CARBON NANOTUBES MODIFIED ELISA SYSTEM DEVELOPMENT FOR IMPROVED DETECTION OF BIOMARKERS

Nimet Yıldırım Tirgil

Article Details

CARBON NANOTUBES MODIFIED ELISA SYSTEM DEVELOPMENT FOR IMPROVED DETECTION OF BIOMARKERS

Authors:

Nimet Yıldırım Tirgil

Publisher:

Mugla Sitki Kocman University

Main Topics:

Mühendislik

Journal:

Mugla Journal of Science and Technology Vol.9 Issue 1, pp.80-86

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001292886

pISSN:

2149-3596

Keywords:

Abstract:

 This study developed and evaluated a nanomaterial-modified ELISA system to analyze a peptide biomarker Human VEGF. Multiwalled carbon nanotubes MWCNTs were used as nanomaterial-based modifiers for the capturedetection antibodies and HRP enzyme. CNTs modifications of the ELISA method at these three different binding steps were tested and compared. The most efficient modification step was concluded depending on these works sensitivities, linearities, and repeatabilities. We proved that CNTs could be used successfully to enhance the performances compared to the traditional ELISA method by achieving higher sensitivities and, therefore, more confident results, especially with a 0.5 pgml of limit of detection value. In addition, the performances of different modification steps in the commercial serum sample and the stability of all these binding steps were evaluated, and compared with the traditional ELISA method.

 This study developed and evaluated a nanomaterial-modified ELISA system to analyze a peptide biomarker Human VEGF. Multiwalled carbon nanotubes MWCNTs were used as nanomaterial-based modifiers for the capturedetection antibodies and HRP enzyme. CNTs modifications of the ELISA method at these three different binding steps were tested and compared. The most efficient modification step was concluded depending on these works sensitivities, linearities, and repeatabilities. We proved that CNTs could be used successfully to enhance the performances compared to the traditional ELISA method by achieving higher sensitivities and, therefore, more confident results, especially with a 0.5 pgml of limit of detection value. In addition, the performances of different modification steps in the commercial serum sample and the stability of all these binding steps were evaluated, and compared with the traditional ELISA method.

 This study developed and evaluated a nanomaterial-modified ELISA system to analyze a peptide biomarker Human VEGF. Multiwalled carbon nanotubes MWCNTs were used as nanomaterial-based modifiers for the capturedetection antibodies and HRP enzyme. CNTs modifications of the ELISA method at these three different binding steps were tested and compared. The most efficient modification step was concluded depending on these works sensitivities, linearities, and repeatabilities. We proved that CNTs could be used successfully to enhance the performances compared to the traditional ELISA method by achieving higher sensitivities and, therefore, more confident results, especially with a 0.5 pgml of limit of detection value. In addition, the performances of different modification steps in the commercial serum sample and the stability of all these binding steps were evaluated, and compared with the traditional ELISA method.

 Bu çalışmada, bir peptit biyobelirtecini İnsan VEGF analiz etmek için nanomateryalle modifiye edilmiş bir ELISA sistemi geliştirdi ve değerlendirdi. Çok duvarlı karbon nanotüpler MWCNT, yakalamatespit antikorları ve HRP enzimi için nanomateryal bazlı modifiye malzemesi olarak kullanıldı. ELISA yönteminin bu üç farklı bağlanma aşamasındaki CNT modifikasyonları test edildi ve karşılaştırıldı. Bu çalışmaların hassasiyet, doğrusallık ve tekrarlanabilirliklerine bağlı olarak en verimli modifikasyon adımı belirlendi. CNTlerin kullanıldığı ELISA metodunda, özellikle 0,5 pgml tespit limit değeri ile daha yüksek hassasiyetler ve dolayısıyla daha güvenilir sonuçlar elde edildiği ve geleneksel ELISA yönteminede performansı artırmak için başarılı bir şekilde kullanılabileceğini kanıtlandı. Ayrıca ticari serum örneklerinin analizinde farklı modifikasyon adımlarının performansları ve tüm bu bağlanma adımlarının stabilitesi değerlendirildi ve geleneksel ELISA yöntemi ile karşılaştırıldı.

Article Details

CARBON NANOTUBES MODIFIED ELISA SYSTEM DEVELOPMENT FOR IMPROVED DETECTION OF BIOMARKERS

Authors:

Nimet Yıldırım Tirgil

Publisher:

Mugla Sitki Kocman University

Main Topics:

Mühendislik

Journal:

Mugla Journal of Science and Technology Vol.9 Issue 1, pp.80-86

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001292886

pISSN:

2149-3596

ELISA

Carbon nanotube

Bioanalysis

BiotechnologyELISA

Karbon nanotüp

Biyoanaliz

Biyoteknoloji

Issues

Article Details

Authors: Nimet Yıldırım Tirgil

Publisher: Mugla Sitki Kocman University

Main Topics: Mühendislik

Journal: Mugla Journal of Science and Technology Vol.9 Issue 1, pp.80-86

Document Type: Article

Publication Date: 6/2023

Document Language: English

Document ID (Org No.): 2001292886

pISSN: 2149-3596

Issues

Authors:

Nimet Yıldırım Tirgil

Publisher:

Mugla Sitki Kocman University

Main Topics:

Mühendislik

Journal:

Mugla Journal of Science and Technology Vol.9 Issue 1, pp.80-86

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001292886

pISSN:

2149-3596