Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study

Ahu Pakdemirli; Meral Sarper; Elif Güler Kazancı

Article Details

Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study

Authors:

Ahu Pakdemirli | Meral Sarper | Elif Güler Kazancı

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.507-515

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283442

pISSN:

2564-7288

Keywords:

Abstract:

 Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin, which have the ability to differentiate into other cells in the bone marrow microenvironment under suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health status with personalized homeopathic medicines. At the same time, it is among the certified training programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the literature. As a matter of fact, the material and method parts of these studies are not clear enough. In our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has been presented in detail and it has been aimed to observe their proliferative effects. Material and Methods: Mesenchymal stem cells were exposed to 10, 20, 50 and 100 ratios of homeopathic remedies 30C potency: Aloe, Calendula, Hypericum, Staphysagria for 24 and 48 hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT 3-4,5-dimethyl- thiazolyl-2,5-diphenyltetrazolium bromide test. Results: Aloe vera C 30  Staphysagria C30  Calencula C30  Hypericum C30 remedies were found to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically more significant when maintained in 10 media. Conclusion: It is thought that the methodology used in this study on working methods with homeopathic remedies will contribute to the literature and many scientific studies to be done in the future.

 Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin, which have the ability to differentiate into other cells in the bone marrow microenvironment under suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health status with personalized homeopathic medicines. At the same time, it is among the certified training programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the literature. As a matter of fact, the material and method parts of these studies are not clear enough. In our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has been presented in detail and it has been aimed to observe their proliferative effects. Material and Methods: Mesenchymal stem cells were exposed to 10, 20, 50 and 100 ratios of homeopathic remedies 30C potency: Aloe, Calendula, Hypericum, Staphysagria for 24 and 48 hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT 3-4,5-dimethyl- thiazolyl-2,5-diphenyltetrazolium bromide test. Results: Aloe vera C 30  Staphysagria C30  Calencula C30  Hypericum C30 remedies were found to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically more significant when maintained in 10 media. Conclusion: It is thought that the methodology used in this study on working methods with homeopathic remedies will contribute to the literature and many scientific studies to be done in the future.

 Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin, which have the ability to differentiate into other cells in the bone marrow microenvironment under suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health status with personalized homeopathic medicines. At the same time, it is among the certified training programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the literature. As a matter of fact, the material and method parts of these studies are not clear enough. In our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has been presented in detail and it has been aimed to observe their proliferative effects. Material and Methods: Mesenchymal stem cells were exposed to 10, 20, 50 and 100 ratios of homeopathic remedies 30C potency: Aloe, Calendula, Hypericum, Staphysagria for 24 and 48 hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT 3-4,5-dimethyl- thiazolyl-2,5-diphenyltetrazolium bromide test. Results: Aloe vera C 30  Staphysagria C30  Calencula C30  Hypericum C30 remedies were found to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically more significant when maintained in 10 media. Conclusion: It is thought that the methodology used in this study on working methods with homeopathic remedies will contribute to the literature and many scientific studies to be done in the future.

 Background and purpose: Mesenchymal stem cells are multipotent cells of mesodermal origin, which have the ability to differentiate into other cells in the bone marrow microenvironment under suitable conditions in vitro. Homeopathy is a holistic practice method that aims to improve health status with personalized homeopathic medicines. At the same time, it is among the certified training programs of the Traditional and Complementary Medicine Practices of the Ministry of Health. Studies evaluating the effect of homeopathic remedies in the field of stem cells are rarely encountered in the literature. As a matter of fact, the material and method parts of these studies are not clear enough. In our study, the methodology of working with mesenchymal stem cells of homeopathic remedies has been presented in detail and it has been aimed to observe their proliferative effects. Material and Methods: Mesenchymal stem cells were exposed to 10, 20, 50 and 100 ratios of homeopathic remedies 30C potency: Aloe, Calendula, Hypericum, Staphysagria for 24 and 48 hours. The percent viability of cells at 24 and 48 hours was evaluated with the MTT 3-4,5-dimethyl- thiazolyl-2,5-diphenyltetrazolium bromide test. Results: Aloe vera C 30  Staphysagria C30  Calencula C30  Hypericum C30 remedies were found to have an effect on mesenchymal stem cell proliferation at all doses, and the results were statistically more significant when maintained in 10 media. Conclusion: It is thought that the methodology used in this study on working methods with homeopathic remedies will contribute to the literature and many scientific studies to be done in the future.

Article Details

Effects of Homeopathic Remedies on Mesenchymal Stem Cell Proliferation: A Methodology Study

Authors:

Ahu Pakdemirli | Meral Sarper | Elif Güler Kazancı

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.507-515

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283442

pISSN:

2564-7288

mesenchymal stem cell

homeopathy

remedy

methodology

proliferation

traditional and complementary medicine

mesenchymal stem cell

homeopathy

remedy

methodology

proliferation

traditional and complementary medicine

Issues

Article Details

Authors: Ahu Pakdemirli | Meral Sarper | Elif Güler Kazancı

Publisher: Dokuz Eylul University

Main Topics: Health Care Administration

Journal: Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.507-515

Document Type: Article

Publication Date: 1/2023

Document Language: English

Document ID (Org No.): 2001283442

pISSN: 2564-7288

Issues

Authors:

Ahu Pakdemirli | Meral Sarper | Elif Güler Kazancı

Publisher:

Dokuz Eylul University

Main Topics:

Health Care Administration

Journal:

Journal of Basic and Clinical Health Sciences Vol.7 Issue 1, pp.507-515

Document Type:

Article

Publication Date:

1/2023

Document Language:

English

Document ID (Org No.):

2001283442

pISSN:

2564-7288