Biological activities and luteolin derivatives of Verbascum  eskisehirensis Karavel., Ocak & Ekici

Gözde Öztürk; Hale Gamze Ağalar; Fatih Göğer

Article Details

Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak & Ekici

Authors:

Gözde Öztürk | Hale Gamze Ağalar | Fatih Göğer

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.532-542

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347200

pISSN:

2630-6344

Keywords:

Abstract:

 In this present study, the aerial parts of Verbascum eskisehirensis were dried in the shade and air-flowed area. The dried samples were macerated with 70 methanol in water for 24 h by three times. The biological activities of the extract was determined by in vitro methods. The antioxidant activity was evaluated in 2,2-diphenyl-1 picrylhydrazyl DPPH radical scavenging assay and the trolox equivalent antioxidant capacity TEAC was measured the ability of the extract to scavenge the stable radical cation ABTS 2,2-azinobis3-ethylbenzothiazoline-6-sulfonic acid. The antibacterial effects against some clinical pathogens were tested by broth microdilution assay. The in vitro antiinflammatory activity was also examined by 5-Lipoxygenase 5-LOX enzyme inhibitory test. In the same time, the phenolic composition of the extract was determined by LC-ESI-MSMS system. According to results, the extract was found rich-in luteolin derivatives. The major compound was identified as luteolin-pentosyl-hexoside. The antioxidant activities of the extract were moderate. The extract showed weak antibacterial activity against tested bacteria strains. Salmonella typhimurium ATCC 13311 was more sensitive to the extract than the others. The extract showed weak antiinflammatory activity.

 In this present study, the aerial parts of Verbascum eskisehirensis were dried in the shade and air-flowed area. The dried samples were macerated with 70 methanol in water for 24 h by three times. The biological activities of the extract was determined by in vitro methods. The antioxidant activity was evaluated in 2,2-diphenyl-1 picrylhydrazyl DPPH radical scavenging assay and the trolox equivalent antioxidant capacity TEAC was measured the ability of the extract to scavenge the stable radical cation ABTS 2,2-azinobis3-ethylbenzothiazoline-6-sulfonic acid. The antibacterial effects against some clinical pathogens were tested by broth microdilution assay. The in vitro antiinflammatory activity was also examined by 5-Lipoxygenase 5-LOX enzyme inhibitory test. In the same time, the phenolic composition of the extract was determined by LC-ESI-MSMS system. According to results, the extract was found rich-in luteolin derivatives. The major compound was identified as luteolin-pentosyl-hexoside. The antioxidant activities of the extract were moderate. The extract showed weak antibacterial activity against tested bacteria strains. Salmonella typhimurium ATCC 13311 was more sensitive to the extract than the others. The extract showed weak antiinflammatory activity.

 In this present study, the aerial parts of Verbascum eskisehirensis were dried in the shade and air-flowed area. The dried samples were macerated with 70 methanol in water for 24 h by three times. The biological activities of the extract was determined by in vitro methods. The antioxidant activity was evaluated in 2,2-diphenyl-1 picrylhydrazyl DPPH radical scavenging assay and the trolox equivalent antioxidant capacity TEAC was measured the ability of the extract to scavenge the stable radical cation ABTS 2,2-azinobis3-ethylbenzothiazoline-6-sulfonic acid. The antibacterial effects against some clinical pathogens were tested by broth microdilution assay. The in vitro antiinflammatory activity was also examined by 5-Lipoxygenase 5-LOX enzyme inhibitory test. In the same time, the phenolic composition of the extract was determined by LC-ESI-MSMS system. According to results, the extract was found rich-in luteolin derivatives. The major compound was identified as luteolin-pentosyl-hexoside. The antioxidant activities of the extract were moderate. The extract showed weak antibacterial activity against tested bacteria strains. Salmonella typhimurium ATCC 13311 was more sensitive to the extract than the others. The extract showed weak antiinflammatory activity.

 In this present study, the aerial parts of Verbascum eskisehirensis were dried in the shade and air-flowed area. The dried samples were macerated with 70 methanol in water for 24 h by three times. The biological activities of the extract was determined by in vitro methods. The antioxidant activity was evaluated in 2,2-diphenyl-1 picrylhydrazyl DPPH radical scavenging assay and the trolox equivalent antioxidant capacity TEAC was measured the ability of the extract to scavenge the stable radical cation ABTS 2,2-azinobis3-ethylbenzothiazoline-6-sulfonic acid. The antibacterial effects against some clinical pathogens were tested by broth microdilution assay. The in vitro antiinflammatory activity was also examined by 5-Lipoxygenase 5-LOX enzyme inhibitory test. In the same time, the phenolic composition of the extract was determined by LC-ESI-MSMS system. According to results, the extract was found rich-in luteolin derivatives. The major compound was identified as luteolin-pentosyl-hexoside. The antioxidant activities of the extract were moderate. The extract showed weak antibacterial activity against tested bacteria strains. Salmonella typhimurium ATCC 13311 was more sensitive to the extract than the others. The extract showed weak antiinflammatory activity.

Article Details

Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak & Ekici

Authors:

Gözde Öztürk | Hale Gamze Ağalar | Fatih Göğer

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.532-542

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347200

pISSN:

2630-6344

Verbascum eskisehirensis

luteolin

antioxidant

antimicrobial

antiinflammatory.

Issues

Article Details

Biological activities and luteolin derivatives of Verbascum eskisehirensis Karavel., Ocak & Ekici

Authors: Gözde Öztürk | Hale Gamze Ağalar | Fatih Göğer

Publisher: Marmara University

Main Topics: Pharmacognosy

Journal: Journal of Research in Pharmacy Vol.23 Issue 3, pp.532-542

Document Type: Article

Publication Date: 6/2025

Document Language: English

Document ID (Org No.): 2001347200

pISSN: 2630-6344

Verbascum eskisehirensis

luteolin

antioxidant

antimicrobial

antiinflammatory.

Issues

Authors:

Gözde Öztürk | Hale Gamze Ağalar | Fatih Göğer

Publisher:

Marmara University

Main Topics:

Pharmacognosy

Journal:

Journal of Research in Pharmacy Vol.23 Issue 3, pp.532-542

Document Type:

Article

Publication Date:

6/2025

Document Language:

English

Document ID (Org No.):

2001347200

pISSN:

2630-6344