Discovery of new herbal anthelmintics from Artemisia Annua L. via in silico molecular docking and in vivo extract application

Dilara Karaman; Ahmet Onur Girişgin; Prof.dr.hulusi Malyer

Article Details

Discovery of new herbal anthelmintics from Artemisia Annua L. via in silico molecular docking and in vivo extract application

Authors:

Dilara Karaman | Ahmet Onur Girişgin | Prof.dr.hulusi Malyer

Publisher:

Yildiz Technical University

Main Topics:

Empirical Software Engineering

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 1, pp.198-210

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312373

pISSN:

1304-7191

Keywords:

Abstract:

 One of the most important factors limiting the growth and development of children is gastro-intestinal helminths. A conscious anthelmintic herbal cure is a rational approach to establish-ing a sustainable public health program in the treatment of oxyurid infections that are mostly seen in children, disrupt development, frequently recur and are asymptomatic. This study aims to investigate the molecular mechanisms of the anthelmintic features of Artemisia annua L. and to compare the antinematodal effect of A. annua L. n-hexane extract with Albendazole ABZ in naturally infected mice. For this purpose, A. annua L. n-hexane extract was extracted and orally administered to Balb-c mice infected with Syphacia obvelata oxyurid species at 300, 600, and 1200 mgkg doses for seven days. Mice were examined for changes in S. obvelata egg numbers on days -7, -1, 1, 3, 5, and 7 by the anal tape method. As a reference drug, ABZ was administered at a dose of 5 mgkg for three days, and as solvent control, corn oil was given in the same way and time as the extract. Some components of A. annua L. were investigated for possible chemical interactions and free energy of binding in Haemonchus contortus β-tubu-lin Hcβ-tubulin protein and rat Carnitine Palmitoyltransferase II RnCPT II enzyme by in silico docking simulations. Stigmasterol and friedelin inhibit the RnCPT II enzyme in silico with 9.43 nM and 13.07 nM Ki values, respectively, while not binding to Hcβ-tubulin. Arte-annuin-B and scopoletin inhibited both RnCPT II and Hcβ-tubulin. A. annua L. n-hexane extract at 1200 mgkg dose reduced oxyurid eggs by 88 on the 7th day. ABZ caused a 65.58 reduction. As the result, arteannuin-B, scopoletin, stigmasterol and friedelin are worthy of isolation and investigation in vitro and in vivo in terms of their anthelmintic effect. They can be evaluated as potential anthelmintic molecules.

 One of the most important factors limiting the growth and development of children is gastro-intestinal helminths. A conscious anthelmintic herbal cure is a rational approach to establish-ing a sustainable public health program in the treatment of oxyurid infections that are mostly seen in children, disrupt development, frequently recur and are asymptomatic. This study aims to investigate the molecular mechanisms of the anthelmintic features of Artemisia annua L. and to compare the antinematodal effect of A. annua L. n-hexane extract with Albendazole ABZ in naturally infected mice. For this purpose, A. annua L. n-hexane extract was extracted and orally administered to Balb-c mice infected with Syphacia obvelata oxyurid species at 300, 600, and 1200 mgkg doses for seven days. Mice were examined for changes in S. obvelata egg numbers on days -7, -1, 1, 3, 5, and 7 by the anal tape method. As a reference drug, ABZ was administered at a dose of 5 mgkg for three days, and as solvent control, corn oil was given in the same way and time as the extract. Some components of A. annua L. were investigated for possible chemical interactions and free energy of binding in Haemonchus contortus β-tubu-lin Hcβ-tubulin protein and rat Carnitine Palmitoyltransferase II RnCPT II enzyme by in silico docking simulations. Stigmasterol and friedelin inhibit the RnCPT II enzyme in silico with 9.43 nM and 13.07 nM Ki values, respectively, while not binding to Hcβ-tubulin. Arte-annuin-B and scopoletin inhibited both RnCPT II and Hcβ-tubulin. A. annua L. n-hexane extract at 1200 mgkg dose reduced oxyurid eggs by 88 on the 7th day. ABZ caused a 65.58 reduction. As the result, arteannuin-B, scopoletin, stigmasterol and friedelin are worthy of isolation and investigation in vitro and in vivo in terms of their anthelmintic effect. They can be evaluated as potential anthelmintic molecules.

