Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

Behiye Banu BİLGEN; Nuray KAYA

Article Details

Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

Authors:

Behiye Banu BİLGEN | Nuray KAYA

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 4, pp.540-549

Document Type:

Article

Publication Date:

3/2014

Document Language:

Turkish

Document ID (Org No.):

2000807354

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Keywords:

Abstract:

 

Pollen contamination is one of the important factors affecting the yield, adaptability, and genetic quality of the seed produced from seed orchards in forest tree breeding programs. Incoming pollen from the forests surrounding the seed orchard is a major concern in tree breeding because it contributes to losses in the expected genetic gains from seed orchard crops. The genetic variation and the level of pollen contamination in a 16-year-old Pinus brutia Ten. first-generation clonal seed orchard was studied using chloroplast microsatellite markers (cpSSRs). In total, 23 alleles and 36 unique allelic combinations (haplotypes) were detected based on the 6 cpSSR loci analyzed. The haplotypic diversity of the clones in the seed orchard was found to be 0.849. Out of 300 embryos analyzed, 87 were not compatible with any male parent within the seed orchard. Thus, 29% of the embryos were sired by pollen sources outside the orchard (i.e. apparent contamination). Microsatellite-based analysis revealed that the estimated contamination rate was 39.3%. Background pollination at this level will cause losses of 20% in the expected genetic gains. Our findings are valuable for the assessment of the intended seed orchard function, i.e. provision of genetically improved seed. It may be worthwhile to use pollen management strategies like strobilus stimulation, controlled pollination, and supplemental mass pollination to decrease pollen contamination and increase the genetic quality of the seeds produced.

 

Pollen contamination is one of the important factors affecting the yield, adaptability, and genetic quality of the seed produced from seed orchards in forest tree breeding programs. Incoming pollen from the forests surrounding the seed orchard is a major concern in tree breeding because it contributes to losses in the expected genetic gains from seed orchard crops. The genetic variation and the level of pollen contamination in a 16-year-old Pinus brutia Ten. first-generation clonal seed orchard was studied using chloroplast microsatellite markers (cpSSRs). In total, 23 alleles and 36 unique allelic combinations (haplotypes) were detected based on the 6 cpSSR loci analyzed. The haplotypic diversity of the clones in the seed orchard was found to be 0.849. Out of 300 embryos analyzed, 87 were not compatible with any male parent within the seed orchard. Thus, 29% of the embryos were sired by pollen sources outside the orchard (i.e. apparent contamination). Microsatellite-based analysis revealed that the estimated contamination rate was 39.3%. Background pollination at this level will cause losses of 20% in the expected genetic gains. Our findings are valuable for the assessment of the intended seed orchard function, i.e. provision of genetically improved seed. It may be worthwhile to use pollen management strategies like strobilus stimulation, controlled pollination, and supplemental mass pollination to decrease pollen contamination and increase the genetic quality of the seeds produced.

Article Details

Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

Authors:

Behiye Banu BİLGEN | Nuray KAYA

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 4, pp.540-549

Document Type:

Article

Publication Date:

3/2014

Document Language:

Turkish

Document ID (Org No.):

2000807354

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X

Chloroplast microsatellite locus

genetic markers

gene flow

clonal seed orchard

Turkish red pine

Issues

Article Details

Chloroplast DNA variation and pollen contamination in a Pinus brutia Ten. clonal seed orchard: implication for progeny performance in plantations

Authors: Behiye Banu BİLGEN | Nuray KAYA

Publisher: TUBITAK

Journal: Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 4, pp.540-549

Document Type: Article

Publication Date: 3/2014

Document Language: Turkish

Document ID (Org No.): 2000807354

Frequency: Bimonthly

eISSN: 1303-6173

pISSN: 1300-011X

Chloroplast microsatellite locus

genetic markers

gene flow

clonal seed orchard

Turkish red pine

Issues

Authors:

Behiye Banu BİLGEN | Nuray KAYA

Publisher:

TUBITAK

Journal:

Turkish Journal of Agriculture and Forestry 2014 Vol.38 Issue 4, pp.540-549

Document Type:

Article

Publication Date:

3/2014

Document Language:

Turkish

Document ID (Org No.):

2000807354

Frequency:

Bimonthly

eISSN:

1303-6173

pISSN:

1300-011X