DEVELOPMENT AND VALIDATION OF A NEW UHPLC-DAD APPROACH FOR ATOMOXETINE DETECTION IN SEVERAL MEDICINAL PLANTS

Burhan Ceylan

Article Details

DEVELOPMENT AND VALIDATION OF A NEW UHPLC-DAD APPROACH FOR ATOMOXETINE DETECTION IN SEVERAL MEDICINAL PLANTS

Authors:

Burhan Ceylan

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya | Eczacılık ve İlaç Bilimleri (Diğer)

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.189-196

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300949

pISSN:

1015-3918

Keywords:

Abstract:

 Objective: Atomoxetine ATX is a medication that is extensively used to treat attention deficit hyperactivity disorder in children, adolescents, and adults. The goal of this work was to create a speedy, easy, and sensitive ultra high performance liquid chromatographic method UHPLC for the measurement of atomoxetine in various medicinal plants. Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L.. Material and Method: Prior to chromatographic separation, liquid-liquid extraction was applied, which is currently the preferred extraction technique due to its simple, fast and efficient procedure for sample preparation. The chromatographic separation was achieved by reversed phase C18 5 μm  4.6 mm  150 mm analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate pH6.8 and acetonitrile 50:50 vv at flow rate of 0.8 mlmin and diode array detector DAD detecting at 2152 nm. Result and Discussion: The envisioned methods linear behavior was tested in the 0.5-20 μgml range r20.09990. In compliance with International Conference on Harmonisation ICH criteria, the method received validation by means of accuracy, precision, repeatability, specificity, robustness, and detection and quantification boundaries. LOD and LOQ values were determined as 0.16 and 0.5 μgml. RSD values for hourly and daily measurements are found to be below 2.5 for both assays. The proposed method can be used effectively for quantification of atomoxetine in medicinal and aromatic plants. The proposed analytical procedure represents an efficient method for the quantification and routinee analysis of atomoxetine in medicinal and aromatic plants.

 Objective: Atomoxetine ATX is a medication that is extensively used to treat attention deficit hyperactivity disorder in children, adolescents, and adults. The goal of this work was to create a speedy, easy, and sensitive ultra high performance liquid chromatographic method UHPLC for the measurement of atomoxetine in various medicinal plants. Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L.. Material and Method: Prior to chromatographic separation, liquid-liquid extraction was applied, which is currently the preferred extraction technique due to its simple, fast and efficient procedure for sample preparation. The chromatographic separation was achieved by reversed phase C18 5 μm  4.6 mm  150 mm analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate pH6.8 and acetonitrile 50:50 vv at flow rate of 0.8 mlmin and diode array detector DAD detecting at 2152 nm. Result and Discussion: The envisioned methods linear behavior was tested in the 0.5-20 μgml range r20.09990. In compliance with International Conference on Harmonisation ICH criteria, the method received validation by means of accuracy, precision, repeatability, specificity, robustness, and detection and quantification boundaries. LOD and LOQ values were determined as 0.16 and 0.5 μgml. RSD values for hourly and daily measurements are found to be below 2.5 for both assays. The proposed method can be used effectively for quantification of atomoxetine in medicinal and aromatic plants. The proposed analytical procedure represents an efficient method for the quantification and routinee analysis of atomoxetine in medicinal and aromatic plants.

 Objective: Atomoxetine ATX is a medication that is extensively used to treat attention deficit hyperactivity disorder in children, adolescents, and adults. The goal of this work was to create a speedy, easy, and sensitive ultra high performance liquid chromatographic method UHPLC for the measurement of atomoxetine in various medicinal plants. Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L.. Material and Method: Prior to chromatographic separation, liquid-liquid extraction was applied, which is currently the preferred extraction technique due to its simple, fast and efficient procedure for sample preparation. The chromatographic separation was achieved by reversed phase C18 5 μm  4.6 mm  150 mm analytical column and a mobile phase consisting of monobasic potassium dihydrogen orthophosphate pH6.8 and acetonitrile 50:50 vv at flow rate of 0.8 mlmin and diode array detector DAD detecting at 2152 nm. Result and Discussion: The envisioned methods linear behavior was tested in the 0.5-20 μgml range r20.09990. In compliance with International Conference on Harmonisation ICH criteria, the method received validation by means of accuracy, precision, repeatability, specificity, robustness, and detection and quantification boundaries. LOD and LOQ values were determined as 0.16 and 0.5 μgml. RSD values for hourly and daily measurements are found to be below 2.5 for both assays. The proposed method can be used effectively for quantification of atomoxetine in medicinal and aromatic plants. The proposed analytical procedure represents an efficient method for the quantification and routinee analysis of atomoxetine in medicinal and aromatic plants.

