Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Deniz Cakar

Article Details

Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Authors:

Deniz Cakar

Publisher:

Duzce University

Main Topics:

Orman Entomolojisi ve Orman Koruma

Journal:

Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi Vol.20 Issue 2, pp.160-176

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001336354

pISSN:

2148-7871

Keywords:

Abstract:

 The stone pine Pinus pinea L. is a significant forest tree species in Türkiye. Its cultivation takes place in forest nurseries, and it is used in reforestation areas. The objective of this study to determine the causes of blight occurring in stone pine seedling in the Hendek Forest Nursery. Blight symptoms were observed on the needles of 1-2-years-old stone pine seedlings in the Hendek Forest Nursery. Isolates obtained as a result of isolation were identified as Neopestalotiopsis australis based on morphology and by combining sequence data from the LSU, ITS, tef1, and tub2 gene regions. A Maximum Likelihood phylogenetic tree was constructed for two isolates of N. australis based on four loci. Neopestalotiopsis australis was isolated from 25 out of 60 necrotic needles examined. The pathogenicity of N. australis was examined on healthy stone pine saplings by spraying spore suspension. After 28 days following the inoculation with spore suspensions, N. australis caused necrosis on needles. The pathogen was isolated from symptomatic needles in the stone pine the first time in the Türkiye. Neopestalotiopsis australis was proven to be a pathogen on stone pine. It has been concluded that N. australis could pose a threat to stone pine sapling. Further research should be conducted on this fungal pathogen for more detailed insights.

 The stone pine Pinus pinea L. is a significant forest tree species in Türkiye. Its cultivation takes place in forest nurseries, and it is used in reforestation areas. The objective of this study to determine the causes of blight occurring in stone pine seedling in the Hendek Forest Nursery. Blight symptoms were observed on the needles of 1-2-years-old stone pine seedlings in the Hendek Forest Nursery. Isolates obtained as a result of isolation were identified as Neopestalotiopsis australis based on morphology and by combining sequence data from the LSU, ITS, tef1, and tub2 gene regions. A Maximum Likelihood phylogenetic tree was constructed for two isolates of N. australis based on four loci. Neopestalotiopsis australis was isolated from 25 out of 60 necrotic needles examined. The pathogenicity of N. australis was examined on healthy stone pine saplings by spraying spore suspension. After 28 days following the inoculation with spore suspensions, N. australis caused necrosis on needles. The pathogen was isolated from symptomatic needles in the stone pine the first time in the Türkiye. Neopestalotiopsis australis was proven to be a pathogen on stone pine. It has been concluded that N. australis could pose a threat to stone pine sapling. Further research should be conducted on this fungal pathogen for more detailed insights.

 The stone pine Pinus pinea L. is a significant forest tree species in Türkiye. Its cultivation takes place in forest nurseries, and it is used in reforestation areas. The objective of this study to determine the causes of blight occurring in stone pine seedling in the Hendek Forest Nursery. Blight symptoms were observed on the needles of 1-2-years-old stone pine seedlings in the Hendek Forest Nursery. Isolates obtained as a result of isolation were identified as Neopestalotiopsis australis based on morphology and by combining sequence data from the LSU, ITS, tef1, and tub2 gene regions. A Maximum Likelihood phylogenetic tree was constructed for two isolates of N. australis based on four loci. Neopestalotiopsis australis was isolated from 25 out of 60 necrotic needles examined. The pathogenicity of N. australis was examined on healthy stone pine saplings by spraying spore suspension. After 28 days following the inoculation with spore suspensions, N. australis caused necrosis on needles. The pathogen was isolated from symptomatic needles in the stone pine the first time in the Türkiye. Neopestalotiopsis australis was proven to be a pathogen on stone pine. It has been concluded that N. australis could pose a threat to stone pine sapling. Further research should be conducted on this fungal pathogen for more detailed insights.

 Türkiyede fıstık çamı Pinus pinea L. önemli bir orman ağacıdır. Orman fidanlıklarında üretimi yapılmakta ve ağaçlandırma sahalarında kullanılmaktadır. Bu çalışmanın amacı, Hendek Orman Fidanlığında fıstık çamı fidanlarında tespit edilen kurumaların nedenlerini belirlemektir. Hendek Orman Fidanlığında 1-2 yaşlı fıstık çamı fidanlarının ibrelerinde kurumalar görülmüştür. Morfolojik ve LSU, ITS, tef1 ve tub2 gen bölgelerinden elde edilen sekans verileri kombinlenerek, izolasyon sonucunda elde edilen izolatlar Neopestalotiopsis australis olarak tanılanmıştır. Neopestalotiopsis australisin dört gen bölgesine dayalı iki izolatı için bir Maximum Likelihood filogenetik ağaç oluşturulmuştur. İncelenen 60 nekrotik ibrenin, 25 adedinden N. australis izole edilmiştir N. australisin patojenitesi sağlıklı fıstıkçamı fidanlarına spor süspansiyonunu püskürtme yöntemi kullanılarak test edilmiştir. Spor süspansiyonu uygulamasından 28 gün sonra, N. australis ibrelerde nekrozlara neden olmuştur. Patojen, Türkiyede fıstık çamı fidanlarında semptomatik ibrelerden ilk kez izole edilmişir. Fıstık çamı fidanlarında patojen olduğu tespit edilen Neopestalotiopsis australisin ciddi bir tehdit oluşturabileceği öngörülmektedir. Gelecek çalışmalarda bu fungal etmenle ilgili daha detaylı araştırmalar yapılması önerilmektedir.

Article Details

Role of Neopestalotiopsis australis in Needle Necrosis Blight of Stone Pine Saplings in Türkiye

Authors:

Deniz Cakar

Publisher:

Duzce University

Main Topics:

Orman Entomolojisi ve Orman Koruma

Journal:

Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi Vol.20 Issue 2, pp.160-176

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001336354

pISSN:

2148-7871

Stone pine

Fungus

Needle blight

Forest NurseryFıstık çamı

Fungus

İbre kurumaları

Orman Fidanlığı

Issues

Article Details

Authors: Deniz Cakar

Publisher: Duzce University

Main Topics: Orman Entomolojisi ve Orman Koruma

Journal: Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi Vol.20 Issue 2, pp.160-176

Document Type: Article

Publication Date: 12/2024

Document Language: English

Document ID (Org No.): 2001336354

pISSN: 2148-7871

Issues

Authors:

Deniz Cakar

Publisher:

Duzce University

Main Topics:

Orman Entomolojisi ve Orman Koruma

Journal:

Düzce Üniversitesi Orman Fakültesi Ormancılık Dergisi Vol.20 Issue 2, pp.160-176

Document Type:

Article

Publication Date:

12/2024

Document Language:

English

Document ID (Org No.):

2001336354

pISSN:

2148-7871