Detection of BRAF Gene in Some Iraqi Bowel Inflammation and Colorectal Cancer Patients

Ibtisam Hammood Naser AL Musawi; Wafaa Sabri Mahood; Mohammed Mahdi Jawad; Asmaa Ali Jawad

doi:10.30526/30.1.1054

Article Details

Detection of BRAF Gene in Some Iraqi Bowel Inflammation and Colorectal Cancer Patients

Authors:

Ibtisam Hammood Naser AL Musawi | Wafaa Sabri Mahood | Mohammed Mahdi Jawad | Asmaa Ali Jawad

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.30 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

8/2017

Document Language:

English

Document ID (Org No.):

2001377536

pISSN:

2521-3407

DOI:

10.30526/30.1.1054

Keywords:

Abstract:

 The impacts of the inflammatory process on neoplasia development were observed in many cancer, it has a great role in the etiology, development and progression of invasive colorectal tumors. This study was designed to investigate the BRAF mutation and assist the clinicopathological parameter in some Iraqi bowel inflammation and colorectal cancer patients. Thirty patients were enrolled in this study (15 suffering bowel inflammation and 15 having colorectal cancer). BRAF gene was screened for the presence of mutations using PCR technique and direct  sequencing. .The results revealed no BRAF mutation in position 1799 for exon fifteen in both samples of bowel inflammation and colorectal cancer. These results were confirmed previous articles regarding low rate of BRAF gene mutation in Asian countries. These results indicate the possibility of colorectal patients treatment with monoclonal antibodies. Several heterogeneity mutations were found in 3 out of 30 patients (10%) including transition two mutations G>A and one mutation was transversion mutation A>T in different sites as silent mutations.

Article Details

Detection of BRAF Gene in Some Iraqi Bowel Inflammation and Colorectal Cancer Patients

Authors:

Ibtisam Hammood Naser AL Musawi | Wafaa Sabri Mahood | Mohammed Mahdi Jawad | Asmaa Ali Jawad

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.30 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

8/2017

Document Language:

English

Document ID (Org No.):

2001377536

pISSN:

2521-3407

DOI:

10.30526/30.1.1054

BRAFV600E

bowel inflammation

colorectal

Iraq

heterogeneity

Issues

Article Details

Detection of BRAF Gene in Some Iraqi Bowel Inflammation and Colorectal Cancer Patients

Authors: Ibtisam Hammood Naser AL Musawi | Wafaa Sabri Mahood | Mohammed Mahdi Jawad | Asmaa Ali Jawad

Publisher: University of Baghdad

Journal: Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.30 Issue 1, pp.1-10

Document Type: Article

Publication Date: 8/2017

Document Language: English

Document ID (Org No.): 2001377536

pISSN: 2521-3407

DOI: 10.30526/30.1.1054

BRAFV600E

bowel inflammation

colorectal

Iraq

heterogeneity

Issues

Authors:

Ibtisam Hammood Naser AL Musawi | Wafaa Sabri Mahood | Mohammed Mahdi Jawad | Asmaa Ali Jawad

Publisher:

University of Baghdad

Journal:

Ibn AL-Haitham Journal For Pure and Applied Sciences Vol.30 Issue 1, pp.1-10

Document Type:

Article

Publication Date:

8/2017

Document Language:

English

Document ID (Org No.):

2001377536

pISSN:

2521-3407

DOI:

10.30526/30.1.1054