 Amaç: Atomoksetin ATX çocuklarda, ergenlerde ve yetişkinlerde dikkat eksikliği hiperaktivite bozukluğunun tedavisinde yaygın olarak kullanılmaktadır. Bu çalışmada, bazı tıbbi bitkilerde Salvia officinalis L., Rosmarinus officinalis L., Melissa officinalis L., Ginkgo biloba L. atomoksetin analizi için hızlı, basit ve hassas bir ultra yüksek performanslı sıvı kromatografik yöntem UHPLC geliştirilmesi amaçlanmıştır. Gereç ve Yöntem: Kromatografik ayırmadan önce, numune hazırlama için basit, hızlı ve verimli prosedürü nedeniyle günümüzde tercih edilen ekstraksiyon tekniği olan sıvı-sıvı ekstraksiyonu uygulanmıştır. Kromatografik ayırma, ters fazlı C18 5 μm  4.6 mm  150 mm analitik kolon ve monobazik potasyum dihidrojen ortofosfat pH6.8 ve asetonitrilden 50:50 hh oluşan bir mobil fazda 0.8 mldk akış hızında ve 2152 nmde diyot dizisi dedektörü DAD tespiti ile sağlandı. Sonuç ve Tartışma: Önerilen yöntemin doğrusallığı 0.5-20 μgml r20.9990 aralığında incelenmiştir. Yöntem, Uluslararası Uyumlaştırma Konferansı ICH yönergelerine uygun olarak doğruluk, kesinlik, tekrarlanabilirlik, özgüllük, sağlamlık ve dedeksiyon ve kantitasyon limitleri açısından doğrulanmıştır. LOD ve LOQ sırasıyla 0.16 ve 0.5 μgml olarak bulundu. Gün içi ve günler arası RSD değerleri her iki test için de 2.5in altındadır. Önerilen yöntem, tıbbi ve aromatik bitkilerde atomoksetin miktarının belirlenmesi için etkin bir şekilde kullanılabilir. Önerilen analitik prosedür, tıbbi ve aromatik bitkilerde atomoksetinin miktarının belirlenmesi ve rutin analizi için etkili bir yöntemi temsil eder.

Article Details

DEVELOPMENT AND VALIDATION OF A NEW UHPLC-DAD APPROACH FOR ATOMOXETINE DETECTION IN SEVERAL MEDICINAL PLANTS

Authors:

Burhan Ceylan

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya | Eczacılık ve İlaç Bilimleri (Diğer)

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.189-196

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300949

pISSN:

1015-3918

Atomoxetine

attention deficit hyperactivity disorder

diode array detector (DAD)

medicinal plants

ultra high performance liquid chromatography (UHPLC)Atomoksetin

dikkat eksikliği hiperaktivite bozukluğu

diyot dizisi dedektörü (DAD)

tıbbi bitkiler

ultra yüksek performanslı sıvı kromatografisi (UHPLC)

Issues

Article Details

Authors: Burhan Ceylan

Publisher: Ankara University

Main Topics: Eczacılıkta Analitik Kimya | Eczacılık ve İlaç Bilimleri (Diğer)

Journal: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.189-196

Document Type: Article

Publication Date: 1/2024

Document Language: English

Document ID (Org No.): 2001300949

pISSN: 1015-3918

Issues

Authors:

Burhan Ceylan

Publisher:

Ankara University

Main Topics:

Eczacılıkta Analitik Kimya | Eczacılık ve İlaç Bilimleri (Diğer)

Journal:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.48 Issue 1, pp.189-196

Document Type:

Article

Publication Date:

1/2024

Document Language:

English

Document ID (Org No.):

2001300949

pISSN:

1015-3918