Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Muhammad AASIM; Khalid Mahmood KHAWAR; Sebahattin ÖZCAN

Article Details

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Authors:

Muhammad AASIM | Khalid Mahmood KHAWAR | Sebahattin ÖZCAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.472-478

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812411

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Keywords:

Abstract:

 

Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement. The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz. The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25-0.75 mg L-1 6-benzylaminopurine with or without 0.25 mg L-1 a-naphthalene acetic acid. Shoot regeneration varied 44.4%-83.3% with 2.1-5.0 shoots per explant. Regenerated shoots were rooted in MS medium containing 0.50 mg L-1 indole-3-butyric acid and acclimatized in the greenhouse, where they flowered and set seeds. Immature cotyledons were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant binary vector pRGG containing an herbicide tolerance gene (bar) along with a uida (GUS) gene under 35S promoter. Phosphinothricin was used as a selectable marker at a concentration of 2.5 mg L-1. Putative transformants were screened by the histochemical GUS assay. Furthermore, molecular analysis revealed the presence of the introduced gene in the genome of cowpea cultivar Akkiz. The selected transgenic plants showed a resistance to Basta® nonselective herbicide at up to 10 mL L-1 of water. Putative transgenic plants retained their pigmentation and continued to grow in the greenhouse.

 

Plant genetic engineering has opened new avenues to modify crops and has provided a powerful tool for crop improvement. The present study reports the development of regeneration and genetic transformation protocol for the Turkish cowpea cultivar Akkiz. The immature cotyledons were cultured on Murashige and Skoog (MS) medium containing 0.25-0.75 mg L-1 6-benzylaminopurine with or without 0.25 mg L-1 a-naphthalene acetic acid. Shoot regeneration varied 44.4%-83.3% with 2.1-5.0 shoots per explant. Regenerated shoots were rooted in MS medium containing 0.50 mg L-1 indole-3-butyric acid and acclimatized in the greenhouse, where they flowered and set seeds. Immature cotyledons were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant binary vector pRGG containing an herbicide tolerance gene (bar) along with a uida (GUS) gene under 35S promoter. Phosphinothricin was used as a selectable marker at a concentration of 2.5 mg L-1. Putative transformants were screened by the histochemical GUS assay. Furthermore, molecular analysis revealed the presence of the introduced gene in the genome of cowpea cultivar Akkiz. The selected transgenic plants showed a resistance to Basta® nonselective herbicide at up to 10 mL L-1 of water. Putative transgenic plants retained their pigmentation and continued to grow in the greenhouse.

Article Details

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Authors:

Muhammad AASIM | Khalid Mahmood KHAWAR | Sebahattin ÖZCAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.472-478

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812411

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152

Key words: Regeneration

herbicide tolerance

genome

Issues

Article Details

Production of herbicide-resistant cowpea (Vigna unguiculata L.) transformed with the bar gene

Authors: Muhammad AASIM | Khalid Mahmood KHAWAR | Sebahattin ÖZCAN

Publisher: TUBITAK

Journal: Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.472-478

Document Type: Article

Publication Date: 3/2013

Document Language: Turkish

Document ID (Org No.): 2000812411

Frequency: Bimonthly

eISSN: 1303-6092

pISSN: 1300-0152

Key words: Regeneration

herbicide tolerance

genome

Issues

Authors:

Muhammad AASIM | Khalid Mahmood KHAWAR | Sebahattin ÖZCAN

Publisher:

TUBITAK

Journal:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.472-478

Document Type:

Article

Publication Date:

3/2013

Document Language:

Turkish

Document ID (Org No.):

2000812411

Frequency:

Bimonthly

eISSN:

1303-6092

pISSN:

1300-0152