 One of the most important factors limiting the growth and development of children is gastro-intestinal helminths. A conscious anthelmintic herbal cure is a rational approach to establish-ing a sustainable public health program in the treatment of oxyurid infections that are mostly seen in children, disrupt development, frequently recur and are asymptomatic. This study aims to investigate the molecular mechanisms of the anthelmintic features of Artemisia annua L. and to compare the antinematodal effect of A. annua L. n-hexane extract with Albendazole ABZ in naturally infected mice. For this purpose, A. annua L. n-hexane extract was extracted and orally administered to Balb-c mice infected with Syphacia obvelata oxyurid species at 300, 600, and 1200 mgkg doses for seven days. Mice were examined for changes in S. obvelata egg numbers on days -7, -1, 1, 3, 5, and 7 by the anal tape method. As a reference drug, ABZ was administered at a dose of 5 mgkg for three days, and as solvent control, corn oil was given in the same way and time as the extract. Some components of A. annua L. were investigated for possible chemical interactions and free energy of binding in Haemonchus contortus β-tubu-lin Hcβ-tubulin protein and rat Carnitine Palmitoyltransferase II RnCPT II enzyme by in silico docking simulations. Stigmasterol and friedelin inhibit the RnCPT II enzyme in silico with 9.43 nM and 13.07 nM Ki values, respectively, while not binding to Hcβ-tubulin. Arte-annuin-B and scopoletin inhibited both RnCPT II and Hcβ-tubulin. A. annua L. n-hexane extract at 1200 mgkg dose reduced oxyurid eggs by 88 on the 7th day. ABZ caused a 65.58 reduction. As the result, arteannuin-B, scopoletin, stigmasterol and friedelin are worthy of isolation and investigation in vitro and in vivo in terms of their anthelmintic effect. They can be evaluated as potential anthelmintic molecules.

 One of the most important factors limiting the growth and development of children is gastro-intestinal helminths. A conscious anthelmintic herbal cure is a rational approach to establish-ing a sustainable public health program in the treatment of oxyurid infections that are mostly seen in children, disrupt development, frequently recur and are asymptomatic. This study aims to investigate the molecular mechanisms of the anthelmintic features of Artemisia annua L. and to compare the antinematodal effect of A. annua L. n-hexane extract with Albendazole ABZ in naturally infected mice. For this purpose, A. annua L. n-hexane extract was extracted and orally administered to Balb-c mice infected with Syphacia obvelata oxyurid species at 300, 600, and 1200 mgkg doses for seven days. Mice were examined for changes in S. obvelata egg numbers on days -7, -1, 1, 3, 5, and 7 by the anal tape method. As a reference drug, ABZ was administered at a dose of 5 mgkg for three days, and as solvent control, corn oil was given in the same way and time as the extract. Some components of A. annua L. were investigated for possible chemical interactions and free energy of binding in Haemonchus contortus β-tubu-lin Hcβ-tubulin protein and rat Carnitine Palmitoyltransferase II RnCPT II enzyme by in silico docking simulations. Stigmasterol and friedelin inhibit the RnCPT II enzyme in silico with 9.43 nM and 13.07 nM Ki values, respectively, while not binding to Hcβ-tubulin. Arte-annuin-B and scopoletin inhibited both RnCPT II and Hcβ-tubulin. A. annua L. n-hexane extract at 1200 mgkg dose reduced oxyurid eggs by 88 on the 7th day. ABZ caused a 65.58 reduction. As the result, arteannuin-B, scopoletin, stigmasterol and friedelin are worthy of isolation and investigation in vitro and in vivo in terms of their anthelmintic effect. They can be evaluated as potential anthelmintic molecules.

Article Details

Discovery of new herbal anthelmintics from Artemisia Annua L. via in silico molecular docking and in vivo extract application

Authors:

Dilara Karaman | Ahmet Onur Girişgin | Prof.dr.hulusi Malyer

Publisher:

Yildiz Technical University

Main Topics:

Empirical Software Engineering

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 1, pp.198-210

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312373

pISSN:

1304-7191

Anthelmintic

Artemisia Annua

Friedelin

In Silico Docking

Stigmasterol

Issues

Article Details

Discovery of new herbal anthelmintics from Artemisia Annua L. via in silico molecular docking and in vivo extract application

Authors: Dilara Karaman | Ahmet Onur Girişgin | Prof.dr.hulusi Malyer

Publisher: Yildiz Technical University

Main Topics: Empirical Software Engineering

Journal: Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 1, pp.198-210

Document Type: Article

Publication Date: 2/2024

Document Language: English

Document ID (Org No.): 2001312373

pISSN: 1304-7191

Anthelmintic

Artemisia Annua

Friedelin

In Silico Docking

Stigmasterol

Issues

Authors:

Dilara Karaman | Ahmet Onur Girişgin | Prof.dr.hulusi Malyer

Publisher:

Yildiz Technical University

Main Topics:

Empirical Software Engineering

Journal:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 1, pp.198-210

Document Type:

Article

Publication Date:

2/2024

Document Language:

English

Document ID (Org No.):

2001312373

pISSN:

1304-7191