In Vitro Antagonistic Effects of Endophytic Fungi Isolated from Grapevine Rootstocks and Cultivars Against Some of the Fungal Pathogens Associated with Grapevine Trunk Diseases

Arife Yağcı; Davut Soner Akgül; Rüstem Cangi

Article Details

In Vitro Antagonistic Effects of Endophytic Fungi Isolated from Grapevine Rootstocks and Cultivars Against Some of the Fungal Pathogens Associated with Grapevine Trunk Diseases

Authors:

Arife Yağcı | Davut Soner Akgül | Rüstem Cangi

Publisher:

Cukurova University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.451-461

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001337447

pISSN:

2636-7874

Keywords:

Abstract:

 This study was carried out to determine the in vitro antagonistic effects of endophytic fungi obtained from grapevine cultivars and rootstocks grown in Tokat conditions against pathogenic fungi associated with grapevine trunk diseases. The dual culture method was followed on a PDA potato dextrose agar medium to reveal the antagonistic effects, and the distances in inhibition zones between the isolates were measured. The pathogenicity of the fungal isolates was tested on dormant canes of the Narince grape cultivars, and the isolates were inoculated using the top inoculation method. Inoculated cuttings were planted in 1-liter pots and grown at 25C and 85 relative humidity for three months. Inhibition distances of 0-13.3 mm between endophytic fungi and Botryosphaeria dothidea, 0-6.3 mm between Cadophora lutea-olivacea, 0-0.7 mm between Cytospora salicacearum, 0-5.0 mm between Diatrype stigma, and 0-8.7 mm between Truncatella angustata occurred. No endophyte inhibited all pathogens to the same extent, with solid effects against some pathogens and weak effects against others. When the inhibition performance of all endophytes was compared with each other, Fusarium avenaceum was the most effective, while Penicillium commune was found to be completely ineffective. Endophytic species did not cause significant necrosis in pathogenicity tests, while B. dothidea, C. lutea-olivacea, C. salicacearum, D. stigma and T. angustata caused necroses of 23.6-65.0 mm. This study indicates that different endophytes in grapevine may be promising for biologically controlling pathogens associated with grapevine trunk diseases.

 This study was carried out to determine the in vitro antagonistic effects of endophytic fungi obtained from grapevine cultivars and rootstocks grown in Tokat conditions against pathogenic fungi associated with grapevine trunk diseases. The dual culture method was followed on a PDA potato dextrose agar medium to reveal the antagonistic effects, and the distances in inhibition zones between the isolates were measured. The pathogenicity of the fungal isolates was tested on dormant canes of the Narince grape cultivars, and the isolates were inoculated using the top inoculation method. Inoculated cuttings were planted in 1-liter pots and grown at 25C and 85 relative humidity for three months. Inhibition distances of 0-13.3 mm between endophytic fungi and Botryosphaeria dothidea, 0-6.3 mm between Cadophora lutea-olivacea, 0-0.7 mm between Cytospora salicacearum, 0-5.0 mm between Diatrype stigma, and 0-8.7 mm between Truncatella angustata occurred. No endophyte inhibited all pathogens to the same extent, with solid effects against some pathogens and weak effects against others. When the inhibition performance of all endophytes was compared with each other, Fusarium avenaceum was the most effective, while Penicillium commune was found to be completely ineffective. Endophytic species did not cause significant necrosis in pathogenicity tests, while B. dothidea, C. lutea-olivacea, C. salicacearum, D. stigma and T. angustata caused necroses of 23.6-65.0 mm. This study indicates that different endophytes in grapevine may be promising for biologically controlling pathogens associated with grapevine trunk diseases.

