Glyceryl Trinitrate, a Vasodilating Drug Acts as an Antibiofilm Agent in Serratia marcescens

Ziyad Hameed Al-Fayyadh; Ahmed Mohammed Turki; Harith Al-Mathkhury

doi:10.22317/jcms.v9i3.1329

Article Details

Glyceryl Trinitrate, a Vasodilating Drug Acts as an Antibiofilm Agent in Serratia marcescens

Authors:

Ziyad Hameed Al-Fayyadh | Ahmed Mohammed Turki | Harith Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 3, pp.175-178

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001379550

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i3.1329

Keywords:

Abstract:

 Objectives: Serratia marcescens is a gram-negative pathogen of many species. The ability of S. marcescens to form biofilms and its potent innate resistance to antimicrobials and cleaning solutions are both essential for its pathogenicity and survival. The present study was conducted to investigate the effect of glyceryl trinitrate (GTN) on the biofilm of S. marcescens, as an alternative for antibiotic therapy.
Methods: Different specimens, including ear swabs, burns, mid-stream urine, wound swabs, and sputum, were collected from patients who were brought to Al-Ramadi Hospital, Iraq. All samples were cultured, and the colonies that were obtained were detected using the VITEK® 2 compact. The ability of biofilms to develop was examined using the microtiter plate technique. The bactericidal effectiveness of GTN was estimated by the broth microdilution technique. The presence of fimA and fimC in S. marcescens isolates was detected using the polymerase chain reaction (PCR) method. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to assess the effect of GTN on fimA and fimC gene expression. 
Results: The results demonstrated that GTN has no effect on S. marcescens growth; while its biofilm was significantly (p<0.05) influenced. Moreover, all S. marcescens isolates had fimA and fimC, and the presence of GTN reduced the expression of these genes.
Conclusion: The findings of this study reveal that GTN can act as a promising antibiofilm agent in reference to S. marcescens.

Article Details

Glyceryl Trinitrate, a Vasodilating Drug Acts as an Antibiofilm Agent in Serratia marcescens

Authors:

Ziyad Hameed Al-Fayyadh | Ahmed Mohammed Turki | Harith Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 3, pp.175-178

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001379550

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i3.1329

fimA

fimC

Glyceryl trinitrate

Serratia marcescens

Issues

Article Details

Glyceryl Trinitrate, a Vasodilating Drug Acts as an Antibiofilm Agent in Serratia marcescens

Authors: Ziyad Hameed Al-Fayyadh | Ahmed Mohammed Turki | Harith Al-Mathkhury

Publisher: Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal: Journal of Contemporary Medical Sciences Vol.9 Issue 3, pp.175-178

Document Type: Article

Publication Date: 6/2023

Document Language: English

Document ID (Org No.): 2001379550

pISSN: 2413-0516

DOI: 10.22317/jcms.v9i3.1329

fimA

fimC

Glyceryl trinitrate

Serratia marcescens

Issues

Authors:

Ziyad Hameed Al-Fayyadh | Ahmed Mohammed Turki | Harith Al-Mathkhury

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.9 Issue 3, pp.175-178

Document Type:

Article

Publication Date:

6/2023

Document Language:

English

Document ID (Org No.):

2001379550

pISSN:

2413-0516

DOI:

10.22317/jcms.v9i3.1329