Association between Matrix Metalloproteinas-2 Gene Variants and Pathogenesis of Breast Cancer in Sera of Iraqi Women

Yammamah Jawad Abbas; Fadhil Jawad Al-Tu'ma; Alaa Fraq Al-Hemerri

doi:10.22317/jcms.v6i6.891

Article Details

Association between Matrix Metalloproteinas-2 Gene Variants and Pathogenesis of Breast Cancer in Sera of Iraqi Women

Authors:

Yammamah Jawad Abbas | Fadhil Jawad Al-Tu'ma | Alaa Fraq Al-Hemerri

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.6 Issue 6, pp.285-290

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001379384

pISSN:

2413-0516

DOI:

10.22317/jcms.v6i6.891

Keywords:

Abstract:

 Objective: The current study aims to investigate the role of MMP-2 in breast cancer pathogenesis in Iraqi women.
Methods: A forty one women with breast cancer and 45 control women were included in this case-control study. Body mass index, age, smoking; married status, tumor size, degree, subtype, lymph node status, pre and postmenopesua included the phenotypic results. The polymerase chain reaction-PCR-allele specific restriction was used to observe the rs243865 polymorphism. Genomic DNA was extracted from whole blood and genotyping with specific prefixes for amplification of the MMP-2 gene was accomplished as enzyme-restricted PCR products were digested, followed by electrophoresis on 1.5% agarose gel. In order to interpret the researchers' results, numerous statistical analyses were applied.
Results: The amplicon size of MMP-2 gene was 304 bp, and following its amplification reactions by allelic specific PCR. The amplification product for MMP-2 gene amplification SNP rs243865 gene polymorphism results exhibited one band of 304 bp, two bands of 304 bp and one band 304 bp for individuals have genotype as wild type (CC), homozygous (TT) and heterozygous (CT), respectively. Genotype frequencies of rs243865 polymorphism were found to be consistent with Hardyâ€“Weinberg equilibrium. Allele frequencies of C allele was 0.57, and the T allele was only 0.43 in cases of breast cancer women patient, while the frequencies of CC, CT, and TT genotypes of the rs243865 SNP were statistically significant as 31.7%, 51.2%, 17.1% respectively. Allele frequencies of C and T were 0.78 and 0.22 for the control group, respectively, the heterozygous genotype (CT) was significantly increased the risk of breast cancer women (OR=0.3, 95% CI;0.12 â€“ 0.8 , Pâ‰¤ 0.05) with respect to those of the CC wild type.  The TT genotype significantly raised the risk of breast cancer women by (OR = 0.2, 95% CI; 0.04 â€“ 0.9, Pâ‰¤ 0.05).
Conclusion: In women with breast cancer, MMP-2 expression is highly association were observed with positive lymph node, histological classification of breast cancer (ll) higher than other classes, and advanced clinical process (ll).

Article Details

Association between Matrix Metalloproteinas-2 Gene Variants and Pathogenesis of Breast Cancer in Sera of Iraqi Women

Authors:

Yammamah Jawad Abbas | Fadhil Jawad Al-Tu'ma | Alaa Fraq Al-Hemerri

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.6 Issue 6, pp.285-290

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001379384

pISSN:

2413-0516

DOI:

10.22317/jcms.v6i6.891

MMP-2

Breast cancer

Allele

Genotype

Polymerase Chain Reaction

SNP

Issues

Article Details

Association between Matrix Metalloproteinas-2 Gene Variants and Pathogenesis of Breast Cancer in Sera of Iraqi Women

Authors: Yammamah Jawad Abbas | Fadhil Jawad Al-Tu'ma | Alaa Fraq Al-Hemerri

Publisher: Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal: Journal of Contemporary Medical Sciences Vol.6 Issue 6, pp.285-290

Document Type: Article

Publication Date: 12/2020

Document Language: English

Document ID (Org No.): 2001379384

pISSN: 2413-0516

DOI: 10.22317/jcms.v6i6.891

MMP-2

Breast cancer

Allele

Genotype

Polymerase Chain Reaction

SNP

Issues

Authors:

Yammamah Jawad Abbas | Fadhil Jawad Al-Tu'ma | Alaa Fraq Al-Hemerri

Publisher:

Nab'a Al-Hayat Foundation for Medical Sciences and Health Care, Iraq

Journal:

Journal of Contemporary Medical Sciences Vol.6 Issue 6, pp.285-290

Document Type:

Article

Publication Date:

12/2020

Document Language:

English

Document ID (Org No.):

2001379384

pISSN:

2413-0516

DOI:

10.22317/jcms.v6i6.891