 This study was carried out to determine the in vitro antagonistic effects of endophytic fungi obtained from grapevine cultivars and rootstocks grown in Tokat conditions against pathogenic fungi associated with grapevine trunk diseases. The dual culture method was followed on a PDA potato dextrose agar medium to reveal the antagonistic effects, and the distances in inhibition zones between the isolates were measured. The pathogenicity of the fungal isolates was tested on dormant canes of the Narince grape cultivars, and the isolates were inoculated using the top inoculation method. Inoculated cuttings were planted in 1-liter pots and grown at 25C and 85 relative humidity for three months. Inhibition distances of 0-13.3 mm between endophytic fungi and Botryosphaeria dothidea, 0-6.3 mm between Cadophora lutea-olivacea, 0-0.7 mm between Cytospora salicacearum, 0-5.0 mm between Diatrype stigma, and 0-8.7 mm between Truncatella angustata occurred. No endophyte inhibited all pathogens to the same extent, with solid effects against some pathogens and weak effects against others. When the inhibition performance of all endophytes was compared with each other, Fusarium avenaceum was the most effective, while Penicillium commune was found to be completely ineffective. Endophytic species did not cause significant necrosis in pathogenicity tests, while B. dothidea, C. lutea-olivacea, C. salicacearum, D. stigma and T. angustata caused necroses of 23.6-65.0 mm. This study indicates that different endophytes in grapevine may be promising for biologically controlling pathogens associated with grapevine trunk diseases.

 Bu çalışma, asma gövde hastalıkları ile ilişkili patojen funguslara karşı endofitik fungusların Tokat koşullarında yetiştirilen asma çeşit ve anaçlarından elde edilen in vitro antagonistik etkilerini belirlemek amacıyla gerçekleştirilmiştir. Antagonistik etkilerin ortaya çıkarılmasında PDA patates dekstroz agar besi yerinde ikili kültür metodu takip edilmiş, izolatlar arasındaki engelleme mesafeleri ölçülmüştür. Fungal izolatların patojenisitesi Narince üzüm çeşidinin dormant kalemleri üzerinde test edilmiş, izolatlar tepeden inokulasyon yöntemine göre uygulanmıştır. İnokule edilmiş kalemler 1 litrelik saksılara dikilmiş, 25C sıcaklık ve 85 nispi nemde 3 ay süreyle büyütülmüştür. Endofitik funguslarla Botryosphaeria dothidea arasında 0-13.3 mm; Cadophora lutea-olivacea arasında 0-6.3 mm; Cytospora salicacearum arasında 0-0.7; Diatrype stigma ile 0-5.0 mm; Truncatella angustata arasında 0-8.7 mmlik engelleme mesafesi meydana gelmiştir. Hiçbir endofit patojenlerin tamamını aynı ölçüde engelleyememiş, bazı patojenlere karşı güçlü bazılarına da zayıf derecede etki ortaya çıkmıştır. Tüm endofitlerin inhibisyon performansı birbiriyle kıyaslandığında Fusarium avenaceumun en etkili, Penicillium commune ise tamamen etkisiz olduğu bulunmuştur. Patojenisite testlerinde endofitik türler kayda değer bir nekroz yaratmazken, B. dothidea, C. lutea-olivacea, C. salicacearum, D. stigma ve T. angustata 23.6-65.0 mmlik nekozlar meydana getirmiştir. Bu çalışma asmadaki farklı endofitlerin, asma gövde hastalıklarıyla ilişkili farklı patojenlerin biyolojik mücadelesi için ümitvar olabileceğine işaret etmektedir.

Article Details

In Vitro Antagonistic Effects of Endophytic Fungi Isolated from Grapevine Rootstocks and Cultivars Against Some of the Fungal Pathogens Associated with Grapevine Trunk Diseases

Authors:

Arife Yağcı | Davut Soner Akgül | Rüstem Cangi

Publisher:

Cukurova University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.451-461

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001337447

pISSN:

2636-7874

biocontrol

endophytic fungi

grapevine trunk diseases

rootstock

vineAsma

anaç

biyokontrol

endofitik funguslar

asma gövde hastalıkları

Issues

Article Details

Authors: Arife Yağcı | Davut Soner Akgül | Rüstem Cangi

Publisher: Cukurova University

Main Topics: Ziraat Mühendisliği (Diğer)

Journal: Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.451-461

Document Type: Article

Publication Date: 12/2024

Document Language: Turkish

Document ID (Org No.): 2001337447

pISSN: 2636-7874

Issues

Authors:

Arife Yağcı | Davut Soner Akgül | Rüstem Cangi

Publisher:

Cukurova University

Main Topics:

Ziraat Mühendisliği (Diğer)

Journal:

Çukurova Tarım ve Gıda Bilimleri Dergisi Vol.39 Issue 2, pp.451-461

Document Type:

Article

Publication Date:

12/2024

Document Language:

Turkish

Document ID (Org No.):

2001337447

pISSN:

2636-7874