The SH-SY5Y Human Cell Line: Hawthorne Berry (Crataegus spp.) Protects against 6-OHDA Induced Neurotoxicity In Vitro Model of Parkinson's Disease

Yeşim Yeni; Ahmet Hacımüftüoğlu

Article Details

The SH-SY5Y Human Cell Line: Hawthorne Berry (Crataegus spp.) Protects against 6-OHDA Induced Neurotoxicity In Vitro Model of Parkinson's Disease

Authors:

Yeşim Yeni | Ahmet Hacımüftüoğlu

Publisher:

İstanbul Gelisim University

Main Topics:

Klinik Tıp Bilimleri (Diğer) | Klinik Tıp Bilimleri

Journal:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 21, pp.881-889

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001308762

pISSN:

2536-4499

Keywords:

Abstract:

 Aim: We purposed to study the neuroprotective effects of Hawthorn berry crataegus spp. extract, which is familiar to have antioxidant and anti-inflammatory features, opposite the neurotoxicity led to by 6-OHDA in SH-SY5Y cells. Method: SH-SY5Y cells were treated with Hawthorn berry 25-50-75 and 100 μgmL for two hours ago 6-OHDA administration. Cells were exposed to 200 µM 6-OHDA for 24 hours to mimic the in vitro Parkinsons disease model. After one day, cell viability was measured by lactate dehydrogenase and 3-4,5 dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide analysis. Oxidative stress was evaluated with tumor necrosis factor-α, interleukin-1β, superoxide dismutase, catalase, glutathione, glutathione peroxidase, myeloperoxidase, and malondialdehyde assays. Results: It was found that the viability rate of Hawthorn berry increased depending on the concentration and the cell viability was 94 at the highest concentration p0.001. Also, 6-OHDA raised lactate dehydrogenase leakage in SH-SY5Y cells p0.001. While 6-OHDA exacerbated oxidative stress by enhancing tumor necrosis factor-α, interleukin-1β, myeloperoxidase, and malondialdehyde p0.001, pretreatment with Hawthorn berry alleviated these toxic effects of 6-OHDA through antioxidant capacity by increasing glutathione peroxidase, superoxide dismutase, catalase and glutathione p0.05, p0.001. In line with all findings, Hawthorn berry attenuated neuronal cell demise in a dose-dependent manner. Conclusion: Considering its neuroprotective role as well as its effects on oxidative stress, Hawthorn berry could be a potential natural bio-medicine to prevent the development of Parkinsons disease.

 Aim: We purposed to study the neuroprotective effects of Hawthorn berry crataegus spp. extract, which is familiar to have antioxidant and anti-inflammatory features, opposite the neurotoxicity led to by 6-OHDA in SH-SY5Y cells. Method: SH-SY5Y cells were treated with Hawthorn berry 25-50-75 and 100 μgmL for two hours ago 6-OHDA administration. Cells were exposed to 200 µM 6-OHDA for 24 hours to mimic the in vitro Parkinsons disease model. After one day, cell viability was measured by lactate dehydrogenase and 3-4,5 dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide analysis. Oxidative stress was evaluated with tumor necrosis factor-α, interleukin-1β, superoxide dismutase, catalase, glutathione, glutathione peroxidase, myeloperoxidase, and malondialdehyde assays. Results: It was found that the viability rate of Hawthorn berry increased depending on the concentration and the cell viability was 94 at the highest concentration p0.001. Also, 6-OHDA raised lactate dehydrogenase leakage in SH-SY5Y cells p0.001. While 6-OHDA exacerbated oxidative stress by enhancing tumor necrosis factor-α, interleukin-1β, myeloperoxidase, and malondialdehyde p0.001, pretreatment with Hawthorn berry alleviated these toxic effects of 6-OHDA through antioxidant capacity by increasing glutathione peroxidase, superoxide dismutase, catalase and glutathione p0.05, p0.001. In line with all findings, Hawthorn berry attenuated neuronal cell demise in a dose-dependent manner. Conclusion: Considering its neuroprotective role as well as its effects on oxidative stress, Hawthorn berry could be a potential natural bio-medicine to prevent the development of Parkinsons disease.

