Localization, Distribution and Structure of Muscle Fibres Using Specific Antibody Markers in the zebrafish, Danio rerio (Hamilton, 1822)

Hussein A.  Saud; Ilham J.J. Alshami; Ruth  Cooper

doi:10.37077/25200860.2021.34.1.13

Article Details

Localization, Distribution and Structure of Muscle Fibres Using Specific Antibody Markers in the zebrafish, Danio rerio (Hamilton, 1822)

Authors:

Hussein A. Saud | Ilham J.J. Alshami | Ruth Cooper

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

myosin

Journal:

Basrah Journal of Agricultural Sciences Vol.34 Issue 1, pp.147-155

Document Type:

Article

Publication Date:

2/2021

Document Language:

English

Document ID (Org No.):

2001363552

pISSN:

2520-0860

DOI:

10.37077/25200860.2021.34.1.13

Keywords:

Abstract:

 The zebrafish Danio rerio is a popular model species for genetic and early development studies. It is relatively easy to maintain in laboratory, has a high fecundity rate and produces transparent embryos. Here, we characterise muscle development in early life stage zebrafish using paraffin sections of embryos and larvae treated with haematoxylin and eosin staining, and whole mount fluorescent immunohistochemistry. We found variations in the distribution of muscle mass throughout the body, with the greatest proportion of muscle mass found in the tail. Our data also showed for the first time the reaction of antibodies (protein expression) in muscle at early life stages of development. Whole mount fluorescent immunohistochemistry staining with three markers (PAX7, MF20 and F59) suggests that muscle development starts even earlier than previously suggested at the embryonic stage (1 dpf).

Article Details

Localization, Distribution and Structure of Muscle Fibres Using Specific Antibody Markers in the zebrafish, Danio rerio (Hamilton, 1822)

Authors: Hussein A. Saud | Ilham J.J. Alshami | Ruth Cooper

Publisher: College of Agriculture, University of Basrah, Iraq

Main Topics: myosin

Journal: Basrah Journal of Agricultural Sciences Vol.34 Issue 1, pp.147-155

Document Type: Article

Publication Date: 2/2021

Document Language: English

Document ID (Org No.): 2001363552

pISSN: 2520-0860

DOI: 10.37077/25200860.2021.34.1.13

PAX7

MF20

F59

myosin

Issues

Authors:

Hussein A. Saud | Ilham J.J. Alshami | Ruth Cooper

Publisher:

College of Agriculture, University of Basrah, Iraq

Main Topics:

myosin

Journal:

Basrah Journal of Agricultural Sciences Vol.34 Issue 1, pp.147-155

Document Type:

Article

Publication Date:

2/2021

Document Language:

English

Document ID (Org No.):

2001363552

pISSN:

2520-0860

DOI:

10.37077/25200860.2021.34.1.13