

Main Topics:

Life sciences

Journal:

Communications Faculty of Sciences University of Ankara Series C Biology 1988 Vol.06 (1988)

Document Type:

Article

Publication Date:

12/1987

Document Language:

English

Document ID (Org No.):

2000911421

Frequency:

Monthly

eISSN:

2651-3749

pISSN:

1303-6025

DOI:

https://doi.org/10.1501/Commuc_0000000136