The Relation Between Low-Density Lipoprotein (LDL) Oxidation and Hemodialysis with Respect to Membrane Types Protective Effects of Vitamin E-bonded membrane

Selda DEMİRTAŞ

Article Details

The Relation Between Low-Density Lipoprotein (LDL) Oxidation and Hemodialysis with Respect to Membrane Types Protective Effects of Vitamin E-bonded membrane

Authors:

Selda DEMİRTAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 2, pp.93-100

Document Type:

Article

Publication Date:

1/2002

Document Language:

Turkish

Document ID (Org No.):

2000757018

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Keywords:

Abstract:

 

Plasma total antioxidant (TAO) levels, LDL (low density lipoprotein), antioxidant defense potential (AOP) and the resistance to oxidation of LDL fractions were studied in chronic hemodialysis patients. The differences with respect to dialyzer membrane types before and after hemodialysis  were also assessed. After isolation of LDL from plasma by precipitation, AOP was measured based on the determination of TBARS (thiobarbituric acid reactive substance), and resistance to oxidation was assessed by the metal-catalyzed peroxidation of LDL followed by the determination of the values of the peroxidation product malondialdehide (MDA) before and after peroxidation. The assay principle of  TAO was based on the  suppression of ABTS+ radical cation (2,2'-Azino-di- [3-ethylbenzthiazoline sulfonate]) by the sample. AOP and the resistance to oxidation  of LDL were expressed as 1/nmol/ ml.h. Plasma TAO levels of patients (n=22) were significantly lower than those of the control group (n=20). In the patient group, the decrease in TAO levels  was also significant and continued to the end of the single dialysis session. The rate of decrease was similar in the two types of conventional membranes used (polysulfone  and hemophan). The antioxidant defense potential of LDL significantly decreased after hemodialysis  with the polysulfone type dialyzer but resistance to oxidation  values of LDL showed no difference. When the same group of patients were hemodialyzed with a vitamin E bonded membrane no difference was observed in the AOP of LDL, but resistance to oxidation of the LDL fraction significantly increased during the hemodialysis session. The results suggest that the vitamin E-bonded dialysis membrane strengthens the LDL antioxidant defense potential  and protects the LDL from oxidation.   In conclusion, the decreased  antioxidant capacity in these patients may be attributed to several factors such as elimination via the dialyzer of enzymatic or nonenzymatic antioxidants or deformation of these compounds by the increased oxidant stress. Improving LDL resistance to oxidation by using a vitamin E-bonded membrane appears to be an attractive alternative to prevent accelerated oxidative processes in hemodialysis patients.

 

Plasma total antioxidant (TAO) levels, LDL (low density lipoprotein), antioxidant defense potential (AOP) and the resistance to oxidation of LDL fractions were studied in chronic hemodialysis patients. The differences with respect to dialyzer membrane types before and after hemodialysis  were also assessed. After isolation of LDL from plasma by precipitation, AOP was measured based on the determination of TBARS (thiobarbituric acid reactive substance), and resistance to oxidation was assessed by the metal-catalyzed peroxidation of LDL followed by the determination of the values of the peroxidation product malondialdehide (MDA) before and after peroxidation. The assay principle of  TAO was based on the  suppression of ABTS+ radical cation (2,2'-Azino-di- [3-ethylbenzthiazoline sulfonate]) by the sample. AOP and the resistance to oxidation  of LDL were expressed as 1/nmol/ ml.h. Plasma TAO levels of patients (n=22) were significantly lower than those of the control group (n=20). In the patient group, the decrease in TAO levels  was also significant and continued to the end of the single dialysis session. The rate of decrease was similar in the two types of conventional membranes used (polysulfone  and hemophan). The antioxidant defense potential of LDL significantly decreased after hemodialysis  with the polysulfone type dialyzer but resistance to oxidation  values of LDL showed no difference. When the same group of patients were hemodialyzed with a vitamin E bonded membrane no difference was observed in the AOP of LDL, but resistance to oxidation of the LDL fraction significantly increased during the hemodialysis session. The results suggest that the vitamin E-bonded dialysis membrane strengthens the LDL antioxidant defense potential  and protects the LDL from oxidation.   In conclusion, the decreased  antioxidant capacity in these patients may be attributed to several factors such as elimination via the dialyzer of enzymatic or nonenzymatic antioxidants or deformation of these compounds by the increased oxidant stress. Improving LDL resistance to oxidation by using a vitamin E-bonded membrane appears to be an attractive alternative to prevent accelerated oxidative processes in hemodialysis patients.

Article Details

The Relation Between Low-Density Lipoprotein (LDL) Oxidation and Hemodialysis with Respect to Membrane Types Protective Effects of Vitamin E-bonded membrane

Authors:

Selda DEMİRTAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 2, pp.93-100

Document Type:

Article

Publication Date:

1/2002

Document Language:

Turkish

Document ID (Org No.):

2000757018

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144

Oxidative stress

hemodialysis

antioxidants

LDL oxidation

Issues

Article Details

The Relation Between Low-Density Lipoprotein (LDL) Oxidation and Hemodialysis with Respect to Membrane Types Protective Effects of Vitamin E-bonded membrane

Authors: Selda DEMİRTAŞ

Publisher: TUBITAK

Journal: Turkish Journal of Medical Sciences 2002 Vol.32 Issue 2, pp.93-100

Document Type: Article

Publication Date: 1/2002

Document Language: Turkish

Document ID (Org No.): 2000757018

Frequency: Bimonthly

eISSN: 1303-6165

pISSN: 1300-0144

Oxidative stress

hemodialysis

antioxidants

LDL oxidation

Issues

Authors:

Selda DEMİRTAŞ

Publisher:

TUBITAK

Journal:

Turkish Journal of Medical Sciences 2002 Vol.32 Issue 2, pp.93-100

Document Type:

Article

Publication Date:

1/2002

Document Language:

Turkish

Document ID (Org No.):

2000757018

Frequency:

Bimonthly

eISSN:

1303-6165

pISSN:

1300-0144