PCR-Based Study for Molecular Documentation of Human Herpes Virus Type – 8 and P15 Polymorphism in a Group of lymphoma Patients.

Ameer J. Abbas Al-Jawdhari; Shakir H. Mohammed Al-Alwany

doi:10.29196/jubpas.v31i3.5631

Article Details

PCR-Based Study for Molecular Documentation of Human Herpes Virus Type – 8 and P15 Polymorphism in a Group of lymphoma Patients.

Authors:

Ameer J. Abbas Al-Jawdhari | Shakir H. Mohammed Al-Alwany

Publisher:

University of Babylon

Journal:

JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.31 Issue 3, pp.324-337

Document Type:

Article

Publication Date:

9/2023

Document Language:

English

Document ID (Org No.):

2001383202

pISSN:

2312-8135

DOI:

10.29196/jubpas.v31i3.5631

Keywords:

Abstract:

 Background:
 Since its initial identification with primary effusion lymphoma (PEL), the range of lymphoproliferative illnesses associated with human herpesvirus 8 (HHV-8) infection has steadily expanded. Systemic B cell lymphoma often has p16(INK4a) and p15(INK4b) inactivation, which may indicate a poor prognosis. 200 specimens are studied cross-case-control. Lymphoma (LY) patients and normal people (control group) are sampled. Iraq's Middle Euphrates region's general hospitals and private clinics provided the samples. The study included 17-75-year-olds. Samples were collected between October 2022 and February 2023. PCR-based sequencing detected HHV-8 and p15 gene polymorphisms.
Materials and Methods:
 PCR was used to detect HHV-8 and P15 gene polymorphism in 100 patients and 100 healthy individuals. Conventional PCR sequenced HHV-8 and p15 gene polymorphisms
Results:
 31 out of 64 (48.4%) specimens tested positive for HHV-8, while 33 out of 64 (51.6%) tested negative. The age group (56-75 years) has 48% of HHV8-infected lymphomas, while the age groups (15-35 years) and (36-55 years) had 23% and 29%, respectively. The P15 polymorphism shows that the AA genotype increased lymphoma, while the TT genotype decreased it (p<0.05).
Conclusion: 
 Despite the small sample size, our findings suggest that HHV-8 and p15 gene polymorphism may affect lymphocyte tumor biology and development.

Article Details

PCR-Based Study for Molecular Documentation of Human Herpes Virus Type – 8 and P15 Polymorphism in a Group of lymphoma Patients.

Authors: Ameer J. Abbas Al-Jawdhari | Shakir H. Mohammed Al-Alwany

Publisher: University of Babylon

Journal: JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.31 Issue 3, pp.324-337

Document Type: Article

Publication Date: 9/2023

Document Language: English

Document ID (Org No.): 2001383202

pISSN: 2312-8135

DOI: 10.29196/jubpas.v31i3.5631

HHV-8

Lymphoma

PCR

P15

polymorphism

sequencing

Issues

Authors:

Ameer J. Abbas Al-Jawdhari | Shakir H. Mohammed Al-Alwany

Publisher:

University of Babylon

Journal:

JOURNAL OF UNIVERSITY OF BABYLON for Pure and Applied Sciences Vol.31 Issue 3, pp.324-337

Document Type:

Article

Publication Date:

9/2023

Document Language:

English

Document ID (Org No.):

2001383202

pISSN:

2312-8135

DOI:

10.29196/jubpas.v31i3.5631