 Aim: We purposed to study the neuroprotective effects of Hawthorn berry crataegus spp. extract, which is familiar to have antioxidant and anti-inflammatory features, opposite the neurotoxicity led to by 6-OHDA in SH-SY5Y cells. Method: SH-SY5Y cells were treated with Hawthorn berry 25-50-75 and 100 μgmL for two hours ago 6-OHDA administration. Cells were exposed to 200 µM 6-OHDA for 24 hours to mimic the in vitro Parkinsons disease model. After one day, cell viability was measured by lactate dehydrogenase and 3-4,5 dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide analysis. Oxidative stress was evaluated with tumor necrosis factor-α, interleukin-1β, superoxide dismutase, catalase, glutathione, glutathione peroxidase, myeloperoxidase, and malondialdehyde assays. Results: It was found that the viability rate of Hawthorn berry increased depending on the concentration and the cell viability was 94 at the highest concentration p0.001. Also, 6-OHDA raised lactate dehydrogenase leakage in SH-SY5Y cells p0.001. While 6-OHDA exacerbated oxidative stress by enhancing tumor necrosis factor-α, interleukin-1β, myeloperoxidase, and malondialdehyde p0.001, pretreatment with Hawthorn berry alleviated these toxic effects of 6-OHDA through antioxidant capacity by increasing glutathione peroxidase, superoxide dismutase, catalase and glutathione p0.05, p0.001. In line with all findings, Hawthorn berry attenuated neuronal cell demise in a dose-dependent manner. Conclusion: Considering its neuroprotective role as well as its effects on oxidative stress, Hawthorn berry could be a potential natural bio-medicine to prevent the development of Parkinsons disease.

 Amaç: Çalışmada antioksidan ve antiinflamatuar özelliklere sahip olduğu bilinen Hawthorn berry crataegus spp. ekstraktın, SH-SY5Y hücrelerinde 6-OHDA ile meydana gelen nörotoksisiteye karşı nöroprotektif etkilerini araştırmayı amaçlanmıştır. Yöntem: SH-SY5Y hücreleri, 6-OHDA uygulamasından önce iki saat boyunca Hawthorn berry 25, 50, 75 ve 100 μgmL ile muamele edildi. Hücreler, in vitro Parkinson hastalığı modelini taklit etmek için 24 saat boyunca 200 uM 6-OHDAya maruz bırakıldı. Bir gün sonra, 3-4,5 Dimetiltiazol-2-il-2,5-difeniltetrazolyum bromür ve laktat dehidrogenaz tahlilleri ile hücre canlılığı belirlendi. Oksidatif stres tümör nekroz faktör-α, interlökin-1β, süperoksit dismutaz, katalaz, glutatyon, glutatyon peroksidaz, miyeloperoksidaz ve malondialdehit analizleri ile değerlendirildi. Bulgular: Canlılık oranında Hawthorn berrynin konsantrasyona bağlı olarak bir artış gösterdiği ve en yüksek konsantrasyonda hücre canlılığı  94 oranında bulundu p0,001. Ayrıca, 6-OHDA SH-SY5Y hücrelerinde laktat dehidrogenaz sızıntısını arttırdı p0,001. 6-OHDA tümör nekroz faktör-α, interlökin-1β, miyeloperoksidaz ve malondialdehit artırarak oksidatif stresi şiddetlendirirken p0,001, Hawthorn berry ile ön tedavi süperoksit dismutaz, katalaz, glutatyon ve glutatyon peroksidazı artırarak antioksidan kapasite yoluyla 6-OHDAnın bu toksik etkilerini hafifletti p0,05, p0,001. Tüm bulgular doğrultusunda Hawthorn berry nöronal hücre ölümünü hafifleterek doza bağlı bir şekilde önledi. Sonuç: Oksidatif stres üzerindeki etkilerinin yanı sıra nöroprotektif rolü göz önüne alındığında Hawthorn berry, Parkinson hastalığının gelişimini önlemek için potansiyel doğal biyo-ilaç olabilir.

Article Details

The SH-SY5Y Human Cell Line: Hawthorne Berry (Crataegus spp.) Protects against 6-OHDA Induced Neurotoxicity In Vitro Model of Parkinson's Disease

Authors:

Yeşim Yeni | Ahmet Hacımüftüoğlu

Publisher:

İstanbul Gelisim University

Main Topics:

Klinik Tıp Bilimleri (Diğer) | Klinik Tıp Bilimleri

Journal:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 21, pp.881-889

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001308762

pISSN:

2536-4499

Antioxidant

Hawthorn berry

6-OHDA

Parkinson’s disease

SH-SY5Y cell lineAntioksidan

Hawthorn berry

6-OHDA

Parkinson hastalığı

SH-SY5Y hücre hattı

Issues

Article Details

Authors: Yeşim Yeni | Ahmet Hacımüftüoğlu

Publisher: İstanbul Gelisim University

Main Topics: Klinik Tıp Bilimleri (Diğer) | Klinik Tıp Bilimleri

Journal: İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 21, pp.881-889

Document Type: Article

Publication Date: 5/2024

Document Language: English

Document ID (Org No.): 2001308762

pISSN: 2536-4499

Issues

Authors:

Yeşim Yeni | Ahmet Hacımüftüoğlu

Publisher:

İstanbul Gelisim University

Main Topics:

Klinik Tıp Bilimleri (Diğer) | Klinik Tıp Bilimleri

Journal:

İstanbul Gelişim Üniversitesi Sağlık Bilimleri Dergisi Issue 21, pp.881-889

Document Type:

Article

Publication Date:

5/2024

Document Language:

English

Document ID (Org No.):

2001308762

pISSN:

2536-4499