Analysis and cloning of CP 1102 gene isolated from a medicinal plant

Hira Mubeen; Shahid Raza

doi:10.14419/ijbr.v5i1.6361

Article Details

Analysis and cloning of CP 1102 gene isolated from a medicinal plant

Authors:

Hira Mubeen | Shahid Raza

Publisher:

Science Publishing Corporation

Journal:

International Journal of Biological Research Vol.5 Issue 1, pp.26-29

Document Type:

Article

Publication Date:

12/2017

Document Language:

English

Document ID (Org No.):

2001387925

pISSN:

2307-9029

DOI:

10.14419/ijbr.v5i1.6361

Keywords:

Pdnor

Abstract:

 Calotropis procera is a common medicinal plant with various properties related with its latex which functions as a rich source of biologically active compounds. Latex is chemically diverse and the chemical and biochemical differences are considerable for different plant fluids. This plant can produce large quantity of latex. The study was performed to clone the CP1102 terminator region of gene in general expression vector PTZ57R/T. The objective was to make a variant of pJITT166 (size ~5.8kb) containing CP1102 terminator sequence to study different expression levels in future. The fragment of 341bp size was isolated. The pDNOR vector containing CP1102 terminator sequence was isolated and amplified by PCR. The forward and reverse primer specific to CP1102 terminator sequence which can amplify this sequence are designed by using bioinformatics tools.

Article Details

Analysis and cloning of CP 1102 gene isolated from a medicinal plant

Authors: Hira Mubeen | Shahid Raza

Publisher: Science Publishing Corporation

Journal: International Journal of Biological Research Vol.5 Issue 1, pp.26-29

Document Type: Article

Publication Date: 12/2017

Document Language: English

Document ID (Org No.): 2001387925

pISSN: 2307-9029

DOI: 10.14419/ijbr.v5i1.6361

Pdnor

Issues

Authors:

Hira Mubeen | Shahid Raza

Publisher:

Science Publishing Corporation

Journal:

International Journal of Biological Research Vol.5 Issue 1, pp.26-29

Document Type:

Article

Publication Date:

12/2017

Document Language:

English

Document ID (Org No.):

2001387925

pISSN:

2307-9029

DOI:

10.14419/ijbr.v5i1.6361