In Vivo Study on the Role of Interferon-Γ and Interleukin-4 in Control of Cryptosporidiuminfection

Elagmy , Ahmed Sh.; Hassan , Khairy A.M.; Ali , Ibrahim R.; Abo-Sheishaa , Gamal A.

Article Details

In Vivo Study on the Role of Interferon-Γ and Interleukin-4 in Control of Cryptosporidiuminfection

Authors:

Elagmy , Ahmed Sh. | Hassan , Khairy A.M. | Ali , Ibrahim R. | Abo-Sheishaa , Gamal A.

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Medicine

Journal:

Journal of the Egyptian Society of Parasitology 2025 Vol.55 Issue 1, pp.93-102

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

273248

Frequency:

Tri-Annual

pISSN:

11100583

Descriptors:

Keywords:

Abstract:

 Cryptosporidiumspecies are protozoan parasites that cause severe diarrhea in immunocom-promised individuals and children, especially in developing countries. This study investigated the roles of IFN-γ and IL-4 in controlling Cryptosporidiuminfections in neonatal mice. Mice were grouped into four groups: normal control, infected control,infected/anti-IFN-γ mAb treated, and infected/anti-IL-4 mAb treated groups. Fecal assessments for C. parvumoocysts, cytokines detection, histopathological evaluations, and gene expression investigations for IL-4 and IFN-γ were conducted. The results showed a 22% mortality ratewasamong the infected anti-IFN-γ mAb-treated mice (11%) and anti-IL-4 mAb-treated ones (6%). Infected mice showed significantly higher IFN-γ levels than controls. Theanti-IFN-γ &anti-IL-4 treated onesraised serum cytokines with blocked biological activity bycompensatory responses. The increase in IFN-γ secretion in infected miceattemptedto overcome infection correlated with oocyst counts, histopathol-ogical, and gene expression changes. But, a negative correlation was between oocyst shedd-ing and IFN-γ levels in serum as well asintestinal expressionimplying the IFN-γ role in infe-ction control.Consequently, neutralization with anti-IL-4 mAb increased oocyst shedding early in cryptosporidiosis, clarifying the IL 4 rolein early infection control.

Article Details

In Vivo Study on the Role of Interferon-Γ and Interleukin-4 in Control of Cryptosporidiuminfection

Authors:

Elagmy , Ahmed Sh. | Hassan , Khairy A.M. | Ali , Ibrahim R. | Abo-Sheishaa , Gamal A.

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Medicine

Journal:

Journal of the Egyptian Society of Parasitology 2025 Vol.55 Issue 1, pp.93-102

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

273248

Frequency:

Tri-Annual

pISSN:

11100583

Descriptors:

LABORATORY EXPERIMENTS

MEDICAL RESEARCH

CHEMICALS

IMMUNITY

Cryptosporidium parvum

Controlling

IFN-γ

IL-4

RT-qPCR

Issues

Article Details

Authors: Elagmy , Ahmed Sh. | Hassan , Khairy A.M. | Ali , Ibrahim R. | Abo-Sheishaa , Gamal A.

Publisher: The Egyptian Society of Parasitology

Main Topics: Medicine

Journal: Journal of the Egyptian Society of Parasitology 2025 Vol.55 Issue 1, pp.93-102

Document Type: Article

Publication Date: 4/2025

Document Language: English

Document ID (Org No.): 273248

Frequency: Tri-Annual

pISSN: 11100583

Descriptors:

Issues

Authors:

Elagmy , Ahmed Sh. | Hassan , Khairy A.M. | Ali , Ibrahim R. | Abo-Sheishaa , Gamal A.

Publisher:

The Egyptian Society of Parasitology

Main Topics:

Medicine

Journal:

Journal of the Egyptian Society of Parasitology 2025 Vol.55 Issue 1, pp.93-102

Document Type:

Article

Publication Date:

4/2025

Document Language:

English

Document ID (Org No.):

273248

Frequency:

Tri-Annual

pISSN:

11100583