Evaluation of the Chromogenic Medium ( CPS ) in the Isolation and Identification of Urinary Tract Pathogens

Reham Raafat; Reham Dwedar; Eman El-Seidi

doi:10.12816/0026084

Article Details

Evaluation of the Chromogenic Medium ( CPS ) in the Isolation and Identification of Urinary Tract Pathogens

Authors:

Reham Raafat | Reham Dwedar | Eman El-Seidi

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2015 Vol.24 Issue 2, pp.29-34

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

86493

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0026084

Descriptors:

Keywords:

Abstract:

 Background: Urine cultures constitute the majority of the workload for a microbiologylaboratory with only 20%-30% of urine sample resulting in significant growth.Chromogenic media (CM) are available for urine specimens to enable rapididentification of common pathogens and also has been reported to increase mixedculture detection, reducing unnecessary workup. Chromogenic media offers the potentialto lower costs by providing decreased work time, storage space and identification costs.Objectives: The present study focused on evaluation of the chromogenic medium (CPS)for the diagnosis of UTI in comparison with CLED as a conventional medium.Methodology: Over the period of January to July 2014, fifty urine samples with ≥100pus cells /HPF were examined. CPS and CLED media were used for direct inoculationin addition to conventional biochemical reactions and/or API as needed. Results: Incomparison with CLED, CPS showed a sensitivity of 93.5%, specificity of 100%, positivepredictive value of 100%, negative predictive value of 57.1% and total agreement of94%. The sensitivity of CPS for E. coli was 95%, for KESC was 75%, for Proteeae was100% and for Enterococcus was 100%. The specificity of CPS for detection of E. coliwas 100%, for KESC was 100%, for Proteeae was 97.9% and for Enterococcus was100%. Conclusion: CPS proved to be a rapid, cost-effective diagnostic method forurinary tract infections. Therefore, CPS can replace the standard primary plating mediaused in routine diagnosis of urinary tract infection.

Article Details

Evaluation of the Chromogenic Medium ( CPS ) in the Isolation and Identification of Urinary Tract Pathogens

Authors:

Reham Raafat | Reham Dwedar | Eman El-Seidi

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2015 Vol.24 Issue 2, pp.29-34

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

86493

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0026084

Descriptors:

URINARY SYSTEM DISEASES

URINARY TRACT DISEASES

URINE ANALYSIS

DIAGNOSIS

THERAPY

Urinary tract infection

Chromogenic media

Issues

Article Details

Authors: Reham Raafat | Reham Dwedar | Eman El-Seidi

Publisher: The Egyptian Society of Medical Microbiology

Main Topics: Medicine

Journal: The Egyptian Journal of Medical Microbiology 2015 Vol.24 Issue 2, pp.29-34

Document Type: Article

Publication Date: 4/2015

Document Language: English

Document ID (Org No.): 86493

Frequency: Quarterly

pISSN: 11102179

DOI: 10.12816/0026084

Descriptors:

Issues

Authors:

Reham Raafat | Reham Dwedar | Eman El-Seidi

Publisher:

The Egyptian Society of Medical Microbiology

Main Topics:

Medicine

Journal:

The Egyptian Journal of Medical Microbiology 2015 Vol.24 Issue 2, pp.29-34

Document Type:

Article

Publication Date:

4/2015

Document Language:

English

Document ID (Org No.):

86493

Frequency:

Quarterly

pISSN:

11102179

DOI:

10.12816/0026084